Quercetin (purity >95%) was purchased from Acros Organics Company (Fisher Scientific, Pittsburgh, PA, USA). Caprylic/capric triglyceride (Captex® 355) was purchased from Abitec Co. (Janeville, WI, USA). Soy lecithin was purchased from IFC Solutions (Linden, NJ, USA). Polyoxyethylene sorbitan monooleate (Tween® 80) and sodium alginate from algae were purchased from Sigma-Aldrich Chemical Company (St. Louis, MI, USA). All chemical reagents used in this study were of the highest purity commercially available.
Polyoxyethylenesorbitan monooleate tween 80
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom
Polyoxyethylenesorbitan monooleate, also known as Tween 80, is a nonionic surfactant commonly used as an emulsifier and solubilizing agent in various laboratory applications. It is a viscous, colorless to pale yellow liquid with a characteristic odor. Tween 80 is miscible with water and many organic solvents.
Automatically generated - may contain errors
Lab products found in correlation
Sorbitan monooleate span 80, by Merck Group (3 mentions)
Propylene glycol, by Merck Group (2 mentions)
Oleic acid, by Merck Group (2 mentions)
Quercetin, by Thermo Fisher Scientific (1 mentions)
Sodium alginate from algae, by Merck Group (1 mentions)
Sorbitan trioleate span 85, by Merck Group (1 mentions)
Docetaxel, by LC Laboratories (1 mentions)
Lipiodol, by Guerbet (1 mentions)
Disodium hydrogen phosphate, by Merck Group (1 mentions)
Dimethylaminopyridine, by Merck Group (1 mentions)
Dicyclohexylcarbodimide, by Merck Group (1 mentions)
Sodium phosphate, by Merck Group (1 mentions)
Phosphoric acid, by Merck Group (1 mentions)
Sodium nitrite, by Merck Group (1 mentions)
D mannose, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Citric acid, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Ciprofloxacin, by Merck Group (1 mentions)
17 protocols using polyoxyethylenesorbitan monooleate tween 80
1
Formulation and Characterization of Quercetin Lipid Nanoparticles
2
Nanoemulsion for Docetaxel Drug Delivery
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Sorbitan trioleate (Span 85) and polyoxyethylenesorbitan monooleate (Tween 80) were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). Docetaxel was purchased from LC laboratories (Woburn, MA, USA). Lipiodol, an FDA-approved iodized oil, was purchased from Guerbet (Aulnay-Sous-Bois, France). All other materials were of reagent grade and used without further purification.
Oil-in-water emulsions were prepared using a homogenization method as previously described [34 (link)]. Briefly, 7 mg of Docetaxel and 300 μl of Lipiodol were dissolved in ethanol together with varying amounts of surfactant mixture (Tween 80 and Span 85). Ethanol was removed under reduced pressure using a rotary evaporator until a pre-emulsion concentrate was obtained. We then added 0.7 ml of saline dropwise to the pre-emulsion concentrate while vortexing, followed by sonication in a bath sonicator at 37°C for 30 min. The obtained emulsions were placed in an ice bath, then homogenized at 11,000 rpm using a homogenizer (IKA-Wereke, Stufen, Germany) in four intervals (durations: 10, 5, 5, and 5 min), to obtain a homogeneous nanoemulsion. Unloaded Docetaxel was removed from the emulsion dispersion by filtering through an 0.8-μm syringe membrane filter. The filtered emulsions were stored at 4°C until use.
Oil-in-water emulsions were prepared using a homogenization method as previously described [34 (link)]. Briefly, 7 mg of Docetaxel and 300 μl of Lipiodol were dissolved in ethanol together with varying amounts of surfactant mixture (Tween 80 and Span 85). Ethanol was removed under reduced pressure using a rotary evaporator until a pre-emulsion concentrate was obtained. We then added 0.7 ml of saline dropwise to the pre-emulsion concentrate while vortexing, followed by sonication in a bath sonicator at 37°C for 30 min. The obtained emulsions were placed in an ice bath, then homogenized at 11,000 rpm using a homogenizer (IKA-Wereke, Stufen, Germany) in four intervals (durations: 10, 5, 5, and 5 min), to obtain a homogeneous nanoemulsion. Unloaded Docetaxel was removed from the emulsion dispersion by filtering through an 0.8-μm syringe membrane filter. The filtered emulsions were stored at 4°C until use.
3
Diluting Marmoset α1-PI for Analysis
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A 250 mg/l solution of purified marmoset α1-PI was diluted to concentrations of 625, 208.3, 69.4, 23.1, 7.7, 2.6, and 0.0 μg/l, using PBS containing 0.1% polyoxyethylene sorbitan monooleate (Tween 80, Sigma–Aldrich Co. LLC, St. Louis, MO).
