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Anti gabarap

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Anti-GABARAP is an antibody that recognizes the GABA receptor-associated protein (GABARAP), a small protein involved in the trafficking and anchoring of GABA receptors. It is commonly used in research to study the localization and interactions of GABARAP in cells and tissues.

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Lab products found in correlation

Anti ha, by Merck Group (2 mentions) Anti flag m2, by Merck Group (2 mentions) Anti sting, by Cell Signaling Technology (1 mentions) Anti vamp8, by Abcam (1 mentions) Anti acc, by Cell Signaling Technology (1 mentions) Anti p raptor ser792, by Cell Signaling Technology (1 mentions) Anti lamp1, by Merck Group (1 mentions) Anti lamp2, by Santa Cruz Biotechnology (1 mentions) Anti laminin 2, by Merck Group (1 mentions) Anti tsc2, by Cell Signaling Technology (1 mentions) Anti tbc1d1, by Cell Signaling Technology (1 mentions) Anti fip200, by Proteintech (1 mentions) Anti egfp gl 8, by Takara Bio (1 mentions) Anti stx17 hpa001204, by Merck Group (1 mentions) Anti wipi2, by Abcam (1 mentions) Anti pacc ser79, by Cell Signaling Technology (1 mentions) Lysotracker, by Enzo Life Sciences (1 mentions) Anti dystrophin, by Abcam (1 mentions) Anti p ampk t172, by Cell Signaling Technology (1 mentions) Anti cd36, by Abcam (1 mentions)

4 protocols using anti gabarap

1

Comprehensive Antibody Collection for Cell Signaling

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Antibodies used in this study included anti-STING (13647; Cell Signaling Technology), anti-Flag M2 (F3165; Sigma-Aldrich), anti-HA (H3663; Sigma-Aldrich), anti-EGFP (GL-8; Clontech), anti-LC3 (7543; Sigma-Aldrich), anti-p62 (PM045; MBL), anti-STX17 (HPA001204; Sigma-Aldrich), anti-SNAP29 antibody (111303, SYSY, or sc-135564; Santa Cruz Biotechnology), anti-LAMP1(L1418; Sigma-Aldrich), anti-LAMP2 (sc-18822; Santa Cruz or L0668; Sigma-Aldrich), anti-Myc (9E10, DSHB), anti-WIPI2 (ab105459; Abcam), anti-FIP200 (17250; ProteinTech), anti-ATG16L (PM040; MBL), anti-α-Tubulin (E7; DSHB), anti-VAMP8 (ab76021; Abcam), anti-GABARAP (ab109364; Abcam), anti-AMPK (2532; Cell Signaling Technology), anti-p-AMPK T172 (2535; Cell Signaling Technology), anti-TBC1D1(66433; Cell Signaling Technology), anti-p-TBC1D1 Ser237(07-2268; Sigma-Aldrich), anti-Raptor (2280; Cell Signaling Technology), anti-p-Raptor Ser792 (2083; Cell Signaling Technology), anti-TSC2 (4308; Cell Signaling Technology), anti-p-TSC2 Ser1387 (5584; Cell Signaling Technology), anti-ACC (3662; Cell Signaling Technology), anti-p-ACC Ser79 (3661; Cell Signaling Technology), anti-Glut4 (GT-41-A; Alpha diagnostic international), anti-Laminin2 (L0663; Sigma-Aldrich), anti-CD36 (80080; Abcam), anti-Dystrophin (15277; Abcam), and LysoTracker (ENZ-51005; Enzo).
Rong Y., Zhang S., Nandi N., Wu Z., Li L., Liu Y., Wei Y., Zhao Y., Yuan W., Zhou C., Xiao G., Levine B., Yan N., Mou S., Deng L., Tang Z., Liu X., Kramer H, & Zhong Q. (2022). STING controls energy stress-induced autophagy and energy metabolism via STX17. The Journal of Cell Biology, 221(7), e202202060.
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2

Autophagy Pathway Protein Detection

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Anti-LC3B (#ab48394, Abcam), anti-β-actin (#ab6276, Abcam), anti-ATG4B (#A2981, Sigma), anti-p62 (#P0067, Sigma), anti-GABARAP (#ab109364, Abcam), anti-vinculin (#ab129002, Abcam), goat anti-mouse IgG–horseradish peroxidase (HRP), and goat anti-rabbit IgG–HRP (Santa Cruz Biotechnology) antibodies were used in immunoblotting. Wortmannin (Sigma-Aldrich) was used to block autophagosome formation and bafilomycin A1 (Sigma-Aldrich) was used for autophagy flux assays.
Bosc D., Vezenkov L., Bortnik S., An J., Xu J., Choutka C., Hannigan A.M., Kovacic S., Loo S., Clark P.G., Chen G., Guay-Ross R.N., Yang K., Dragowska W.H., Zhang F., Go N.E., Leung A., Honson N.S., Pfeifer T.A., Gleave M., Bally M., Jones S.J., Gorski S.M, & Young R.N. (2018). A new quinoline-based chemical probe inhibits the autophagy-related cysteine protease ATG4B. Scientific Reports, 8, 11653.
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3

Antibodies for Autophagy Protein Analysis

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Antibodies used for this study were anti-LC3 (Cell Signaling, 4108), anti-LC3 (Novus, NB100-2220), anti-Ref(2)P (Abcam, ab178440), anti-GABARAP (Abcam, ab109364), anti-pULK1-Ser555 (Cell signaling, 5869), anti-ULK1 (Cell signaling, 8054), anti-USP5 (Proteintech, 10473-1-AP), anti-p62 (MBL, PM045), anti-Flag M2 (Sigma, F1804), anti-MYC (Santa Cruz, SC-40), anti-HA (Sigma, H9658), anti-ACTB/β-actin (Novus, NB600-501), anti-tubulin (Sigma, T0198), anti-GFP (Abcam, ab290), anti-ubiquitin (FK2; Sigma, ST1200), and anti-ubiquitin (P4D1; Cell signaling, 3936). To generate antiserum against Drosophila Atg1, the C-terminal segment of Atg1 (amino acid residues 562-855) was cloned to pET32a vector to generate 6xHis-tagged fusion protein. The fusion protein was purified using a Ni Sephsrose 6 FF column (GE Healthcare). The purified protein was used to immunize rabbits by LTK BioLaboratories and the resulting antiserum was purified by NAb Protein A Plus Spin Kit (Thermo).
Pai Y.L., Lin Y.J., Peng W.H., Huang L.T., Chou H.Y., Wang C.H., Chien C.T, & Chen G.C. (2023). The deubiquitinase Leon/USP5 interacts with Atg1/ULK1 and antagonizes autophagy. Cell Death & Disease, 14(8), 540.
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4

Antibody immunoblotting protocol

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Primary antibodies were obtained from the following sources: anti-ATXN2 (mouse monoclonal, 1:500; BD biosciences, Franklin Lakes, NJ) anti-ATXN3 (mouse monoclonal 1H9, MAB5360, 1:500–1000; Millipore, Burlington, MA), anti-dSesn (rabbit polyclonal, 1:500, Lee et al., 2010 (link)), anti-GABARAP (rabbit polyclonal, 1:1000, Abcam, Cambridge, United Kingdom). Peroxidase-conjugated secondary antibodies (goat anti-mouse, goat anti-rabbit, 1:5000; Jackson Immunoresearch, West Grove, PA).
Sujkowski A., Richardson K., Prifti M.V., Wessells R.J, & Todi S.V. (2022). Endurance exercise ameliorates phenotypes in Drosophila models of spinocerebellar ataxias. eLife, 11, e75389.
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