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G csf

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G-CSF is a granulocyte colony-stimulating factor, a type of cytokine protein that stimulates the production and release of neutrophils, a type of white blood cell. It plays a crucial role in the regulation of the immune system and the body's response to infection.

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Lab products found in correlation

Interleukin 3 (il 3), by Thermo Fisher Scientific (52 mentions) Stem cell factor (scf), by Thermo Fisher Scientific (45 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (40 mentions) Gm csf, by Thermo Fisher Scientific (40 mentions) Flt3l, by Thermo Fisher Scientific (27 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (9 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (8 mentions) M csf, by Thermo Fisher Scientific (8 mentions) L glutamine, by Thermo Fisher Scientific (8 mentions) Flt3 ligand, by Thermo Fisher Scientific (7 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions) Glutamax, by Thermo Fisher Scientific (7 mentions) Cxcl1, by Thermo Fisher Scientific (6 mentions) Ifn γ, by Thermo Fisher Scientific (5 mentions) Tnf α, by Thermo Fisher Scientific (5 mentions) Stemspan, by STEMCELL (5 mentions) Dup 697, by Abcam (5 mentions) Il 15, by Sino Biological (5 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (5 mentions) Methocult h4435, by STEMCELL (4 mentions)

Close spelling variants of this product

Recombinant murine G-CSF (9 citations) Recombinant G-CSF (7 citations) Murine G-CSF (6 citations) Recombinant mouse G-CSF (4 citations) Anti-G-CSF antibody (4 citations) Human G-CSF (3 citations) Human G-CSF Instant ELISA Kit (2 citations) G-CSF Recombinant Human Protein (2 citations) Biotinylated polyclonal rabbit anti-G-CSF (2 citations) Mouse G-CSF (2 citations)

149 protocols using g csf

1

Neutrophil Differentiation from CD34+ Cells

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CB CD34+ cells were cultured in IMDM (ThermoFisher, cat. # 12440-053) + 20% FBS (Sigma, cat. # F0926) and 100 ng/mL G-CSF (Peprotech) for 12 days. Media was changed every 3–4 days. iPSC-MDS HPCs were cultured without doxycycline for 4 days in StemSpan SFEM + 50 ng/mL SCF, 50 ng/mL FLT3, 50 ng/mL TPO, 50 ng/mL IL-6, and 10 ng/mL IL-3. Cells were then transferred to IMDM + 20% FBS (Sigma) and 100 ng/mL G-CSF (Peprotech) for 6 days, with one media change at day 3. At the end of differentiation, neutrophils were labeled with CD66b-PE (BD, cat. # 561650) by incubating with the antibody for 10 min at RT, and neutrophils purified using the MACS CD66b+ selection column following kit protocol (Miltenyi, cat. # 130–048-801). Neutrophils were spun onto microscope slides (VWR, cat. # 48311–601) and stained with May-Grunwald and Giemsa stains.
Reilly A., Creamer J.P., Stewart S., Stolla M.C., Wang Y., Du J., Wellington R., Busch S., Estey E.H., Becker P.S., Fang M., Keel S.B., Abkowitz J.L., Soma L.A., Ma J., Duan Z, & Doulatov S. (2022). Lamin B1 deletion in myeloid neoplasms causes nuclear anomaly and altered hematopoietic stem cell function. Cell stem cell, 29(4), 577-592.e8.
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2

