Anti-GABABR1 and anti-GABABR2 mAbs were purchased from Abcam (Cambridge, UK). N-cadherin, E-cadherin, Vimentin and Hippo/YAP1 pathway antibodies were purchased from CST (Danvers, MA, USA). An anti-actin mAb, an anti-GAPDH mAb and an anti-histone H3 were purchased from Proteintech (Chicago, USA).
Anti gapdh mab
Manufactured by Proteintech
Sourced in United States
Anti-GAPDH mAb is a monoclonal antibody that specifically recognizes the GAPDH protein. GAPDH is a common housekeeping gene that is widely used as a loading control in western blotting and other applications.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylidene difluoride membrane, by Merck Group (2 mentions)
Anti histone h3, by Proteintech (1 mentions)
Horseradish peroxidase hrp conjugated goat anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Bca protein assay kit, by Wanlei (1 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)
Foxc1, by Abcam (1 mentions)
Hrp conjugated secondary antibody, by Proteintech (1 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
Glyburide, by Merck Group (1 mentions)
Gallein, by Bio-Techne (1 mentions)
Monosodium urate msu, by Thermo Fisher Scientific (1 mentions)
Bay11 7082, by Merck Group (1 mentions)
Lps b5 ultrapure, by InvivoGen (1 mentions)
Sb203580, by Cayman Chemical (1 mentions)
Fla st, by InvivoGen (1 mentions)
Anti β actin mab, by BD (1 mentions)
Muramyl dipeptide mdp, by InvivoGen (1 mentions)
Anti asc al 177, by Adipogen (1 mentions)
Sp600125, by Merck Group (1 mentions)
Bapta am, by Merck Group (1 mentions)
4 protocols using anti gapdh mab
1
Antibody Procurement for Cell Analysis
2
Analyzing H60 Expression in EMT-6 Cells
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The EMT-6 cells were incubated with or without CpG ODN-conditioned supernatant for 24 hours, washed with PBS, harvested and lysed in ice-cold RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.4, 1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS). The cell lysate were centrifuged (14,500g for 20 min at 4°C) and the supernatant was collected, quantified using a BCA protein assay kit (Wanleibio, Shenyang, China) and separated by 12% SDS-PAGE. Then the separated protein bands were transferred to polyvinylidene difluoride membranes (Millipore, USA). The membranes were blocked overnight at 4°C with Tris-buffered saline containing 5% nonfat dried milk and then incubated with anti-H60 mAb (R&D system, USA) or anti-GAPDH mAb (Proteintech, USA). Blots were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG for 1 h at room temperature (Jackson immunoresearch laboratories, USA). Immunoreactive bands were visualized with SuperSignal West Pico chemiluminescent substrate (Thermo, USA).
3
Western Blot Analysis of FOXC1 and LOX
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Total proteins were loaded on a 10% SDS‐PAGE gel and transferred onto a polyvinylidene difluoride membrane (Millipore). Subsequently, the membrane was blocked by TBST containing 5% non–fat milk for 1 hour and incubated overnight at 4°C with the primary antibodies against FOXC1 (1:1000; Abcam), LOX (1:1000; Abcam) and anti–GAPDH mAb (1:5000; Proteintech) as an internal control. Afterwards, the blots were labeled for 1 h with HRP‐conjugated secondary antibody (1:10 000; Proteintech). Finally, the blots were exposed to the ChemiDoc XRS + system (Bio‐Rad).
4
Biochemical Inhibition of NLRP3 Inflammasome
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All chemicals and reagents were purchased from Sigma (St. Louis, MO, USA) and Invitrogen (CA, USA) unless specified otherwise. ATP, N-acetyl-L-cysteine (NAC), diphenyleneiodonium chloride (DPI), U73122, BAPTA-AM, Glyburide, Bay 11-7082, and sp600125 were all from Sigma. Monosodium urate (MSU) was from Invitrogen. Gallein was from Tocris Bioscience (Bristol UK). U0126 was from Promega (Madison, WI, USA). SB203580 was from Cayman Chemical (Ann Arbor, MI, USA). z-YVAD-fmk (ALX-260-074) and Ac-YVAD-CHO (ALX-260-027) were from Enzo Life Sciences (Farmingdale, NY, USA). Muramyl dipeptide (MDP), Pam3CSK4, FLA-ST, and LPS-B5 Ultrapure were obtained from InvivoGen (San Diego, CA, USA). AZD9056 was from MedChemExpress (Princeton, NJ, USA). Monoclonal antibodies (mAbs) used for Western blotting included: anti-caspase-1 (D3U3E) and anti-human IL-1β (D7F10) were obtained from Cell Signaling Technology (Beverly, MA, USA); anti-ASC (AL177) was from AdipoGen (San Diego, CA, USA); anti-NLRP3 (ALX-804-819) was from Enzo Life Sciences; Anti-β-actin mAb was purchased from BD Biosciences (San Jose, CA, USA). Anti-GAPDH mAb was from Proteintech (IL, USA). Abs used for cell stimulation (2A1 and mouse IgG1 control) and for the detection of signaling molecules have been described previously (24 (link)).
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