Leptin elisa kit

Plasma glucose was measured with the enzymatic glucose analyses technique. HbA1C was measured with a high-performance liquid chromatography (HPLC) technique. Insulin was measured using the Lumipulse G1200 system (Fujirebio Inc., Tokyo, Japan). AST, ALT, Gamma-GT, Alkaline Phosphatase, LDL-cholesterol, HDL-cholesterol and triglycerides were measured using enzymatic assays. Cholesterol was measured using a turbidimetric immunoassay. TSH was measured using the Fluorometric Enzyme Immunoassay (FEIA). Free T4 was measured using the equilibrium dialysis method. Leptin was measured using the leptin ELISA kit (Mediagnost, Reutlingen, Germany).

The blood samples from all the subjects before surgery were collected after a 12-h fast. The serum was separated and immediately frozen at −80 °C. The serum biochemical variables were measured in duplicate. Serum glucose, cholesterol, high density lipoprotein (HDL) cholesterol, triglycerides, and FFAs were measured by standard enzymatic methods (Randox Laboratories Ltd., Antrium, UK). The adiponectin levels were measured by an enzyme immunoassay Adiponectin ELISA kit (DRG Diagnostics, Marburg, Germany). The leptin levels were measured by a Leptin ELISA kit (Mediagnost, Reutlingen, Germany). The insulin was analyzed by an immunoradiometric assay (BioSource International, Camarillo, CA, USA). The homeostasis model assessment of insulin resistance index (HOMA-IR) was calculated with the following equation: HOMA-IR = fasting insulin (μIU/mL) × fasting glucose (mg/dL)/405 [28 (link)]. The morbidly obese subjects were also divided in two groups as follows: those with low HOMA-IR (<4.7) and those with high HOMA-IR (>8).