Autophagic Analysis of Human Blood Samples

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The clinical records in human blood test were provided by the Department of Orthopaedics, the Second Affiliated Hospital of Soochow University. Human blood samples for autophagic analysis were obtained from volunteer donors with their consent. All experiments with human samples and clinical records were approved by the Research Management Office of the Affiliated Hospital of Soochow University. Human mononuclear cells were enriched by equilibrium centrifugation over a cushion of Ficoll‐Paque Plus (17544652, GE Healthcare) by density gradient centrifugation. Human HSPCs were purified from bone marrow CD45‐positive cells by human CD34‐positive selection kit (18056, STEMCELL Technologies). Human HSPCs were cultured in IMDM (SH30228, GE Healthcare) with 10% FBS, 1% penicillin/streptomycin, SCF (100 ng/ml), Flt3L (100 ng/ml), IL6 (20 ng/ml), IL3 (20 ng/ml), and G‐CSF (20 ng/ml) (PeproTech).
Fang Y., An N., Zhu L., Gu Y., Qian J., Jiang G., Zhao R., Wei W., Xu L., Zhang G., Yao X., Yuan N., Zhang S., Zhao Y, & Wang J. (2020). Autophagy‐Sirt3 axis decelerates hematopoietic aging. Aging Cell, 19(10), e13232.
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3

Cytokine Treatment of Human PBMCs

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25 types of recombinant human cytokines (IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-15, IL-17A, IL-17F, IL-21, IL-22, IL-23, SCF, M-CSF, GM-CSF, G-CSF, TNF-α, IFN-α, IFN-β, IFN-γ, and IFN-λ) were purchased from PeproTech, Inc. (USA). All the lyophilized powder was reconstituted in double distilled water, sodium phosphate, acetic acid or phosphate buffer solution according to the protocol described and then diluted with PBS comprising 5% of trehalose and sub-packaged as 10 μl per tube for further use. All the dilution buffers were purchased from Multi sciences, China.
PBMCs from healthy donors were thawed and then rested overnight at 37°C. Samples were seeded as 1 × 105 cell/100 μl/well in 96 well-plates. Twenty-five types of cytokines were reconstituted as 10 mg/ml and 1 μl was added into a 200 μl medium per well to construct a 50 ng/ml concentration. PBS comprising 5% of trehalose was used as negative control. Samples were incubated in a humidified CO2 incubator at 37°C for 12 h for further analysis.
Xie Z., Zheng J., Wang Y., Li D., Maermaer T., Li Y., Tu J., Xu Q., Liang H., Cai W, & Shen T. (2019). Deficient IL-2 Produced by Activated CD56+ T Cells Contributes to Impaired NK Cell-Mediated ADCC Function in Chronic HIV-1 Infection. Frontiers in Immunology, 10, 1647.
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4

Culture of Human and Mouse Hematopoietic Stem Cells

Cited 2 times
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Human CD34+ cells were cultured in Stemspan serum-free medium II (SFEM II, StemCell Technologies), supplemented with granulocyte-macrophage colony-stimulating factor (GM-CSF) 200pg/mL, leukemia inhibitory factor (LIF) 50pg/mL, granulocyte colony-stimulating factor (G-CSF) 1ng/mL, stem cell factor (SCF) 200pg/mL, macrophage-inflammatory protein-1α (MIP-1α) 200pg/mL, and interleukin-6 (IL-6) 1ng/mL.23 (link)–25 (link) Mouse BM Lineage−Sca-1+c-Kit+ (LSK) cells were cultured in SFEM II supplemented with 10ng/ml SCF and 10ng/ml TPO.25 (link) All cytokines were obtained from Pepro Tech US (Rocky Hill, NJ, USA).
Wang H., Zhao D., Nguyen L.X., Wu H., Li L., Dong D., Troadec E., Zhu Y., Hoang D.H., Stein A.S., Malki M.A., Aldoss I., Lin A., Ghoda L.Y., McDonald T., Pichiorri F., Carlesso N., Kuo Y.H., Zhang B., Jin J, & Marcucci G. (2019). Targeting Cell Membrane HDM2: A Novel Therapeutic Approach for Acute Myeloid Leukemia. Leukemia, 34(1), 75-86.
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5

