Seven- to eight-week-old female C57BL/6 mice (BioLASCO Taiwan Co., Ltd) or A/J mice (Japan SLC, Inc.) were used to produce the murine intranasal infection model. C. neoformans or C. deuterogattii strains were cultured in YPD broth overnight at 30 °C with shaking at 200 rpm, and washed twice with PBS. Cells were counted with hemocytometer, resuspended in PBS, and diluted to 106 cells (for C57BL/6 mice) or 107 cells (for A/J mice) per ml. Appropriate dilutions of the cells were plated onto YPD agar plate and incubated at 30 °C for 48 h in order to confirm CFU numbers and viability. Groups of 10 (C57BL/6) or 7 (A/J) mice were anesthetised with isoflurane (Panion & BF Biotech Inc.) for C57BL/6 mice or 2% tribromoethanol (T48402, Sigma-Aldrich) for A/J mice, and inoculated with C. neoformans or C. deuterogattii strains via intranasal instillation of inocula of 5 × 104 cells (for C57BL/6 mice) or 5 × 105 cells (for A/J mice) in 50 µl PBS. The survival of mice was monitored twice daily for 49 days, and moribund mice (mice that were unable to eat or drink, their body weight was reduced by 15%, or were hunched) were euthanised with CO2. The survival curve was created with Prism 8. The significance of differences was determined using Log-rank (Mantel-Cox) test.
C57bl 6 mice
Manufactured by Merck Group
Sourced in United States, China
The C57BL/6 mouse is a widely used inbred strain that serves as a standard laboratory mouse model. It is a common control strain in many studies due to its well-characterized genetic background. These mice can be used for a variety of research applications, including but not limited to immunology, neuroscience, and metabolic studies.
Automatically generated - may contain errors
Lab products found in correlation
Streptozotocin (stz), by Merck Group (7 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (6 mentions)
Cuprizone, by Merck Group (4 mentions)
Scopolamine hydrobromide, by Merck Group (2 mentions)
Etomoxir, by Merck Group (2 mentions)
E coli 0111 b4, by Merck Group (2 mentions)
Cocaine hcl, by Merck Group (2 mentions)
Gsk j4, by Bio-Techne (1 mentions)
Elisa kits for tnf α, by R&D Systems (1 mentions)
Mcp 1 ccl2, by R&D Systems (1 mentions)
Secondary antibodies conjugated to horseradish peroxidase, by Bio-Rad (1 mentions)
Rotamex rotarod, by Columbus Instruments (1 mentions)
Il 1β, by R&D Systems (1 mentions)
Bacterial lps, by Merck Group (1 mentions)
μct100, by Scanco (1 mentions)
Ss jrhsdmcwicrl, by Charles River Laboratories (1 mentions)
Wild type c57bl 6 mice, by Charles River Laboratories (1 mentions)
Ang 2, by Merck Group (1 mentions)
Alginic acid, by Merck Group (1 mentions)
C57bl 6, by Charles River Laboratories (1 mentions)
38 protocols using c57bl 6 mice
1
Murine Intranasal Infection Model for Cryptococcosis
2
C57BL/6 and IL33-/- Mice Protocol
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C57BL/6 mice were purchased from the Jackson Laboratory (Bar Harbor, ME). IL33-/- mice on C57BL/6 background were kindly provided by Dr. Rene de Waal Malefyt (Merck, Palo Alto, CA), and bred with WT C57BL/6 mice. WT and IL33-/- littermates were cohoused and used as indicated in the text. All mice were housed under specific pathogen-free conditions at the animal resource center of University of Texas Medical Branch at Galveston. All experiments were reviewed and approved by the Institutional Animal Care and Use Committee of University of Texas Medical Branch at Galveston.
