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2 protocols using rabbit anti mouse hrp antibody

1

Protein-Anhydride Interactions for Biomedical Applications

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3-hydroxyphthalic anhydride (HP), maleic anhydride (ML), succinic anhydride (SU), human serum albumin (HSA), chicken ovalbumin (OVA), bovine serum albumin (BSA), and β-lactoglobulin (β-LG) were purchased from Sigma (St. Louis, MO, USA). Cell Counting Kit-8 (CCK-8) was bought from Dojindo Molecular Technologies, Inc. (Dojindo, Japan). 2,4,6-trinitrobenzenesulfonic acid (TNBS) was purchased from Sigma. ρ-hydroxyphenylglyoxal (ρ-HPG) was purchased from Fisher Scientific Co. (Valley Park, VA, USA). BMS433771 and TMC353121 were purchased from Sigma. Anti-RSV antibody (NCL-RSV3) was from Leica Biosystems (Leica Biosystems Newcastle Ltd., Newcastle Upon Tyne, UK). Rabbit anti-mouse HRP antibody was purchased from Dako (Dako, Glostrup, Denmark). Low melting point agarose was bought from Invitrogen (Invitrogen, Carlsbad, CA, USA).
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2

Inhibition of Lysosomal Protease Activity

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(Z‐Leu‐Leu‐NHCH2)2CO, 1,3‐di‐(N‐carboxybenzoyl‐ l‐leucyl‐ l‐leucyl)amino acetone, and 2,2′‐(2‐oxo‐1,3‐propanediyl)bis[N‐[(phenylmethoxy)carbonyl]‐ l‐leucyl‐ l‐leucinamide) ((Z‐LL2)‐ketone) were purchased from Santa Cruz (sc‐311559); N‐morpholinurea–leucine–homophenylalanine–vinylsulfone‐phenol (LHVS) was synthesized in the laboratory of Dr. C Driessen, Department of Oncology and Hematology, Cantonal Hospital St Gallen, St Gallen, Switzerland, following protocols as published in [27]. Recombinant IFN‐γ was purchased from eBioscience (34‐8319‐82). The following antibodies were used: mouse anti‐human LAMP‐1 PE (BD; clone H4A3; 555801), mouse anti‐human CLIP FITC (BD; clone CerCLIP; 555981) mouse anti‐human CD74 PE (Biolegend; clone LN2; 326807), mouse anti‐human CD14 Pacific blue (Biolegend; clone M5E2; 301815), mouse anti‐human HLA ABC PE‐Cy7 (BD; clone G46‐2.6; 561349), mouse anti‐human HLA DR PerCP (Biolegend; clone L243; 560896), mouse anti‐human CD74 N‐terminal (Abcam; clone 2D1B3; ab181465), goat anti‐human β actin (Santa Cruz; clone I‐19; SC1616), goat anti‐mouse AF647 (Thermofisher Scientific; A28181), rabbit anti‐human Na‐K ATPase (Cell Signalling; 3010s), mouse anti‐human LAMP1 (BD, clone H4A3; 555798), Rabbit Anti‐mouse‐HRP antibody, (Dako; P0161), and Rabbit anti‐goat HRP antibody (Dako; P0160).
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