Survivin sirna sc 29499

The siCtrl (sc-37,007), survivin siRNA (sc-29499), XIAP siRNA (sc-37508), and FBXL7 siRNA (sc-62,306) were obtained from Santa Cruz Biotechnology (Dalla, TX). siRNA transfection was performed according to the manufacturer's protocol. Briefly, cells were grown to 60-80% confluence and cultured with antibiotic‐ and serum‐free medium. Add the siRNA duplexes solution directly to the dilute transfection reagent, followed by mixing and incubating for 20 min at RT. The mixture was added to cells in antibiotic‐ and serum‐free medium. After 5-7 h incubation, cells were incubated with 10% FBS and 1% P/S for 24 h.

NgBR siRNA (forward, GGAAAUACAUAGACCUACA; reverse, UGUAGGUCUAUGUAUUUCC) oligonucleotides with 3′ dTdT overhangs were synthesized by QIAGEN (Valencia, CA, USA). The specificity of NgBR siRNA has been validated in our previous publication [12 (link)]. Control siRNA in experiments refers to a non-silencing (NS) siRNA (NSF, UUCUCCGAACGUGUCACGU; NSR, ACGUGACACGUUCGG AGAA) designed and synthesized by QIAGEN. P53 siRNA (sc44218) and survivin siRNA (sc-29,499) were purchased from Santa Cruz (Dallas, TX, USA). Cells were transfected with siRNA using Lipofectamine RNAiMAX reagent (ThermoFisher Scientific). For transient NgBR overexpression experiments, MCF-7 cells were transfected with pIRES-NgBR-HA plasmid DNA using Lipofectamine 2000 reagent (ThermoFisher Scientific).