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Sc 29455

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

Sc-29455 is a laboratory equipment product offered by Santa Cruz Biotechnology. It serves as a core function for specific applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Amaxa human keratinocyte nucleofector kit, by Lonza (2 mentions) Sc 44234, by Santa Cruz Biotechnology (2 mentions) Allstars negative sirna control, by Qiagen (1 mentions) Lipofactamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions) Amaxa human fibroblasts nucleofector kit, by Lonza (1 mentions) Sc 37007, by Santa Cruz Biotechnology (1 mentions)

4 protocols using sc 29455

1

Knockdown of PPARγ in Caco-2 Cells

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A 50 nM of small interfering RNA (siRNA) oligonucleotides against PPARγ (sc-29455, Santa Cruz Biotechnology) were transfected into Caco-2 cells in a 12 well plate (1.5 X 105 cells/well) by using Lipofactamine RNAimax transfection reagent (Invitrogen). Control cells were transfected with Allstars negative siRNA control (Qiagen). The knockdown efficiency of PPARγ siRNA was confirmed by western blotting.
Hyun J., Romero L., Riveron R., Flores C., Kanagavelu S., Chung K.D., Alonso A., Sotolongo J., Ruiz J., Manukyan A., Chun S., Singh G., Salas P., Targan S.R, & Fukata M. (2014). Human Intestinal Epithelial Cells Express Interleukin-10 through Toll-Like Receptor 4-Mediated Epithelial-Macrophage Crosstalk. Journal of Innate Immunity, 7(1), 87-101.
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2

RNA Interference Experiments in NHKs

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For the RNA interference experiments, NHKs were transfected with 100 pmols (h) siRNA specific for PPARγ (sc-29455; Santa Cruz Biotechnology, Santa Cruz, CA, USA). An equivalent amount of non-specific siRNA (sc-44234; Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used as a negative control. Cells were transfected using Amaxa® human keratinocyte Nucleofector kit (Lonza, Basel, Switzerland), according to manufacturer’s instructions. To ensure identical siRNA efficiency among the plates, cells were transfected together in a single cuvette and plated immediately after nucleofection. 24 h following transfection, treatments were added to some samples in agreement with the experimental design.
Flori E., Mastrofrancesco A., Kovacs D., Bellei B., Briganti S., Maresca V., Cardinali G, & Picardo M. (2017). The activation of PPARγ by 2,4,6-Octatrienoic acid protects human keratinocytes from UVR-induced damages. Scientific Reports, 7, 9241.
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3

Efficient Knockdown of PPARγ in Fibroblasts

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For the RNA interference experiments, HDFs were transfected with 100 pmol (h) siRNA specific for PPARγ (sc-29455; Santa Cruz Biotechnology). An equivalent amount of non-specific siRNA (sc-44234; Santa Cruz Biotechnology) was used as a negative control. Cells were transfected using the Amaxa human fibroblasts Nucleofector kit (Lonza) according to manufacturer's instructions. To ensure identical siRNA efficiency among the plates, cells were transfected together in a single cuvette and plated immediately after nucleofection. Twenty-four hours following transfection, HDFs were treated with PUVA and post-incubated with 2µM Octa in agreement with the experimental design.
Briganti S., Flori E., Bellei B, & Picardo M. (2014). Modulation of PPARγ Provides New Insights in a Stress Induced Premature Senescence Model. PLoS ONE, 9(8), e104045.
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4

RNA Interference Silencing of PPARγ in A431 Cells

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For the RNA interference experiments, A431 cells were transfected with 100 pmol siRNA specific for PPARγ (sc-29455; Santa Cruz Biotechnology, Santa Cruz, CA, USA). An equivalent amount of non-specific siRNA (sc-37007; Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used as a negative control. Cells were transfected using Amaxa human keratinocyte Nucleofector kit (Lonza, Basel, Switzerland), according to the manufacturer’s instructions. To ensure identical siRNA efficiency among the plates, cells were transfected together in a single cuvette and plated immediately after nucleofection. Twenty-four hours after transfection, treatments were added to some samples in agreement with the experimental design.
Flori E., Mosca S., Cardinali G., Briganti S., Ottaviani M., Kovacs D., Manni I., Truglio M., Mastrofrancesco A., Zaccarini M., Cota C., Piaggio G, & Picardo M. (2023). The Activation of PPARγ by (2Z,4E,6E)-2-methoxyocta-2,4,6-trienoic Acid Counteracts the Epithelial–Mesenchymal Transition Process in Skin Carcinogenesis. Cells, 12(7), 1007.
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