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Balb c nu nu mice

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BALB/c nu/nu mice are a type of immunodeficient mouse model. These mice lack a functional thymus and are unable to produce mature T cells, resulting in a severely compromised immune system. The BALB/c nu/nu mice are commonly used in research applications that require an animal model with a lack of cell-mediated immunity.

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Lab products found in correlation

Matrigel, by BD (10 mentions) Matrigel, by Corning (5 mentions) Balb c, by Charles River Laboratories (3 mentions) C57bl 6 mice, by Charles River Laboratories (3 mentions) Balb c mice, by Charles River Laboratories (3 mentions) Matrigel matrix, by Corning (2 mentions) Cobra auto gamma, by Hewlett-Packard (2 mentions) Doxil, by Johnson & Johnson (2 mentions) Powerup sybr green master mix, by Thermo Fisher Scientific (2 mentions) Matrigel matrix, by BD (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) C57bl 6 wt, by Charles River Laboratories (1 mentions) C57bl 6j, by Charles River Laboratories (1 mentions) Ct26 cells, by American Type Culture Collection (1 mentions) Dld 1 cells, by American Type Culture Collection (1 mentions) Minilys, by Bertin Technologies (1 mentions) Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions) 17β estradiol, by Innovative Research (1 mentions) Scid beige mice, by Charles River Laboratories (1 mentions) C57bl 6, by Charles River Laboratories (1 mentions)

163 protocols using balb c nu nu mice

1

Evaluating Anticancer Drugs in PDX Models

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F2 generation PDXs obtained from NOD-SCID mice were engrafted into BALB/c-nu/nu mice (Charles River Laboratories, Inc.), and small fragments were retransplanted into new BALB/c-nu/nu mice for drug administration tests. When tumours were palpable, F3 generation PDX tumour-bearing BALB/c-nu/nu mice were randomized to treatment or control groups consisting of six mice each. BALB/c-nu/nu mice were chosen because passaged tumours would continue to grow in less immunocompromised mouse strains and to ensure the comparability of results because the appropriate doses were previously assessed in this strain by our group. PDX tumour-bearing BALB/c-nu/nu mice were treated for two consecutive weeks with weekly paclitaxel (20 mg/kg; Nippon Kayaku Co., Ltd.), weekly cisplatin (2.5 mg/kg; Nippon Kayaku), or weekly PBS (as a control) intravenous injections. Two-dimensional tumour measurements were performed with a sliding calliper once weekly. Individual tumour volumes were calculated using the formula: V (mm3) = 1/2×axb2 (where ‘a’ was the longest tumour diameter and ‘b’ was the shortest tumour diameter). We could test drug response of P-2, P-3 and P-5 in vivo, but not of P-1 and P-4, because we only had frozen PDX specimens of P-1 and P-4 and could not make PDX models for this examination from these samples.
Makita H., Endo K., Kasahara Y., Nakata A., Moriyama-Kita M., Ishikawa K., Ueno T., Nakanishi Y., Kondo S., Wakisaka N., Gotoh N, & Yoshizaki T. (2021). Xenografts derived from patients with head and neck cancer recapitulate patient tumour properties. Oncology Letters, 21(5), 385.
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2

Xenograft Tumor Model in Mice

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All animal experiments were performed in accordance with the United Kingdom Home Office Animal (scientific procedures) Act 1986. 2 × 106 SKOV3-ip1 and 4 × 106 and SKOV3-TRip2 cells were injected subcutaneously into female Balb/c nu/nu mice aged 6–9 weeks (Charles River Laboratories). Tumour dimensions were measured using an electronic calliper and the volume calculated using the following equation: volume = ((π/6) × h × w × l), where h, w and l represent, height, width and length, respectively. Imaging studies took place when tumour size reached approximately 150–250 mm3.
Pereira R., Flaherty R.L., Edwards R.S., Greenwood H.E., Shuhendler A.J, & Witney T.H. (2022). A prodrug strategy for the in vivo imaging of aldehyde dehydrogenase activity. RSC Chemical Biology, 3(5), 561-570.
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3

