Anti glut4

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

Anti-GLUT4 is a laboratory reagent used for the detection and quantification of the GLUT4 protein, which is a glucose transporter found in fat and muscle cells. It can be used in various analytical techniques, such as Western blotting and immunohistochemistry, to study the expression and localization of GLUT4 in biological samples.

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Lab products found in correlation

Anti sx16, by Synaptic Systems (2 mentions) Anti syntaxin 4, by Synaptic Systems (2 mentions) Anti snx27, by Proteintech (1 mentions) Anti vps35, by Proteintech (1 mentions) Anti sx4, by Synaptic Systems (1 mentions) Anti efr3, by Merck Group (1 mentions) Anti ha, by Fortrea (1 mentions) Anti pan akt, by Cell Signaling Technology (1 mentions) Hek293t cell, by American Type Culture Collection (1 mentions) 3t3 l1 fibroblast, by American Type Culture Collection (1 mentions) Anti vamp4, by Synaptic Systems (1 mentions) Anti snap23, by Synaptic Systems (1 mentions) Brain derived neurotrophic factor (bdnf), by RayBiotech (1 mentions) Glp 1, by RayBiotech (1 mentions) Anti β actin, by Thermo Fisher Scientific (1 mentions) Antibodies against akt2, by Cell Signaling Technology (1 mentions) Anti ampkα2, by Santa Cruz Biotechnology (1 mentions)

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GLUT4 (11 citations)

5 protocols using anti glut4

Western Blot Analysis of GLUT4 and Trafficking Proteins

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Samples were thawed and combined with Laemmli sample buffer and heated to 65°C for 10 min prior to SDS‐PAGE and immunoblotting. Blots were visualized using LICOR infra‐red fluorescence detection and quantified using company proprietary software. The antibodies used in this study were anti‐GLUT4 (Thermo Fisher Scientific Cat# PA1‐1065, RRID:AB_2191454), anti‐Sx16 (Synaptic Systems Cat# 110162, RRID:AB_887799), anti‐Sx4 (Synaptic Systems Cat# 110042, RRID:AB_887853), anti‐SNX1 (Proteintech Cat# 10304‐1‐AP, RRID:AB_2192217; the band at 70 kDa present in both 3 T3‐L1 adipocytes and skeletal muscle was quantified), anti‐SNX27 (Proteintech Cat# 16329‐1‐AP, RRID:AB_10888628), anti‐VPS35 (Proteintech Cat# 10236‐1‐AP, RRID:AB_2215216) and anti‐MAPK (Santa Cruz Biotechnology Cat# sc‐514,302, RRID:AB_2571739).

Livingstone R., Bryant N.J., Boyle J.G., Petrie J.R, & Gould G.W. (2022). Diabetes is accompanied by changes in the levels of proteins involved in endosomal GLUT4 trafficking in obese human skeletal muscle. Endocrinology, Diabetes & Metabolism, 5(5), e361.

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Antibody Detection and Immunoblotting Protocols

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Anti-Syntaxin 4 was from Synaptic Systems (Germany; #235003 and 110042, RRID:AB_887853, respectively). Anti-GAPDH [#AM4300. RRID:AB_2536381], anti-GLUT4 [# PA1-1065, RRID:AB_2191454], Anti-EFR3A [#PA5-54694, RRID_AB:2640925] and anti-PI4K [#PA5-28570, RRID:AB-2546046] were from ThermoFisher (Renfrew, U.K.). Anti-EFR3 was from Sigma (Dorset U.K.; #HPA023092) and anti-PI4K-IIIα was from AbCam (Cambridge, U.K.; #111565). Anti-HA was from Covance (Suffolk, U.K.; mouse monoclonal MMS101P, RRID:AB_2314672) or from Roche (Wellwyn Garden City, U.K.; RRID:AB_2687407). The Alexa Fluor 647-conjugated Anti-HA antibody for dSTORM experiments was from Invitrogen (Renfrew, U.K.; mouse monoclonal #26183-A647, RRID:AB_2610626). Secondary antibodies were from LICOR Biosciences (Cambridge U.K.; donkey anti-rabbit: #925 68023, RRID:AB_2814907; goat anti-mouse #925 68070, RRID:AB_2651128; goat anti-mouse #926 32210, RRID:AB_621842 and goat anti-rat [for FACS] #A-21247, RRID:AB_141778).

Koester A.M., Geiser A., Laidlaw K.M., Morris S., Cutiongco M.F., Stirrat L., Gadegaard N., Boles E., Black H.L., Bryant N.J, & Gould G.W. (2022). EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes. Bioscience Reports, 42(7), BSR20221181.

