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Human il 1β

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The Human IL-1β is a laboratory reagent that can be used to measure the levels of interleukin-1 beta (IL-1β) in human samples. IL-1β is a pro-inflammatory cytokine that plays a key role in the immune response and inflammatory processes. This product can be used in various research and diagnostic applications that require the quantification of IL-1β in biological samples.

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Lab products found in correlation

Mouse il 1β, by Thermo Fisher Scientific (8 mentions) Human tnf α, by Thermo Fisher Scientific (7 mentions) Tnf α, by Thermo Fisher Scientific (5 mentions) Human il 6, by Thermo Fisher Scientific (4 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (3 mentions) Trypsin edta, by Thermo Fisher Scientific (3 mentions) Human il 23, by Thermo Fisher Scientific (2 mentions) Pronase e, by Merck Group (2 mentions) Collagenase p, by Roche (2 mentions) Chondrocyte basal medium, by PromoCell (2 mentions) Chondrocyte growth medium supplementmix, by PromoCell (2 mentions) Human ifn β, by Thermo Fisher Scientific (2 mentions) Mouse ifn β, by R&D Systems (2 mentions) L kynurenin, by Merck Group (2 mentions) Ifn γ, by R&D Systems (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Ifn γ, by Thermo Fisher Scientific (2 mentions) Human tgf β, by Thermo Fisher Scientific (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)

47 protocols using human il 1β

1

Purification of Recombinant Human TNF

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Lidocaine, tetrodotoxin (TTX) (Sigma-Aldrich) and human IL-1β (eBiosciences) were purchased. Recombinant and tag-free human TNF was produced in-house. Following expression in Escherichia coli, TNF was purified using a cation exchange column and endotoxins removed by phase separation with Triton X-114.
Steinberg B.E., Silverman H.A., Robbiati S., Gunasekaran M.K., Tsaava T., Battinelli E., Stiegler A., Bouton C.E., Chavan S.S., Tracey K.J, & Huerta P.T. (2016). Cytokine-specific Neurograms in the Sensory Vagus Nerve. Bioelectronic medicine, 3, 7-17.
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2

Cytokine Release Quantification in THP-1 and PBMCs

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Following gene silencing or drug treatment, THP-1 cells or PBMCs were counted, normalized and divided into 96-well plates prior to treatment. Cells were stimulated with LPS or Pam3CSK4 for up to 6 h. Cytokine release was quantified from clarified culture supernatant using either human IL-1β (eBioscience, 88-7261) or human TNF (eBioscience, 88-7346) ELISA kits according to the manufacturer's protocol. Each experiment was replicated and performed in triplicate.
Bednash J.S., Weathington N., Londino J., Rojas M., Gulick D.L., Fort R., Han S., McKelvey A.C., Chen B.B, & Mallampalli R.K. (2017). Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activity. Nature Communications, 8, 15203.
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3

ELISA Quantification of IL-1β

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IL-1β release was measured by ELISA for mouseIL-1β (R&D Systems) or human IL-1β (eBiosciences) following the manufacturer’s instructions and read in a Synergy Mx plate reader (BioTek). The concentration of IL-1β was estimated using a standard with known concentrations of recombinant IL-1β.
Baroja-Mazo A., Compan V., Martín-Sánchez F., Tapia-Abellán A., Couillin I, & Pelegrín P. (2019). Early endosome autoantigen 1 regulates IL-1β release upon caspase-1 activation independently of gasdermin D membrane permeabilization. Scientific Reports, 9, 5788.
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4

Quantitative Cytokine and Chemokine Assays

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Human IL-1β, human TNF-α, rat TNF-α, mouse IL-1β, and TNF-α (all from eBioscience), human CCL2, mouse CRP (both R&D Systems), and CD154 (Boster Bio) were measured using ELISA.
Portillo J.A., Lopez Corcino Y., Miao Y., Tang J., Sheibani N., Kern T.S., Dubyak G.R, & Subauste C.S. (2016). CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy. Diabetes, 66(2), 483-493.
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5