Aging Mice Identification Protocol

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Four-week-old male Balb/c nu/nu mice and 8-week-old male C57BL/6 mice were purchased from Charles River (Wilmington, MA, United States). The mice were housed in a specific pathogen-free (SPF) environment with free access to food and water. The naturally aged male mice were fed on a normal diet for at least 24 months and then were used for identification of p16INK4A staining and SA-gal staining. All experiments involving the handling of mice were approved by the animal ethics committee of the First Affiliated Hospital of Hunan Normal University. The human tissue samples were obtained with patients’ informed consent, and animal experiments and research on human tissues were approved by the Clinical Research Ethics Committee of the First Affiliated Hospital of Hunan Normal University.
Yu A.Q., Wang J., Jiang S.T., Yuan L.Q., Ma H.Y., Hu Y.M., Han X.M., Tan L.M, & Wang Z.X. (2021). SIRT7-Induced PHF5A Decrotonylation Regulates Aging Progress Through Alternative Splicing-Mediated Downregulation of CDK2. Frontiers in Cell and Developmental Biology, 9, 710479.
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4

Xenograft Tumor Model in Mice

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SH-SY5Y cells (5×106 cells) were mixed 1:1 with Matrigel (Corning, New York, NY, USA) and injected subcutaneously into the flanks of total 30 female BALB/c nu/nu mice (5–6 weeks old) purchased from Charles River Laboratories (Yokohama, Japan). After the tumor size reached 500 mm3, the mice were randomized into two groups and intravenously injected with DMSO or CCC-003 (0.3 mg/kg/week) once a week for 2 weeks (n = 8 in each group). Tumor volume (W×W×L/2) and body weights were measured every 3 or 4 days. Animal health was monitored by body weight and general assessment of animal behavioral activity. Adverse events were judged by body weight change and a humane endpoint was determined by 20% reduction in body weight. None of mice reached the endpoint during the experiments. When necessary, mice were anesthetized with 2% isoflurane. The mice were sacrificed when the tumor volume reached 2,000 mm3 by administration of anesthesia followed by cervical dislocation.
Ota Y., Yoda H., Inoue T., Watanabe T., Shinozaki Y., Takatori A, & Nagase H. (2021). Targeting anaplastic lymphoma kinase (ALK) gene alterations in neuroblastoma by using alkylating pyrrole-imidazole polyamides. PLoS ONE, 16(9), e0257718.
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5

Orthotopic Breast Cancer Xenograft Model

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All animal procedures conducted meet the standards required by the UKCCCR guidelines and were performed under the authority of project and personal licenses issued by the Home Office, U.K., reviewed by the Animal Welfare and Ethical Review Board at the CRUK Cambridge Institute. 300,000 MDA-MB-231 cells in a final volume of 100μL of 1:1 DMEM (GIBCO) and matrigel (BD) were inoculated orthotopically in the mammary fat pad of both flanks of 10 seven-week-old immunodeficient female nude (BALB/c nu/nu) mice (Charles River). All mice were kept with 5R58 diet (PicoLab) in Tecniplast Green Line cages, individually ventilated in 12/12 h ON/OFF light cycles. Tumors were measured externally with Vernier calipers. The probe was injected and the mouse imaged when tumor size was between 0.5 and 1cm diameter. Animals were killed by exsanguination and cervical dislocation as confirmation of death. Tumors, liver, kidney, spleen, heart and brain were collected for ex vivo analysis. Afterwards, tumors and livers were divided into 3 parts for histopathology, microscopy and LC-MS/MS analysis. Kidney, spleen, heart and brain were paraffin embedded. One mouse developed only one tumor that could be fully analyzed and one small tumor, which was not analyzed ex vivo.
Weber J., Bollepalli L., Belenguer A.M., Di Antonio M., De Mitri N., Joseph J., Balasubramanian S., Hunter C.A, & Bohndiek S.E. (2019). An activatable cancer-targeted hydrogen peroxide probe for photoacoustic and fluorescence imaging. Cancer research, 79(20), 5407-5417.
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6

Nude Mouse Anesthesia Protocol

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All procedures in this study were approved by the Institutional Animal Care and Use Committee of the National Institutes of Natural Sciences, Japan; approval numbers are 15A026 and 16A146, and were performed in accordance with the guidelines of the institutional committee for the use of animals for research. All surgeries were performed under isoflurane anesthesia and all efforts were made to minimize suffering. BALB/c-nu/nu mice (females, 4 weeks old) were purchased from Charles River Japan.
Fujikawa A., Sugawara H., Tanaka T., Matsumoto M., Kuboyama K., Suzuki R., Tanga N., Ogata A., Masumura M, & Noda M. (2017). Targeting PTPRZ inhibits stem cell-like properties and tumorigenicity in glioblastoma cells. Scientific Reports, 7, 5609.
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7