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Adipocyte Differentiation and GLUT4 Trafficking

Cited 3 times

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HEK293T cells (Cat# CRL-3216 RRID:CVCL_0063) and 3T3-L1 fibroblasts (Cat# CCL-92.1, RRID:CVCL_0123) were obtained from the American Tissue Culture Collection and grown and differentiated into adipocytes as outlined in (Sadler et al., 2019 (link)). HeLa cells expressing HA-GLUT4-GFP were as described previously (Morris et al., 2020 (link)). AMPK α1/ α2 knockout mouse embryonic fibroblasts (MEFs) and control MEFs were cultured as described previously (Laderoute et al., 2006 (link)). Antibodies used: anti-GLUT4 (Thermo Fisher Scientific Cat# PA1-1065, RRID:AB_2191454), anti-Sx16 (Synaptic Systems Cat# 110 162, RRID:AB_887799), anti-mVps45 (Novus Cat# NB100-2431, RRID:AB_2272935), anti-VAMP4 (Synaptic Systems Cat# 136 002, RRID:AB_887816), anti-SNAP23 (Synaptic Systems Cat# 111 202, RRID:AB_887788) and anti-Syntaxin 4 (Synaptic Systems Cat# 110 042, RRID:AB_887853). A phospho-specific antibody raised in rabbits against the N-terminal 16 amino acids of murine Sx16 with Thr-7 chemically phosphorylated and recognizing Thr-7 in the Sx16 N-terminus only when phosphorylated, was generated by Antagene Inc (Mountain View, CA, USA) and purified using affinity chromatography. Anti-AKT (pan) (Cell Signaling Technology Cat# 2920, RRID:AB_1147620) and anti-AKT Phospho-S473 (Cell Signaling Technology Cat#4058), RRID:AB_331168) were used as outlined (Bremner et al., 2022 (link)).

Bremner S.K., Berends R., Kaupisch A., Roccisana J., Sutherland C., Bryant N.J, & Gould G.W. (2023). Phosphorylation of the N-terminus of Syntaxin-16 controls interaction with mVps45 and GLUT4 trafficking in adipocytes. PeerJ, 11, e15630.

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Molecular Mechanisms of CDCA-Mediated Metabolic Regulation

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CDCA was purchased from Sigma-Aldrich (Darmstadt, Germany; CAT#: C9377); and both AlCl₃ and sodium bicarbonate (NaHCO₃) were purchased from Oxford Lab Chem (Mumbai, India).
Western blot antibody of pSer473-Akt was obtained from MyBioSource Inc. (San Diego, CA, USA; CAT#: MBS003071), whereas the remaining primary antibodies were obtained from ThermoFisher Scientific (Waltham, MA, USA); viz., anti-pSer307-IRS1 (CAT#: 44-813G), anti-T-Akt (CAT#: MA5-14916), anti-GLUT4 (CAT#: MA1-83191), anti-pSer133-CREB (CAT#: MA5-11192), anti-T-CREB (CAT#: MA1-083), anti-BACE1 (CAT#: MA1-177) and anti-β-Actin (CAT#: PA1-183).
Sources for enzyme-linked immunosorbent assay (ELISA) kits are mentioned in brackets; Aβ₄₂ (MyBioSource Inc.; CAT#: MBS726579), tissue insulin level (MyBioSource Inc.; CAT#: MBS724709), BDNF (RayBiotech, Peachtree Corners, GA, USA; CAT#: ELR-BDNF), GLP-1 (RayBiotech; CAT#: EIAR-GLP1) and PPARγ (CUSABIO, Houston, TX, USA; CAT#: CSB-E08624r).

Bazzari F.H., Abdallah D.M, & El-Abhar H.S. (2019). Chenodeoxycholic Acid Ameliorates AlCl3-Induced Alzheimer’s Disease Neurotoxicity and Cognitive Deterioration via Enhanced Insulin Signaling in Rats. Molecules, 24(10), 1992.

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Antibody Panel for Insulin Signaling

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Antibodies against Akt2 (catalog #3063) and HKII (catalog #2867) were purchased from Cell Signaling Technology. Anti‐ GLUT4 (catalog #PA1‐1065; Thermo Fisher Scientific); Anti‐IR (kindly provided by Professor Ken Siddle, Cambridge University, Cambridge, UK); anti‐TBC1D4 (catalog #189890, Abcam); anti‐AMPKα₂ (catalog #SC‐19131, Santa Cruz Biotechnology); Anti‐GS (kindly provided by Professor Oluf Pedersen, University of Copenhagen, Copenhagen, Denmark); anti‐PDH (custom made, Professor D.G. Hardie, University of Dundee, Dundee, Scotland, UK).

Bruhn L., Kjøbsted R., Quist J.S., Gram A.S., Rosenkilde M., Færch K., Wojtaszewski J.F., Stallknecht B, & Blond M.B. (2021). Effect of exercise training on skeletal muscle protein expression in relation to insulin sensitivity: Per‐protocol analysis of a randomized controlled trial (GO‐ACTIWE). Physiological Reports, 9(10), e14850.

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