Hplc grade solvents

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Canada

HPLC grade solvents are high-purity organic solvents designed for use in high-performance liquid chromatography (HPLC) applications. These solvents are meticulously filtered and purified to minimize the presence of impurities and ensure consistent performance in HPLC systems.

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Lab products found in correlation

Formic acid, by Thermo Fisher Scientific (14 mentions) Methanol, by Thermo Fisher Scientific (13 mentions) Acetonitrile, by Thermo Fisher Scientific (8 mentions) Formic acid, by Merck Group (5 mentions) Indomethacin, by Merck Group (4 mentions) Dimethyl sulfoxide (dmso), by Merck Group (4 mentions) Ammonium bicarbonate, by Merck Group (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Gallic acid, by Merck Group (3 mentions) High performance liquid chromatography, by Thermo Fisher Scientific (3 mentions) Methanol, by Merck Group (3 mentions) Glutathione (gsh), by Merck Group (3 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (3 mentions) Iodoacetamide, by Merck Group (3 mentions) Millipore ziptip c18 pipette tips, by Merck Group (3 mentions) Deferoxamine mesylate, by Merck Group (3 mentions) Pentostatin, by Merck Group (3 mentions) Phosphodieasterase 1 and 2, by Merck Group (3 mentions) Dnase 1 and dnase 2, by Merck Group (3 mentions) Nuclease p1 from penicillium citrinum, by Merck Group (3 mentions)

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HPLC grade glass distilled solvents HPLC grade solvents (Water and Acetonitrile HPLC grade water and solvents HPLC-grade organic solvents HPLC solvents HPLC grade

134 protocols using hplc grade solvents

HPLC Analysis of Nucleic Acids

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Mobile phases were prepared with HPLC grade solvents (Fisher Scientific, UK).
Nucleic acids were analysed by IP RP HPLC on a U3000 RSLC UPLC (Thermo Fisher, UK) with the following columns: Accucore C₁₈ 150 mm × 2.1 mm I.D. (2.6 μm superficially porous silica particles 80 Å pore size), Accucore C₁₈ 150 mm × 2.1 mm I.D. (2.6 μm superficially porous silica particles 150 Å pore size). The Accucore columns contain 2.6 μm solid-core particles, with a porous layer of 0.5 μm with an average particle size distribution of 1.12 (D₉₀/D₁₀). Accucore C₄ 150 mm × 4.6 mm I.D. (a research column based on superficially porous silica 4.0 μm superficially porous silica particles 400 Å pore size). Chromatograms were recorded using a VWD detector at 260 nm with a 2.5 μl flow cell. Tubing was 100 μm I.D. for flow rates of 0.4 ml/min and 180 μm I.D. for flow rates of 1.0 ml/min.

Close E.D., Nwokeoji A.O., Milton D., Cook K., Hindocha D.M., Hook E.C., Wood H, & Dickman M.J. (2016). Nucleic acid separations using superficially porous silica particles. Journal of Chromatography. a, 1440, 135-144.

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Colorectal Cancer Cell Antioxidant Assays

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ACS-grade solvents (acetone, methanol, and 95% ethanol), HPLC-grade solvents (water, methanol, and acetonitrile), glass wool, Gibco fetal bovine serum, glacial acetic acid and hydrochloric acid were acquired from the Fisher Scientific Co., LLC (Suwanee, GA, USA). Sephadex LH-20, Amberlite XAD-16, Folin & Ciocalteu’s phenol reagent, gallic acid (>99%), (+)-catechin hydrate (>99%), chlorogenic acid (≥98%), protocatechuic acid (≥97%), vanillic acid (≥97%), fluorescein 3′,6′-dihydroxyspiro[isobenzofuran-1[3H]9′[9[H]xanthen]-3-one), Trolox [(±)-6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, 97%], TPTZ (2,4,6-tripyridyl-S-triazine, ≥99.0%), quercetin-3-O-rutinoside hydrate (≥94%), iron(II) sulfate heptahydrate (≥99.0%), iron(III) chloride hexahydrate (97%), 2′,7′-dichlorofluorescin diacetate (DCFH-DA, ≥97%), and 2,2′-azobis [2-amidinopropane] dihydrochloride (ABAP) were purchased from the Sigma-Aldrich Chemical Company (St. Louis, MO, USA). Cyanidin-3-O-glucoside chloride was obtained from the Indofine Chemical Company, Inc. (Hillsborough, NJ, USA). Advanced DMEM, phosphate-buffered saline (PBS), Hanks’ balanced salt solution (HBSS), penicillin-streptomycin, and trypsin-EDTA were purchased from Life Technologies (Grand Island, NY, USA). Human colorectal adenocarcinoma (Caco-2) cells were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Liao X., Greenspan P, & Pegg R.B. (2021). Examining the Performance of Two Extraction Solvent Systems on Phenolic Constituents from U.S. Southeastern Blackberries. Molecules, 26(13), 4001.