Culturing Diverse Cell Lines for Research

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293 cells, 293-C7-CRE45 (link) cells were cultured in Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal calf serum (FCS) (HyClone, Logan, UT) 2 mmol/l l-glutamine, and Pen-Strep. Mo7e cells30 (link) were maintained in RPMI 1640 medium containing 10% FCS, 2 mmol/l l-glutamine, Pen-Strep, and granulocyte-macrophage colony stimulating factor (G-CSF) (0.1 ng/ml) (Peprotech, Rocky Hill, NJ). Primary human CD34+-enriched cells from G-CSF mobilized normal donors were obtained from the Fred Hutchinson Cancer Research Center Cell Processing Core Facility. We used PBMC-derived CD34+ cells from two different donors, designated as “donor A” and “donor B.” CD34+ cells were recovered from frozen stocks and incubated overnight in Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 20% FCS, 0.1 mmol/l 2-mercaptoethanol, stem cell factor (50 ng/ml), DNase I (100 µg/ml), 2 mmol/l l-glutamine, Flt3 ligand (Flt3L, 50 ng/ml), interleukin (IL)-3 (10 U/ml), and thrombopoietin (10 ng/ml). Cytokines and growth factors were obtained from Peptotech.
Saydaminova K., Ye X., Wang H., Richter M., Ho M., Chen H., Xu N., Kim J.S., Papapetrou E., Holmes M.C., Gregory P.D., Palmer D., Ng P., Ehrhardt A, & Lieber A. (2015). Efficient genome editing in hematopoietic stem cells with helper-dependent Ad5/35 vectors expressing site-specific endonucleases under microRNA regulation. Molecular Therapy. Methods & Clinical Development, 1, 14057-.
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6

AML Treatment with Entospletinib

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The three patients reported in this manuscript with AML were treated with entospletinib in a clinical trial at Ohio State University. Patient samples used for synergy studies, were obtained from patients treated at the Dana-Farber Cancer Institute after obtaining informed consent under Dana-Farber Cancer Institute Internal Review Board–approved protocols. AML blasts from patients were maintained in SFEM (Stemcell Technologies) with SCF, FLT3L, IL-3, IL-6, and GCSF (PeproTech). All patients provided written informed consent and the studies were conducted in accordance with the Declaration of Helsinki and after Human Research Ethics Committee approval.
Cremer A., Ellegast J.M., Alexe G., Frank E.S., Ross L., Chu S.H., Pikman Y., Robichaud A., Goodale A., Häupl B., Mohr S., Rao A.V., Walker A.R., Blachly J.S., Piccioni F., Armstrong S.A., Byrd J.C., Oellerich T, & Stegmaier K. (2019). Resistance mechanisms to SYK inhibition in acute myeloid leukemia. Cancer discovery, 10(2), 214-231.
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7

Erythroid Colony Forming Assay

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Assays were performed using MethoCult H4100 medium (StemCell Technologies) supplemented with 20% BIT, 50uM β-Mercaptoethanol, 2mM L-glutamine, 100U/mL penicillin/streptomycin, and the human cytokines Erythropoietin (EPO, 6U/mL), Granulocyte Colony Stimulating Factor (G-CSF, 10ng/mL), IL-6(20ng/mL), IL-3 (20ng/mL), and SCF (20ng/mL) (Peprotech). Methocult containing 1×104 cells was dispensed into 35-mm dishes. Colonies of greater than 50 cells were scored at day 14. Colonies were collected and 1×104 cells were used for replating. Collected cells were stained with PE-CD235a (BD Biosciences) for flow cytometry analysis to evaluate erythroid-lineage potential.
Lin S., Wei J., Wunderlich M., Chou F.S, & Mulloy J.C. (2016). Immortalization of human AE pre-leukemia cells by hTERT allows leukemic transformation. Oncotarget, 7(35), 55939-55950.
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8

