Spectrum one ftir spectrometer

All films were used for FTIR analysis after being dried out for two weeks in a desiccator. A Perkin Elmer FTIR Spectrometer Spectrum One (Perkin Elmer, Waltham, MA, USA) operated at a resolution of 4 cm⁻¹ was used to analyze the FTIR spectra of the chitosan blended films. The FTIR spectra were recorded in the range of 4000–650 cm⁻¹ wavenumbers with 64 scans.

FTIR spectra of human hair, nail, bovine hoof and KF-110 were measured using a FTIR Spectrometer Spectrum One (Perkin Elmer, Waltham, MA, USA). The spectra were registered from 400 to 4000 cm⁻¹ by 16 scans. The samples were mixed with KBr (Spectrum, Stamford, CT, USA) at a 1:100 (w/w) sample to KBr ratio and ground vigorously. The pellets were formed using a press (Equilab, Madrid, Spain) under 5000 kg_f for 2 min with the help of a 13 mm pellet die (Carver, Wabash, IN, USA).

¹H-NMR spectra were recorded at room temperature at 500 MHz (128 scans per analysis) on an Agilent VNMRS 500 spectrometer in CDCl₃, using tetramethyl silane as an internal standard. ²⁹Si-NMR spectra were recorded at room temperature using glass tubes at 500 MHz (256 scans; relax time of 30 s) on an Agilent VNMRS 500 spectrometer in CDCl₃, without tetramethyl silane as an internal standard. Gel permeation chromatography (GPC) measurements were performed on a TOSOH EcoSEC GPC system equipped with an auto sampler system, a temperature-controlled pump, a column oven, a refractive index (RI) detector, a purge and degasser unit, and a TSKgel superhZ2000, 4.6 mm ID × 15 cm × 2 cm column. Tetrahydrofuran was used as an eluent at a flow rate of 1.0 mL.min⁻¹ at 40 °C. The refractive index detector was calibrated with polystyrene standards, having narrow molecular weight distributions. The data were analyzed using Eco-SEC analysis software. The Fourier transform infrared (FTIR) spectroscopy measurements were recorded on a PerkinElmer FTIR Spectrum One spectrometer. Viscosimetry experiments were conducted at room temperature via Ubbelohde Apparatus SI Analytics Typ 501 01/0a Appr. Nr. 1068985. 5.

The phytochemical compositions of the plant extracts were separated using classical column chromatographic techniques. Then, the isolated compounds were elucidated via ¹H and ¹³C Nuclear Magnetic Resonance spectrometry (1D and 2D NMR) monitoring with a Fourier Transform NMR Spectrometer (¹H-NMR 500 MHz and ¹³C-NMR 125 MHz), model UNITY INNOVA, Varian (Scientific Equipment Center, Prince of Songkla University). The UV spectra were obtained using a Spectronic Genesys 6 UV-Visible Spectrometer, Thermo Scientific, Thermo Electron Corporation, and IR (KBr disc) spectra were obtained using a Perkin Elmer FT-IR Spectrum One Spectrometer (Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University).

IR spectra of isolated compounds were measured by Perkin Elmer FT-IR Spectrum One Spectrometer. NMR spectra were obtained by Fourier Transform NMR Spectrometer (¹H-NMR 500 MHz and ¹³C-NMR 125 MHz), model UNITY INNOVA, Varian. Electron spray ionization mode (ESI) of MS was observed on Alliance-micromass Waters 2690-LCT

All ¹H NMR spectra were recorded on an Agilent NMR System VNMRS 500 spectrometer at room temperature in CDCl₃ or DMSO-d₆ with Si(CH₃)₄ as an internal standard. FTIR spectra were recorded on a Perkin-Elmer FTIR Spectrum One spectrometer. Differential Scanning Calorimetry (DSC) was performed on Perkin-Elmer Diamond DSC from 30 °C to 320 °C with a heating rate of 10 °C min⁻¹. under nitrogen flow. A typical DSC sample was 2–5 mg in a 30 μL aluminum pan. Thermal gravimetric analysis (TGA) was performed on Perkin-Elmer Diamond TA/TGA with a heating rate of 10 °C min under nitrogen flow. Uniaxial elongation measurements were performed on polymeric film samples (approx. 10 mm length and 2.5 mm² cross-section area). Measurements were carried out using a Perkin Elmer Pyris Diamond DMA (SII Nanotechnology Inc.) at 25 °C under 50 mN/min. load speed. The tensile strength and percentage elongation at break were recorded.

¹H NMR spectra were recorded using an Agilent VNMRS 500 MHz (Santa Clara, CA, USA), and chemical shifts were recorded in ppm using tetramethylsilane as an internal standard. FTIR spectra were recorded on a PerkinElmer FTIR Spectrum One spectrometer (MA, USA). Differential scanning calorimetry (DSC) was performed on PerkinElmer Diamond DSC (MA, USA) from 20 to 320 °C with a heating rate of 10 °C min⁻¹ under nitrogen flow. Thermal gravimetric analysis (TGA) was performed on PerkinElmer Diamond TA/TGA with a heating rate of 10 °C min⁻¹ under nitrogen flow. Molecular weights were determined by gel permeation chromatography (GPC). The measurements were performed on a TOSOH EcoSEC GPC system (MA, USA) equipped with an autosampler system, a temperature-controlled pump, a column oven, a refractive index (RI) detector, a purge and degasser unit, and a TSKgel SuperHZ2000, 4.6 mm i.d. × 15 cm × 2 cm column. Tetrahydrofuran was used as an eluent at a flow rate of 1.0 mL min⁻¹ at 40 °C. The refractive index detector was calibrated with polystyrene standards which had narrow molecular weight distributions. Data were analyzed using EcoSEC analysis software.