Western blot were carried out as previously described [38 (link)]. Immunostaining was carried out using a goat monoclonal antibodies against RhoA (2117), Rock2 (9029), Rac1 (2465), Cdc42 (2466), SDF-1α (3740), integrin (α4-4600; α5-4705; αV-4711; β3-4702; β4-4707; β5-4708), actin (3200) (1/1000, Cell signaling) and a secondary polyclonal mouse anti-goat antibody HRP conjugated (1/2000, cell signaling). Blots were developed using HRP and chemiluminescent peroxidase substrate (#CPS1120, Sigma). Data were collected using Geliance CCD camera (Perkin Elmer), and analyzed using ImageJ software (NIH).
Geliance ccd camera
Manufactured by PerkinElmer
Sourced in United States, Morocco
The Geliance CCD camera is a high-performance imaging device designed for scientific and industrial applications. It features a charge-coupled device (CCD) sensor that captures and converts light into digital signals, allowing for the capture of high-quality images and data. The Geliance camera offers reliable and precise image acquisition capabilities without any additional interpretations or extrapolations.
Automatically generated - may contain errors
Lab products found in correlation
Cps1120, by Merck Group (5 mentions)
Horseradish peroxidase hrp, by Thermo Fisher Scientific (2 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions)
Proteome profiler human cytokine array kit, by R&D Systems (1 mentions)
β catenin antibody sampler kit, by Cell Signaling Technology (1 mentions)
Rho gtpase antibody sampler kit, by Cell Signaling Technology (1 mentions)
8 protocols using geliance ccd camera
1
Comprehensive Protein Expression Analysis
2
Quantitative Western Blot Analysis
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Western blots were conducted as previously described [68 (link)]. Immunostaining was performed using goat monoclonal antibodies against phospho-Akt (S473) (cell signaling #9271) and actin (1/1000, cell signaling) and a secondary polyclonal mouse anti-goat antibody HRP conjugate (1/2000, cell signaling). Blots were developed using HRP and chemiluminescent peroxidase substrate (#CPS1120, Sigma). Data were collected using a Geliance CCD camera (Perkin Elmer, Waltham, MA, USA) and analyzed using ImageJ software (NIH) [21 (link)].
3
Cytokine Array Profiling of Starved Cells
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All cells were starved for 24 h prior the cytokine Array experiment. 200 μg of protein was loaded on RayBio® Human Cytokine Antibody Array G Series 1000 (Raybiotec, Norcross, GA) according to manufacturer’s instructions. Arrays were revealed using HorseRadish Peroxidase (HRP) and SuperSignal West Pico Chemiluminescent Substrate (Thermo-Scientific, Dubai, Emirates). Data were collected using Geliance CCD camera (Perkin Elmer, MA), and extracted using ImageJ software (NIH). Briefly, the pictures of the arrays were inverted and background subtracted. We then defined the area for signal capture for all spots as 110–120 μm diameter, using the same area for every spot. We defined our signal as the median pixel density value. For the comparison, the independent arrays values were normalized on their positive control intensity value.
4
Immunostaining and Western Blot Analysis
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Western blot were carried out as previously described [14 (link)]. Immunostaining was carried out using a goat monoclonal antibody against Phospho PYK2 #3291, PYK2 #3292, IL-6 #2153, actin #3200 (1/1000, Cell signaling) and a secondary polyclonal mouse anti-goat antibody HRP conjugated (1/2000, cell signaling). Blots were developed using HRP and chemiluminescent peroxidase substrate (#CPS1120, Sigma). Data were collected using Geliance CCD camera (Perkin Elmer), and analyzed using ImageJ software (NIH).
5
Phosphorylated Akt Detection via Western Blot
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Western blots were carried out as previously described [16 (link)]. Immunostaining was carried out using a goat monoclonal antibodies against phospho-Akt (S473) (Cell Signaling #9271) and actin (1/1000, Cell signaling) and a secondary polyclonal mouse anti-goat antibody HRP conjugated (1/2000, cell signaling). Blots were developed using HRP and chemiluminescent peroxidase substrate (#CPS1120, Sigma). Data were collected using Geliance CCD camera (Perkin Elmer), and analyzed using ImageJ software (NIH).
6
Angiogenesis and Cytokine Profiling
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200 μg of protein was loaded on Proteome Profiler™ Human Angiogenesis or Proteome Profiler Human Cytokine Array Kit (R&D Systems®) according to manufacturer’s instructions. Arrays were revealed using HorseRadish Peroxidase (HRP) and SuperSignal West Pico Chemiluminescent Substrate (Thermo-Scientific, Dubai, Emirates). Data were collected using Geliance CCD camera (Perkin Elmer, MA), and extracted using ImageJ software (NIH). Briefly, the pictures of the arrays were inverted and background subtracted. We then defined the area for signal capture for all spots as 110–120 micron diameter, using the same area for every spot. We defined our signal as the median pixel density value. For comparison, the independent arrays values were normalized on their positive control intensity value.
7
Western Blot and Immunostaining Analysis
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Western blot were carried out as previously described [7 (link)]. Immunostaining was carried out using a rabbit monoclonal caspase3, caspase9, actin and PhosphoAKT antibody (1/1000, Cells signaling) and a secondary polyclonal mouse anti-rabbit antibody HRP conjugated (1/2000, cell signalling). Blots were developed using HRP and chemiluminescent peroxidase substrate (#CPS1120, Sigma). Data were collected using Geliance CCD camera (Perkin Elmer), and analyzed using ImageJ software (NIH).
8
Integrin and Wnt/β-Catenin Signaling Pathway Analysis
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Western blot was carried out as previously described [80 (link)]. Immunostaining was carried out using a goat monoclonal antibodies against Integrins: α1, α5, α6, αV and β3 (#ab34445, #ab150361, #ab20142, #ab179475, #ab75872, abcam) and Wnt/β-Catenin Activated Targets Antibody Sampler Kit (Cell Signaling # 8655S), β-Catenin Antibody Sampler Kit (Cell Signaling #2951S), Rho-GTPase Antibody Sampler Kit(Cell Signaling, #9968S), actin (1/1000, Cell signaling) and a secondary polyclonal mouse anti-goat antibody HRP conjugated (1/2000, cell signaling). Blots were developed using HRP and chemiluminescent peroxidase substrate (#CPS1120, Sigma). Data were collected using Geliance CCD camera (Perkin Elmer), and analyzed using ImageJ software (NIH).
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