Functional Characterization of Leukemic Blasts and Residual HSPCs

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Blasts (SSClow, CD45int, CD99high) and residual HSPC (SSClow, CD45int, CD34+, CD38-, CD99low) from primary AML specimens were double FACS–sorted to > 95% purity and seeded in methylcellulose (MethoCult H4435, Stem Cell Technologies). After 14 days, colonies were counted under an Olympus BX41 microscope. For liquid differentiation assays, sorted cells were plated at 1×106/mL in Stem Span (Stem Cell Technologies) media, supplemented with human cytokines that support monocyte/macrophage differentiation: SCF (50 ng/mL), IL-3 (10 ng/mL), Fms-like tyrosine kinase-3–ligand (FLT3-L; 50 ng/mL), G-CSF (10 ng/mL) (Peprotech). After 7 days, immunophenotypic analysis was performed by flow cytometry for CD14 expression.
Cimmino L., Dolgalev I., Wang Y., Yoshimi A., Martin G.H., Wang J., Ng V., Xia B., Witkowski M.T., Mitchell-Flack M., Grillo I., Bakogianni S., Ndiaye-Lobry D., Martín M.T., Guillamot M., Banh R.S., Xu M., Figueroa M.E., Dickins R.A., Abdel-Wahab O., Park C.Y., Tsirigos A., Neel B.G, & Aifantis I. (2017). Restoration of TET2 Function Blocks Aberrant Self-Renewal and Leukemia Progression. Cell, 170(6), 1079-1095.e20.
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9

Expansion of Hematopoietic Stem Cells on MSCs

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1,000 bone marrow CD34+ cells from healthy donor or patients with acute myeloid leukemia (AML) were seeded on 70% confluent MSCs derived from different sources and culture in serum-free medium containing 100 ng/ml each of SCF, Flt3L, TPO and G-CSF (Peprotech, Cat # 30007, 30019, 30018, 30023, Rocky Hill, NJ). Cells were maintained at 37 °C in a humidified atmosphere containing 5% CO2. The co-cultures were incubated for 4–10 days, at which time-periods the progeny of the co-cultured HSPCs was harvested and analyzed (3 ).
Wu L., Lin Q., Ma Z., Chowdhury F.A., Mazumder M.H, & Du W. (2020). Mesenchymal PGD2 activates an ILC2-Treg axis to promote proliferation of normal and malignant HSPCs. Leukemia, 34(11), 3028-3041.
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10

Bone Marrow-Derived Cell Fungicidal Assay

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Bone marrow was isolated as previously described (9 (link)). To obtain BMM and BMDC, we cultured bone marrow cells from uninfected MARCO+/+ or MARCO−/− mice in Dulbecco's minimal essential medium (DMEM, from Life Technologies, Inc., Gaithersburg, MD) containing 20% fetal calf serum, GlutaMAX, MEM-nonessential amino acids, sodium pyruvate, penicillin, and streptomycin. 50 ng/mL G-CSF and 20 ng/mL GM-CSF (PeproTech, Rocky Hill, NJ) were added for BMM and BMDC culture, respectively. Cells were harvested after 7 days of culture. For subsequent experiments, BMM/BMDC were removed from differentiation dishes using cold sterile pyrogen-free PBS and subsequently cultured in RPMI 1640 containing 10% fetal calf serum, GlutaMAX, MEM-nonessential amino acids, sodium pyruvate. To evaluate fungicidal activity of BMDC and BMM, 1 × 105 cells were seeded on 96-well plate and infected with C. neoformans yeast (1 ×104) with or without IFN-γ (100 ng/mL). After 24 hrs incubation, the yeast were collected by lysing BMM/BMDC and enumerated by plating onto Sabouraud dextrose agar plates. Colony values in individual treatment groups were compared to culture wells without BMDC/BMM and expressed as percent of growth inhibition.
Xu J., Flaczyk A., Neal L.M., Fa Z., Eastman A.J., Malachowski A.N., Cheng D., Moore B.B., Curtis J.L., Osterholzer J.J, & Olszewski M.A. (2017). Scavenger receptor MARCO orchestrates early defenses and contributes to fungal containment during cryptococcal infection1. Journal of immunology (Baltimore, Md. : 1950), 198(9), 3548-3557.
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