Cytokine Quantification in Cell Culture and Sera

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Cell culture supernatants and mouse sera were assayed for human IL-1β (eBioscience), human IL-18 (R&D Systems), and mouse IL-1β (eBioscience), respectively. Colon organ culture supernatants were assayed for mouse IL-18 (R&D Systems).
Chung I.C., Yuan S.N., OuYang C.N., Lin H.C., Huang K.Y., Chen Y.J., Chung A.K., Chu C.L., Ojcius D.M., Chang Y.S, & Chen L.C. (2018). Src-family kinase-Cbl axis negatively regulates NLRP3 inflammasome activation. Cell Death & Disease, 9(11), 1109.
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6

Primed THP-1 Cells Inflammasome Activation

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Primed THP-1 cells were treated for 45 min with the indicated inhibitor in Opti-MEM medium (Life Technology) prior to activation by 5 mM ATP, 1 μM nigericin, 500 μg/ml MSU crystals, 5 μg/ml Poly(dA:dT)/LyoVec™ (Invivogen) or 3 μg FLA-PA (transfected with ViaFect) for 6 h (MSU crystals were a kind gift of Dr. A. Scanu, University of Padova). Culture supernatants were tested for the production of IL-1β or caspase-1 by enzyme immunoassay (Human IL-1β, eBioscience, San Diego, CA, USA and Caspase-1, Quantikine; R&D Systems, Minneapolis, MN, USA).
Burger D., Fickentscher C., de Moerloose P, & Brandt K.J. (2016). F-actin dampens NLRP3 inflammasome activity via Flightless-I and LRRFIP2. Scientific Reports, 6, 29834.
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7

Cytokine Concentration Measurement

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Supernatants were used to measure concentrations of human IL-1β and human TNF-α (eBioscience).
Portillo J.A., Lopez Corcino Y., Dubyak G.R., Kern T.S., Matsuyama S, & Subauste C.S. (2016). Ligation of CD40 in Human Müller Cells Induces P2X7 Receptor–Dependent Death of Retinal Endothelial Cells. Investigative Ophthalmology & Visual Science, 57(14), 6278-6286.
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8

Cytokine Assay of Cell Samples

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Supernatants from cell culture, serum and tissue were assayed for mouse IL-1β, TNF-α, IL-6 and human IL-1β (eBioscience) according to manufacturer’s instructions.
Yu X., Zhao Q., Zhang X., Zhang H., Liu Y., Wu X., Li M., Li X., Zhang J., Ruan X, & Zhang H. (2017). Celastrol ameliorates inflammation through inhibition of NLRP3 inflammasome activation. Oncotarget, 8(40), 67300-67314.
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9

Cytokine Profiling of Cell Cultures

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Cytokine concentration in culture supernatant was measured by commercial ELISA kits: human IL-1β (ebioscience), human TNF-α, IL-18, and IFN-γ, IL-17A (R&D systems), and murine IL-1β, TNF-α (R&D systems) according to manufacturer’s instructions. IL-6, MCP-1, IL-8, IL-18 and IFN-γ from human PBMCs were measured by LEGENDplex™ Human Inflammation Panel (Biolegend) according to manufacturer’s instructions.
Ma H., Chan J.F., Tan Y.P., Kui L., Tsang C.C., Pei S.L., Lau Y.L., Woo P.C, & Lee P.P. (2021). NLRP3 Inflammasome Contributes to Host Defense Against Talaromyces marneffei Infection. Frontiers in Immunology, 12, 760095.
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10

Cytokine and Enzyme Activity Assays

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All biochemical analysis methods are described in detail in Supplemental Information. Cytokine secretion was measured by Multiplex (Figure 3A, B IL-1β, TNFα from Life Technologies) or ELISA (human IL-1β from eBioscience 88-7261-22; S100A8 from Thermo EHS100A8) according to manufacturers’ protocols. Elastase (ab118971) and Cathepsin G (ab204693) enzymatic activity were assessed using kits (Abcam) according to manufacturer’s instructions.
Goldberg E.L., Asher J.L., Molony R.D., Shaw A.C., Zeiss C.J., Wang C., Morozova-Roche L.A., Herzog R.I., Iwasaki A, & Dixit V.D. (2017). β-hydroxybutyrate deactivates neutrophil NLRP3 inflammasome to relieve gout flares. Cell reports, 18(9), 2077-2087.
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