3
Inducing Diabetes in C57BL/6 Mice and Evaluating GSK-J4 Treatment
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Diabetes mellitus was induced in 12-week-old male C57BL/6 mice (BioLasco Biotechnology Co., Taiwan) through an intraperitoneal injection of STZ (200 mg/kg body weight) according to the standard method [42 (link)]. Before injection, the STZ (Merck, St. Louis, MO, USA) was dissolved in a sodium citrate buffer (pH 4.5) to a final concentration of 20 mg/mL. After the intraperitoneal injection of STZ into the C57BL/6 mice, normal chow and water of 10% sucrose were also provided. On day 3, the 10% sucrose water was replaced with regular water. On day 10, the mice fasted for 6 h, then, tail vein blood samples were collected to measure the blood glucose. Mice were analyzed for three consecutive analog fasting glucose concentrations at 200–300 mg/dL, confirming that the mice were diabetic, as previously reported [15 (link)].
This experiment was divided into three experimental groups, with six mice in each group; Group 1: normal mice; Group 2: STZ-induced diabetic mice; and Group 3: STZ-induced diabetic mice that were treated with GSK-J4. GSK-J4 (0.4 mg/kg, 4594; Tocris Bioscience, Bristol, UK) was administered subcutaneously daily for one week. In the fifth week after the experimental treatment, the kidney tissue of each mouse was collected for subsequent analysis.
This experiment was divided into three experimental groups, with six mice in each group; Group 1: normal mice; Group 2: STZ-induced diabetic mice; and Group 3: STZ-induced diabetic mice that were treated with GSK-J4. GSK-J4 (0.4 mg/kg, 4594; Tocris Bioscience, Bristol, UK) was administered subcutaneously daily for one week. In the fifth week after the experimental treatment, the kidney tissue of each mouse was collected for subsequent analysis.
4
Cuprizone-Induced Demyelination Protocol
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Male C57BL/6 mice (8 weeks old) were purchased from Spelford (Beijing) Biotechnology Company. Mice were housed in an air-conditioned room at 22 ± 1°C with a 12-h light-dark cycle (lights on from 7:00 a.m. to 7:00 p.m.) (Zhao et al., 2021 (link)). Food and tap water were freely available. All procedures for the following experiments were approved by the Animal Care and Use Committee, performed by the NIH Guide for the Care and Use of Laboratory Animals.
Twenty male C57BL/6 mice (8 weeks old) were randomly divided into the control group (n = 10) and the cuprizone (CPZ) group (n = 10), and mice in the CPZ group were fed by a diet containing 0.2% cuprizone (reagent purchased from Sigma, custom-made feed from Jiangsu Hershey Feeds). By referring to the literature (Wellman et al., 2020 (link)), C57BL/6 male mice were selected to be fed 0.2% cuprizone chow to construct the demyelination model, and we chose continuous feeding (11 weeks) for the CPZ group (Zhao et al., 2021 (link)). Then, mice were executed, and the corpus callosum was immediately stored at −80°C for subsequent detection of the targeted lipidomics and transcriptomics.
Twenty male C57BL/6 mice (8 weeks old) were randomly divided into the control group (n = 10) and the cuprizone (CPZ) group (n = 10), and mice in the CPZ group were fed by a diet containing 0.2% cuprizone (reagent purchased from Sigma, custom-made feed from Jiangsu Hershey Feeds). By referring to the literature (Wellman et al., 2020 (link)), C57BL/6 male mice were selected to be fed 0.2% cuprizone chow to construct the demyelination model, and we chose continuous feeding (11 weeks) for the CPZ group (Zhao et al., 2021 (link)). Then, mice were executed, and the corpus callosum was immediately stored at −80°C for subsequent detection of the targeted lipidomics and transcriptomics.