4
Synthesis and Characterization of Resveratrol Derivatives
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The solvents used: acetone, ethanol, methanol, n-hexane, chloroform, 2-propanol, and ethyl ether were purchased from Carlo Erba Reagenti (Milan, Italy). Chloroform was purified by standard procedures. Resveratrol or 3,5,4’-trihydroxy-trans-stilbene (MM = 22,825 g/mol), D-Mannose (MM = 180,156 g/mol), ciprofloxacin (MM = 331,346), methacrylic acid (MAA), 85% formic acid (FA), 85% phosphoric acid (AP), sodium nitrite (SN), dicyclohexylcarbodimide (DCC), dimethylaminopyridine (DMAP), dimethyl sulfoxide (DMSO), potassium chloride (KCl), hydrochloric acid (HCl), sodium phosphate (Na3PO4), citric acid, disodium hydrogen phosphate (Na2HPO4), sorbitan monooleate (Span 80) and polyoxyethylene sorbitan monooleate (Tween 80), N,N′-ethylene bis-acrylamide (EBA) were purchased from Sigma-Aldrich (Sigma Chemical CO, St. Louis, MO, USA).
5
Formulation and Evaluation of ATR
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ATR (CAS number 134523-03-8) was kindly supplied by Egyptian International Pharmaceutical Industry Company, Egypt. Oleic acid (99.99%), polyoxyethylene sorbitan monooleate (Tween 80), propylene glycol 99.5%, poloxamer 407, maltose, and other solvents were obtained from Sigma-Aldrich® (St Louis, MO, USA).
6
Nanoparticle Synthesis and Characterization
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Poly(ethylene glycol) methyl ether-block-poly(lactide-co-glycolide) (mPEG-b-PLGA, PEG average Mn 5000, PLGA Mn 55,000), polyoxyethylene sorbitan monooleate (Tween® 80) and RhB were purchased from Sigma Aldrich (Milan, Italy). All other chemical reagents used solvents and deionized water were of analytical grade. Ultrapure water was used throughout this study.
7
Production and Cultivation of M. anisopliae
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Conidia of M. anisopliae ESALQ4676 were obtained by growing agar chunks from the monosporic stock culture in Petri dishes containing potato dextrose agar in a concentration of 39g per liter of distilled water (PDA, Difco, Sparks, MD, USA) for ten days at 26° and 12:12 h photoperiod. Conidia were harvested by washing Petri dishes containing actively sporulating fungal cultures with 10 mL of a sterile aqueous solution with 0.02% polyoxyethylene sorbitan monooleate (Tween 80, Sigma). A conidial suspension of 5×106 conidia mL-1 was used to inoculate 50 mL liquid modified Adamek medium in 250-mL baffled Erlenmeyer flasks giving a final concentration in the culture broth of 5×105 conidia mL-1 (i.e., 10% v/v inoculum). Liquid cultures were incubated at 28° in a rotatory incubator shaker and 350 rpm for 72 hr. Four Erlenmeyer flasks were inoculated and each considered a biological replicate. A volume of 120uL of the 5×106 conidia mL-1 suspension was spread with sterile Drigalski handles in Petri dishes (9 cm diameter) containing solid modified Adamek medium. Four fungal cultures on solid media in separate petri dishes were incubated at 28° for 5 days with a 12:12 h photoperiod, and each considered as one biological replicate.
8
Cytotoxicity Evaluation of Triacetin-based Formulation
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Triacetin (CAS.102-76-1), polyoxyethylene sorbitan monooleate (Tween 80- CAS.9005-65-6), propylene glycol (CAS.57-55-6), sorbitan monooleate (span80-CAS.1338-43-8)), BSA (CAS.9048-46-8), PEG (Mn = 6000 Da) (CAS. 25322683) and MTT reagent were purchased from Sigma Aldrich Company in Germany. Other chemicals and solvents of high purity were purchased from Emertat chimi (Tehran- Iran).
9
Formulation and Characterization of Fullerene-Based Skin Care
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Powdered fullerene (C60) with 99.5% purity, xanthan gum from Xanthomonas campestris, white beeswax, polyoxyethylene sorbitan monooleate (Tween 80), and sorbitan monooleate (Span 80) were purchased from Sigma-Aldrich (St. Louis, USA). The average molecular weight of xanthan gum was estimated as 152 kDa [24 (link)]. Palm kernel oil esters (PKOEs) were synthesized in our laboratory through enzymatic transesterification of palm kernel oil and oleyl alcohol using Lipozyme RM IM as the catalyst [25 ]. The fatty acid composition of PKOEs is shown in Table 1 . Preservative, phenonip, was obtained from Bramble Berry (Bellingham, USA). Deionized water was purified using Milli-Q water system (Billerica, USA).
10
Antioxidant and Chelating Assays
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Thymol, Polyoxyethylene sorbitan mono-oleate (Tween80) (critical micelle concentration of Tween 80 is 0.002 wt%), 2,4,6-tri(2-pyridinyl)-1,3,5-triazine (TPTZ), and 2,2-diphenyl-1-picrylhydrazyl (DPPH), were purchased from Sigma Aldrich (St. Louis, MO, USA). Neocuproine (2,9-dimethyl-1, 10-phenanthroline), copper (II) chloride dihydrate, trichloroacetic acid (TCA), 2-thiobarbituric acid (TBA), and 2-deoxy-D-ribose were purchased from Aladdin-reagent Inc. (Shanghai, China). The other reagents were purchased from Sinopharm Chemical Reagent Co., Ltd., China. All reagents were used without further purification and used water was double-distilled.
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