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Facsvantage diva sorter

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The FACSVantage/DiVa sorter is a flow cytometry instrument designed for high-performance cell sorting. It is capable of accurately detecting and sorting cells based on their physical and fluorescent characteristics. The FACSVantage/DiVa sorter is a versatile tool that can be used in a variety of research and clinical applications.

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Lab products found in correlation

Retinoic acid, by Merck Group (4 mentions) Mouse fibroblasts, by Merck Group (3 mentions) Lsrfortessa, by BD (1 mentions) Facscanto, by BD (1 mentions)

Spelling variants of this product

FACSVantage (205 citations) FACSVantage DiVa (18 citations) FACSVantage DiVa cell sorter (6 citations) FACSVantage sorter (5 citations) FACSVantage SE w/DiVa cell sorter (2 citations)

5 protocols using facsvantage diva sorter

1

Differentiation of Mouse Embryonic Stem Cells into Spinal Motor Neurons

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Mouse embryonic stem cells (meSCs) expressing the green fluorescent protein (GFP) under the MN-specific promoter HB9 (HBG3 cells) were a kind gift from Tom Jessell (Columbia University, New York, NY, USA). Their differentiation into MNs was performed as described [19 (link),51 (link)]. Briefly, meSCs cells were cultured on a monolayer of EmbryoMax® Primary Mouse Embryonic Fibroblasts (Millipore, Billerica, MA, USA). For differentiation into MNs, cells were lifted with trypsin and resuspended (around 1–2 × 106 meSCs per 10 cm2 dish) in culture medium consisting of knockout DMEM/F12, 10% knockout serum replacement, 1% N2, 0.5% L-glutamine, 0.5% glucose (30% in water), and 0.0016% 2-mercaptoethanol) on nonadherent Petri dishes to enable the formation of embryoid bodies (EBs). After one day of recovery, 2 μM retinoic acid (Sigma-Aldrich, St. Louis, MO, USA) and 2 μM smoothened agonist (SAG, a Shh agonist) were added freshly every day with the new medium. After 5 days of differentiation, EBs were dissociated and sorted based on levels of GFP using a FACSVantage/DiVa sorter (BD Biosciences, Rockville, MD, USA). Representative bright field images of meSCs and EBs, as well as immunocytochemical staining for ChAT and Smi-32 MN markers, are depicted in Supplementary Figure S2C.
Gomes C., Sequeira C., Likhite S., Dennys C.N., Kolb S.J., Shaw P.J., Vaz A.R., Kaspar B.K., Meyer K, & Brites D. (2022). Neurotoxic Astrocytes Directly Converted from Sporadic and Familial ALS Patient Fibroblasts Reveal Signature Diversities and miR-146a Theragnostic Potential in Specific Subtypes. Cells, 11(7), 1186.
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2

Differentiation of Motor Neurons from Stem Cells

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Mouse ESCs or iPSCs expressing Hb9∷GFP reporter were cultured on top of inactivated mouse fibroblasts (Millipore). Motor neuron differentiation was induced by plating 1-2 × 106 mES cells per 10 cm dish in the presence of 2 μM retinoic acid (Sigma-Aldrich) and 2 μM purmorphamine (Calbiochem, Billerica, MA). After 5 days of differentiation, embryonic bodies were dissociated and sorted based on levels of GFP using a FACSVantage/DiVa sorter (BD Biosciences, Rockville, MD).
Israelson A., Ditsworth D., Sun S., Song S., Liang J., Hruska-Plochan M., McAlonis-Downes M., Abu-Hamad S., Zoltsman G., Shani T., Maldonado M., Bui A., Navarro M., Zhou H., Marsala M., Kaspar B.K., Da Cruz S, & Cleveland D.W. (2015). Macrophage Migration Inhibitory Factor (MIF) as a Chaperone Inhibiting Accumulation of Misfolded SOD1. Neuron, 86(1), 218-232.
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3

Myh6 Promoter-Driven Cell Sorting

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Cells were dissociated to single cell suspensions and analyzed on a FACSCanto or LSRFortessa (BD Biosciences) for eGFP under control of the Myh6 promoter. For extracellular staining, cells were incubated for 30 minutes with antibodies as listed in the supplement. Prior to analysis, cells were incubated with propidium iodide (PI) to label non-viable cells. Data analysis was performed using FlowJo (Treestar). Measured events were gated for PI negative populations (exclusion of dead cells) and/or forward/side scatter (exclusion of debris and aggregates) before producing histograms or dot plots. Samples for sorting where processed similarly and were run on a FACS Vantage-Diva sorter (BD Biosciences).
Cabral-Teixeira J., Martinez-Fernandez A., Cai W., Terzic A., Mercola M, & Willems E. (2015). Cholesterol-derived glucocorticoids control early fate specification in embryonic stem cells. Stem cell research, 15(1), 88-95.
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4

Directed Differentiation of Hb9-Expressing Motor Neurons

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Mouse ESCs or iPSCs expressing Hb9::GFP reporter were cultured on top of inactivated mouse fibroblasts (Millipore). MN differentiation was induced by plating 1-2 × 106 cells per 10 cm dish in the presence of 2 μM retinoic acid (Sigma-Aldrich) and 2 μM purmorphamine (Calbiochem, Billerica, MA). After 5 days of differentiation, embryonic bodies were dissociated and sorted based on levels of GFP using a FACSVantage/DiVa sorter (BD Biosciences, Rockville, MD).
Song S., Miranda C.J., Braun L., Meyer K., Frakes A.E., Ferraiuolo L., Likhite S., Bevan A.K., Foust K.D., McConnell M.J., Walker C.M, & Kaspar B.K. (2016). MHC class I protects motor neurons from astrocyte-induced toxicity in amyotrophic lateral sclerosis (ALS). Nature medicine, 22(4), 397-403.
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5

Directed Differentiation of Hb9-Expressing Motor Neurons

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Mouse ESCs or iPSCs expressing Hb9::GFP reporter were cultured on top of inactivated mouse fibroblasts (Millipore). MN differentiation was induced by plating 1-2 × 106 cells per 10 cm dish in the presence of 2 μM retinoic acid (Sigma-Aldrich) and 2 μM purmorphamine (Calbiochem, Billerica, MA). After 5 days of differentiation, embryonic bodies were dissociated and sorted based on levels of GFP using a FACSVantage/DiVa sorter (BD Biosciences, Rockville, MD).
Song S., Miranda C.J., Braun L., Meyer K., Frakes A.E., Ferraiuolo L., Likhite S., Bevan A.K., Foust K.D., McConnell M.J., Walker C.M, & Kaspar B.K. (2016). MHC class I protects motor neurons from astrocyte-induced toxicity in amyotrophic lateral sclerosis (ALS). Nature medicine, 22(4), 397-403.
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