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Laxogenin and Diosgenin Purification

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Laxogenin from Proactive Molecular Research (Alachua, FL, USA) and diosgenin from MP Biomedicals Inc. (Irvine, CA, USA) were purchased, and their purity was confirmed by spectral data (1D- and 2D-NMR, ESI-HRMS). HPLC-grade solvents (acetonitrile and methanol) and formic acid were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Water for the mobile phase was purified using a Milli-Q system (Millipore, Burlington, MA, USA).

Avula B., Bae J.Y., Ahn J., Katragunta K., Wang Y.H., Wang M., Kwon Y., Khan I.A, & Chittiboyina A.G. (2023). 6-Oxofurostane and (iso)Spirostane Types of Saponins in Smilax sieboldii: UHPLC-QToF-MS/MS and GNPS-Molecular Networking Approach for the Rapid Dereplication and Biodistribution of Specialized Metabolites. International Journal of Molecular Sciences, 24(14), 11487.

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HPLC Analysis of Isoflavone Metabolites

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High-performance liquid chromatography (HPLC) grade solvents were purchased from Fisher Scientific (Pittsburgh, PA, USA). Formic acid and ammonium acetate were purchased from Merck (Darmstadt, Germany). Genistein (G6776), hippuric acid (HA, 112003), 3,4-dihydroxypenylacetic acid (DOPAC, 11569), benzoic acid (BA, 242381), and 2-(4-hydroxy phenyl)propionic acid (PA, 91378) were purchase from Sigma-Aldrich (St Louis, MO, USA). Genistein 4′-glucuronide (G-4′G, G349990), genistein 7-glucuronide (G-7G, G350015), genistein 7′-sulfate (G-4′S, G350045), and genistein 7-sulfate-4′-glucuronide (G-7S-4′G, G350050) were purchased from Toronto Research Chemicals (Toronto, Ontario, Canada). Equol 7-sulfate (E-7S, sc-219698) and dehydrogenistein (DHG, sc-498873) were purchased from Santa Cruz (Santa Cruz, CA, USA). 3-Hydroxydaidzein (3′-OHD, 1309) and 3-hydroxygenistein (3′-OHG, CFN99257) were purchased from Extrasynthese (Genay, France) and Chemface (Wuhan, China), respectively.

Lee D.H., Kim M.J., Song E.J., Kim J.H., Ahn J., Nam Y.D., Jang Y.J., Ha T.Y, & Jung C.H. (2017). Nutrikinetic study of genistein metabolites in ovariectomized mice. PLoS ONE, 12(10), e0186320.

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Herbal Medicine Extraction Protocol

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All solvents used for the CPC were of analytical grade and purchased from Daejung Chemical (Gyeonggi-do, Korea). The HPLC grade solvents were obtained from Fisher Scientific (Pittsburgh, PA, USA). Ultrapure water was prepared using the Milli-Q SP water purifying system. The herbal medicines used in the experiment were purchased at Kyungdong Oriental Herbal Market (Seoul, Republic of Korea), and voucher specimens were deposited in the Herbarium of the College of Pharmacy, Hanyang University.

Kim J.H., Jung E.J., Lee Y.J., Kim C.Y, & Jeon J.S. (2021). Application of Linear Gradient Solvent System in Centrifugal Partition Chromatography Facilitating Bioassay-Guided Fractionation of Yongdamsagan-Tang, Traditional Oriental Decoction. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 7552169.

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Detailed Protocols for Sensitive Chemical Synthesis

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All chemicals were purchased from Sigma-Aldrich (St. Louis, MO). HPLC grade solvents were purchased from Fisher Scientific (Nepean, ON, Canada). Reactions were performed using glassware that was oven-dried. Air- and moisture-sensitive liquids and solutions were transferred via syringe or stainless steel cannula. Organic solutions were concentrated under reduced pressure (∼15 Torr) by rotary evaporation. Solvents were purified by passage under 12 psi through activated alumina columns. Detailed procedures for synthesis of substrate probes and reactions for Hammett analysis are provided in the ESI.† DESI-MS studies were performed on a high-resolution mass spectrometer (Thermo Scientific LTQ Orbitrap XL Hybrid Ion Trap-Orbitrap mass spectrometer) using a homebuilt DESI source. The details of DESI-MS experiments are described in the ESI.† The online ESI-MS experiment for real time monitoring of the Cu(i) species was performed using the pressurized infusion method originally described by McIndoe and coworkers.²⁰ See the ESI† for complete experimental details.

Banerjee S., Sathyamoorthi S., Du Bois J, & Zare R.N. (2017). Mechanistic analysis of a copper-catalyzed C–H oxidative cyclization of carboxylic acids †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc02240a Click here for additional data file.. Chemical Science, 8(10), 7003-7008.