5
Desiccating Stress Dry Eye Model in Mice
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All studies performed were approved by The Institutional Animal Care and Use Committees at Baylor College of Medicine and comply with the ARVO Statement for the Use of Animals in Vision Research. Female C57BL/6 mice aged 6–8 weeks old were purchased from Jackson Laboratories (Bar Harbor, ME). DS was induced by subcutaneous injection of scopolamine hydrobromide (0.5 mg/0.2 ml; Sigma-Aldrich, St. Louis), QID (08:00, 12:00, 14:00, and 17:00 h), for 3, 5 or 10 consecutive days in 6–8 week old female C57BL/6 mice and euthanized at the end of each variable (DS3, DS5 and DS10), as previously published (de Paiva et al., 2009 (link); de Paiva et al., 2006a (link); de Paiva et al., 2006b (link)). Mice were placed in a cage with a perforated plastic screen on one side to allow airflow from a fan placed six inches in front of it for 16 h/day. Room humidity was maintained at 30–35%. Control mice were maintained in a non-stressed (NS) environment containing 50–75% relative humidity without exposure to forced air. Since dry eye is more prevalent in women and male mice do not respond well to desiccation, only female mice were used (Gao et al., 2015 (link); Schaumberg et al., 2003 (link), 2009 (link)).
6
Comprehensive Neuroinflammation Measurement
7
Chitosan Oligosaccharide Effects on Bone Microstructure
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Six-week-old male C57BL/6 mice were from Shanghai West Poole-Baykay Laboratory Animal Co., Ltd. (Shanghai, China); bacterial LPS and a tartrate-resistant acid phosphatase (TRAP) detection kit were purchased from Sigma-Aldrich (St. Louis, MO, USA); chitosan oligosaccharide powder (polymerization degree, 2–10; average molecular weight, 1000 Da; purity, > 98%; deacetylation degree, 99%). The micro-CT scanner (μCT-100) used in this study was purchased from Scanco Medical AG, Brüttisellen, Switzerland. This study was approved by the Ethics Committee of Shanghai Ninth People’s Hospital (SH9H-2019-A502–1).
8
High-Salt Diet and Angiotensin II-Induced Hypertension
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Male Dahl salt-sensitive rats (DSS: SS/JrHsdMcwiCrl) and C57BL-6 wild-type mice aged 8-10 weeks were obtained from Vital River Laboratory Animal Technology (Beijing). In total, 12 rats were initially fed the AIN-76A diet with 0.4% NaCl (low salt [LS], cat. 113755; Dyets) for 2 weeks. Then, six 12-week-old rats were randomly switched to 8% NaCl diet (high salt [HS]; cat. 100078; Dyets) for 8 weeks [36 (link)]. The remaining LS rats were used as controls. C57BL6 mice aged 12 weeks were subcutaneously infused with Ang II (n=9, 1.44 mg/kg/day, Sigma) for the hypertension model or sterile saline for controls (n=10) by using osmotic minipump for 4 weeks [37 (link)]. All animals were housed in a temperature-controlled room with a 12-h light/dark cycle and free access to tap water. Overdose of pentobarbital sodium (200 mg/kg, iv) was used for euthanasia at the end of the experiment. The trial was performed in a double-blinded and randomized fashion. The experimental protocols followed the Guide for the Care and Use of Laboratory Animals of Peking Union Medical College Hospital.
9
Angiogenic Potential of VEGF-A
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All the procedures involving mice and their care conformed to institutional guidelines that complied with national and international laws and policies (EEC Council Directive 86/609, OJ L 358, 12 December 1987). Seven-week-old C57BL/6 mice (Charles River Laboratories International, Inc., Wilmington, MA, USA) were injected subcutaneously with 400 µL of 5% alginic acid (Sigma) containing PBS or 500 ng of VEGF-A, in the absence or in the presence of 5% Histogel solution. One week after injection, mice were sacrificed, and plugs were harvested and processed for RT-qPCR as previously described [26 (link)]. The mRNA expression levels of murine CD31 and CD45 were normalized to the levels of human GAPDH. Data are expressed as relative expression ratios (ΔΔCt—fold increase) using one PBS plug as the reference.
10
Desiccating Stress Dry Eye Model in Mice
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