SA was purchased from Sigma-Aldrich (Merck KGaA; Darmstadt, Germany). Fetal bovine serum (FBS) was purchased from Glico-BRL (Gaithersburg, MD, United States). Recombinant human IL-1β, primary Antibodies specific for iNOS, COX-2, MMP3, ATG7, Beclin-1, p62, LC3I/II, phosphorylated extracellular signal-regulated kinase (p-ERK), ERK, c-Jun N-terminal kinase (JNK), p-JNK, p38, p-p38, P65, and p-P65 were obtained from Cell Signaling Technology Inc. (Beverly, MA, United States) and used at a 1:1,000 dilution. Recombinant rat IL-1β was provided by R&D Systems (United States). Antibodies specific for MMP13 and type II collagen were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, United States) and used at a 1:500 dilution. Antibodies against glyceraldehyde-3-phosphate dehydrogenase (GAPDH), secondary antibodies, and a Cell Counting kit-8 (CCK-8) were obtained from Wuhan Boster Biological Technology Ltd. (Wuhan, China), and the antibodies were used at a 1:500 dilution.
Recombinant human il 1β
Manufactured by Cell Signaling Technology
Sourced in United States
Recombinant human IL-1β is a protein produced in E. coli cells using recombinant DNA technology. It corresponds to the mature form of the human interleukin-1 beta (IL-1β) cytokine.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant rat il 1β, by R&D Systems (1 mentions)
P jnk, by Cell Signaling Technology (1 mentions)
C jun n terminal kinase jnk, by Cell Signaling Technology (1 mentions)
Cell counting kit 8 cck 8, by Wuhan Boster Biological Technology (1 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
Histone h3, by Cell Signaling Technology (1 mentions)
Icam 1, by Cell Signaling Technology (1 mentions)
Vcam 1, by Cell Signaling Technology (1 mentions)
Phospho jnk, by Cell Signaling Technology (1 mentions)
Tnf α, by Cell Signaling Technology (1 mentions)
Phospho s6k, by Cell Signaling Technology (1 mentions)
Phospho erk1 2, by Cell Signaling Technology (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Dmem media, by Corning (1 mentions)
4 protocols using recombinant human il 1β
1
Chondrocyte Inflammation and Autophagy
2
Signaling Pathway Regulation Assay
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Recombinant human IL-1β (#8900) and TNFα (#8902) were purchased from Cell Signaling Technology. Rabbit antibodies against LC3B (#3868), Beclin-1 (#3495), phospho-S6K (#9234), S6K (#2708), ICAM-1 (#4915), VCAM-1 (#13662), phospho-p38 (#4511), p38 (#8690), phospho-ERK1/2 (#9101), ERK1/2 (#9102), phospho-JNK (#4668), JNK (#9252), p65 (#8242), p50 (#3035), histone H3 (#9715), IκBα (#9242), and mouse antibodies against IκBβ (#8635) were purchased from Cell Signaling Technology. Rabbit antibodies against IκBε (ab134143) and IP-10 (ab9807) were purchased from Abcam. Rapamycin (R0395), 3-MA (M9281), Bafilomycin A (B1793), Bay11 (B5556), MG-132 (SML1135), SB202190 (S7067), PD98059 (P215), SP600125 (S5567) and NG25 (SML1332) were purchased from Sigma-Aldrich. BI605906 (#19184) was purchased from Cayman. Horseradish peroxidase-conjugated anti-mouse (sc-2005) and anti-rabbit (sc-2004) IgG were purchased from Santa Cruz.
3
Allicin's Effect on IL-1β-Stimulated Caco-2 Cells
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The Caco-2 cell line was obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were grown at 37°C in 5% CO2 in high glucose DMEM (Gibco-BRC, NY, USA) supplemented with 100 μg/mL streptomycin, 100 IU/mL penicillin (Genom, Zhejiang, China), and 10% fetal bovine serum (FBS) (Gibco-BRC, NY, USA). Cells were seeded at a density of 2 × 105 cells/mL onto six-well tissue culture plates 48 h prior to assay.
After 24 h serum-free starvation, cells were treated with 0.1, 1, or 10 ng/mL of human recombinant IL-1β (Cell Signaling Technology, MA, USA) for 12 h, 24 h, or 48 h. Untreated Caco-2 cells served as control. 10 μg/mL or 25 μg/mL allicin was added with or without the IL-1β stimulation 2 h prior. The culture supernatant was collected and the cells were harvested for the following investigations.
After 24 h serum-free starvation, cells were treated with 0.1, 1, or 10 ng/mL of human recombinant IL-1β (Cell Signaling Technology, MA, USA) for 12 h, 24 h, or 48 h. Untreated Caco-2 cells served as control. 10 μg/mL or 25 μg/mL allicin was added with or without the IL-1β stimulation 2 h prior. The culture supernatant was collected and the cells were harvested for the following investigations.
4
Colon Cancer Cell Lines Maintenance
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CA colon cancer cell lines HT-29 (MSS) and HCT 116 (MSI) were purchased from the American Type Culture Collection (Manassas, VA), while AA colon cancer cell lines, CHTN-06 (MSS) and SB-521 (MSI), were generated at Stony Brook University as previously described (17 (link)). All cell lines were maintained in DMEM media (Corning) supplemented with 10% FBS, 100 U/ml penicillin, 100 U/ml streptomycin, 4.5 g/L glucose and sodium pyruvate, in humidified incubator with 37°C and 5% CO2. Human recombinant IL-1β was purchased from Cell Signaling Technology and resuspended in PBS for a final stock concentration of 10 ug/ml (catalog #8900). 5-FU was purchased from Sigma-Aldrich (catalog #F6627-1G) and resuspended in DMSO (Thermo Fisher Scientific) for a final stock concentration of 380mM. Human recombinant IL-1Ra was purchased from Sigma-Aldrich (catalog ##SRP3084) and resuspended in PBS supplemented with 0.1% BSA for a final stock concentration of 100 ug/ml.
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