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Quantification of Oxysterols by Mass Spectrometry

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25-Hydroxycholesterol (25HC, CAS No. 2140-46-7), 27-hydroxycholesterol (27HC, CAS No. 20380-11-4), 7-ketocholesterol (7KC, CAS No. 566-28-9), 7α-hydroxycholesterol (7αHC, CAS No. 566-26-7), cholesterol-5α,6α-epoxide (αEC, CAS No. 1250-95-9), cholesterol-5β,6β-epoxide (βEC, CAS No. 4025-59-6), and cholestane-3β,5α,6β-triol (CT, CAS No. 1253-84-5) and their deuterated standards (25-Hydroxycholesterol-d₆, 27-hydroxycholesterol-d₆ 7-ketocholesterol-d₇, 7α-hydroxycholesterol-d₇, cholesterol-5α,6α-epoxide-d₇, cholesterol-5β,6β-epoxide-d₇, and cholestane-3ß,5α,6ß-triol-d₇) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Methanol, isopropanol, and 2.6-di-tert-butyl-4-methylphenol (butylated hydroxytoluene) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and HPLC-grade solvents were from Fisher Scientific (Pittsburgh, PA, USA).

Kloudova-Spalenkova A., Ueng Y.F., Wei S., Kopeckova K., Guengerich F.P, & Soucek P. (2019). Plasma oxysterol levels in luminal subtype breast cancer patients are associated with clinical data. The Journal of steroid biochemistry and molecular biology, 197, 105566.

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Extraction and Analysis of Canola Meal Phenolics

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The changes in the phenolic composition of defatted canola meal methanolic fractions extracted by ultrasound-assisted extraction were evaluated by high-performance liquid chromatography (HPLC) as described by Nandasiri et al. [10 (link)]. The major phenolic compound, canolol, was identified using the authentic standard with purity of 98% and a detection limit of 0.001 mg/mL. The calibration curve for each standard compound was obtained from 1.0 to 100 μg/mL (n = 11) concentration range with R² = 0.9994 for Sinapic acid, R² = 0.9999 for canolol, and R² = 0.9996 for sinapine. Sinapic acid (purity > 98%), gallic acid ≥ 98%, HPLC grade solvents and other analytical reagents were purchased from Fisher Scientific Canada Ltd (Ottawa, ON, Canada). Sinapine (purity > 97%) and canolol (purity > 97%) were purchased from ChemFaces® Biochemical Co., Ltd. (Wuhan, Hubei, China). A flow chart for the extraction of defatted canola meal-derived sinapates is shown in Figure 1.

Fadairo O.S., Nandasiri R., Nguyen T., Eskin N.A., Aluko R.E, & Scanlon M.G. (2022). Improved Extraction Efficiency and Antioxidant Activity of Defatted Canola Meal Extract Phenolic Compounds Obtained from Air-Fried Seeds. Antioxidants, 11(12), 2411.

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Glycoprotein Analysis by HPLC

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HPLC grade solvents, including water, acetonitrile, methanol and isopropanol alcohol, were purchased from Fisher Scientific (Pittsburgh, PA). Sodium hydroxide beads (20–40 mesh, 97%), iodomethane, acetic acid, formic acid, dimethyl sulfoxide (DMSO) and borane-ammonia complex were obtained from Sigma-Aldrich (St. Louis, MO). Empty spin columns were purchased from Harvard Apparatus (Holliston, MA). PNGase F and digestion buffer were from New England Biolabs (Ipswich, MA). The glycoprotein samples, including ribonuclease B, fetuin, α acidic glycoprotein and pooled human blood serum, were bought from Sigma-Aldrich (St. Louis, MO). The standard glycan samples were purchased from Chemily Glycoscience (Atlanta, GA).

Zhou S., Huang Y., Dong X., Peng W., Veillon L., Kitagawa D.A., Aquino A.J, & Mechref Y. (2017). Isomeric Separation of Permethylated Glycans by Porous Graphitic Carbon (PGC)-LC-MS/MS at High-Temperatures. Analytical chemistry, 89(12), 6590-6597.

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Eudragit S100 and Budesonide Delivery

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Eudragit S100 (ES100) was from Evonik Industries AG (Essen, Germany). Budesonide (BUD), hexadecyltrimethyl ammonium bromide (CTAB), and poloxamer 188 were purchased from Sigma Aldrich (USA). Hyaluronic acid sodium salt (Mwt 1.4*10⁶ Da) was provided by Euromedex (Strasbourg, France). HPLC grade solvents were from Fisher Scientific (UK). Pepsin and pancreatin were also purchased from Sigma Aldrich (USA). All other chemicals and organic solvents were of analytical grade.

Seoudi S.S., Allam E.A., El-Kamel A.H., Elkafrawy H, & El-Moslemany R.M. (2023). Targeted delivery of budesonide in acetic acid induced colitis: impact on miR-21 and E-cadherin expression. Drug Delivery and Translational Research, 13(11), 2930-2947.

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