Glioblastoma Xenograft Model in Mice

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Six-week-old female BALB/c nu/nu mice (Charles River Japan, Yokohama, Japan) were used in the present study. We inoculated U87MG/Luc/TF cells (1 × 105 cells) suspended in 3 µl of PBS into the right cerebral hemisphere of mice as previously described40 (link). The study was approved by the Committees for Animal Experimentation of the National Cancer Center and National Institute of Radiological Sciences, Japan. All animal procedures were performed in compliance with the Guidelines for the Care and Use of Experimental Animals established by the Committees. These guidelines meet the ethical standards required by law and also comply with the guidelines for the use of experimental animals in Japan.
Takashima H., Tsuji A.B., Saga T., Yasunaga M., Koga Y., Kuroda J.I., Yano S., Kuratsu J.I, & Matsumura Y. (2017). Molecular imaging using an anti-human tissue factor monoclonal antibody in an orthotopic glioma xenograft model. Scientific Reports, 7, 12341.
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8

Knockout Mice for Glycosylation Studies

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Animal study is approved by the Animal Research Committee of Chiba University. GlcNAc6ST-1/-2 DKO14 (link) and FucT-IV/-VII DKO mice7 (link) were maintained as previously described. C57BL/6 WT, BALB/c, and BALB/c nu/nu mice were purchased from Charles River Laboratories (Yokohama, Japan). Mice were treated in accordance with the guidelines of the Animal Research Committee of Chiba University. The reporting in the manuscript follows the recommendations in the ARRIVE (Animals in Research: Report-ing In Vivo Experiments) guidelines46 (link).
Liu W., Xiong W., Liu W., Hirakawa J, & Kawashima H. (2023). A novel monoclonal antibody against 6-sulfo sialyl Lewis x glycans attenuates murine allergic rhinitis by suppressing Th2 immune responses. Scientific Reports, 13, 15740.
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9

Xenograft Tumor Imaging and TRAIL Agonist Treatment

Cited 1 time
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Animal experiments were performed in compliance with a project licence
issued under the Animals (Scientific Procedures) Act of 1986. Protocols were
approved by the Cancer Research UK, Cambridge Institute Animal Welfare and
Ethical Review Body.
Colo205 or MDA-MB-231 cells (5 × 106) were resuspended
in 0.2 mL PBS or a 50:50 mix of Matrigel (Corning, NY, USA) and complete DMEM,
respectively, and implanted subcutaneously in the flanks of female BALB/c
nu/nu mice (Charles River, Wilmington, MA). Tumors were
imaged when they reached ~0.8 cm3. For imaging mice were
fasted for 6 – 8 h (19 (link)) and kept
in a warmed chamber (32ºC) for 1 h prior to induction of anesthesia using
1 – 2.5 % isoflurane (Isoflo, Abbotts Laboratories Ltd, Maidenhead, UK)
in a 50:50 mix of air (1 L/min) and oxygen (1 L/min). MEDI3039, a TRAIL agonist,
(Medimmune, Cambridge, UK) was administered intravenously (i.v.) at 0.4 mg/kg
(17 ,20 (link)).
Hesketh R.L., Wang J., Wright A.J., Lewis D.Y., Denton A.E., Grenfell R., Miller J.L., Bielik R., Gehrung M., Fala M., Ros S., Xie B., Hu D.E, & Brindle K.M. (2019). Magnetic resonance imaging is more sensitive than PET for detecting treatment-induced cell death-dependent changes in glycolysis. Cancer research, 79(14), 3557-3569.
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10

Subcutaneous Xenograft Model of Lung Cancer

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All animal experiments were performed in the U.K. in accordance with the United Kingdom Home Office Animal (scientific procedures) Act 1986, and in accordance with the Guidelines for Care and Use of Laboratory Animals of King's College London. All experiments were approved by the Animal Ethics Committee of “KCL – Waterloo Campus”.
A549 (5 × 106) human lung cancer cells in Dulbecco's PBS (100 μl) were injected subcutaneously into the flank of female Balb/c nu/nu mice aged 6–9 weeks (Charles rivers Laboratories). Tumour growth was monitored daily using an electronic calliper and the volume was calculated using the equation below, whereh, w, and l represent, height, width, and length, respectively.
Brito B., Ruggiero M.R., Price T.W., da Costa Silva M., Genicio N., Wilson A.J., Tyurina O., Rosecker V., Eykyn T.R., Bañobre-López M., Stasiuk G.J, & Gallo J. (2023). Redox double-switch cancer theranostics through Pt(iv) functionalised manganese dioxide nanostructures. Nanoscale, 15(25), 10763-10775.
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