Nadph
Manufactured by Sangon
Sourced in China
NADPH is a coenzyme that plays a crucial role in various metabolic processes. It serves as a reducing agent, providing electrons for numerous enzymatic reactions within cells. NADPH is essential for antioxidant defense, lipid biosynthesis, and other important cellular functions.
Automatically generated - may contain errors
Lab products found in correlation
Coomassie brilliant blue r 250, by Sangon (2 mentions)
D sorbitol, by Merck Group (1 mentions)
D mannitol, by Merck Group (1 mentions)
Rna extraction kit, by Vazyme (1 mentions)
D fructose 6 p, by Merck Group (1 mentions)
D fructose, by Merck Group (1 mentions)
Reverse transcription kit, by Vazyme (1 mentions)
Ammonium sulfate, by Sangon (1 mentions)
Catalase, by Sangon (1 mentions)
Kanamycin sulfate, by Sangon (1 mentions)
5 aminolevulinic acid, by Merck Group (1 mentions)
Ampicillin sodium, by Sangon (1 mentions)
Taq dna polymerase, by Takara Bio (1 mentions)
Primestar hs dna polymerase with gc buffer, by Takara Bio (1 mentions)
Glucose 6 phosphate dehydrogenase, by Sangon (1 mentions)
Glucose 6 phosphate (g6p), by Sangon (1 mentions)
T4 dna ligase, by Takara Bio (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Nadph, by Merck Group (1 mentions)
Chloramphenicol, by Sangon (1 mentions)
4 protocols using nadph
1
Analytical Standards for Biochemical Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
d -mannitol, d -sorbitol, d -arabitol, xylitol, ribitol, erythritol, glycerol, d -fructose-6-P, and d -fructose were obtained from Sigma-Aldrich (St. Louis, United States). Coomassie brilliant blue R-250, cofactors NADP, NAD, NADH, NADPH, and antibiotics were purchased from Sangon Biotech (Shanghai, China). New England Biolabs or Takara supplied all restriction enzymes. High-fidelity KOD-Fx, 2 × ready-to-use qTaq mixture for PCR were purchased from Toyobo (Japan). RNA extraction kit, reverse transcription kit, and qPCR kit were obtained from Vazyme Biotech Co., Ltd (Nanjing, China). All other chemicals were of analytical grade.
+ Expand
2
Cholesterol Oxidation Enzymatic Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All solvents and reagents were of the highest purity commercially available, unless noted otherwise. Isopropyl-β-d -thiogalactopyranoside (IPTG), acrylamide, N,N′-methylenebisacrylamide, ammonium persulfate, 30% acrylamide, TEMED, glycine, Tris, DTT, ampicillin sodium, kanamycin sulfate, chloramphenicol, hydroxypropyl NADH, NADPH, glucose-6-phosphate, glucose-6-phosphate dehydrogenase, catalase and ammonium sulfate were all purchased from Sangon Biotech (Shanghai, China). Cholesterol, Cholesterol propionate, NADPH, 5-aminolevulinic acid, spinach ferredoxin (spFDX) and spinach ferredoxin reductase (spFDR) were obtained from Sigma-Aldrich (St. Louis, MO, USA). 4-Cholesten-3-one and methyl-β-cyclodextrin were purchased from J&K Scientific Ltd. (Beijing, China). Taq DNA polymerase, PrimeSTAR HS DNA Polymerase (with GC buffer), T4 DNA ligase, restriction endonucleases Nde I and Xho I, T-A cloning kits and DNA MW markers were purchased from Takara (Dalian, China). Cholesterol sodium sulfate was purchased from Ark Pharm Inc. (Libertyville, IL, USA).
3
Enzymatic Characterization of Glucose and Mannose Phosphates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
α-d -Glucose-1-phosphate disodium salt (G1P), α-d -Mannose-1-phosphate sodium salt (M1P), and α-d -Glucose-1, 6-diphosphate potassium salt were purchased from Biosynth Carbosynth (United Kingdom). NADP and NADPH were purchased from Sangon Biotech (China). Phosphoglucose isomerase, phosphomannose isomerase, and glucose-6-phosphate dehydrogenase were purchased from Sigma-Aldrich (United States).
4
Enzymatic Assays of Carbohydrates
Check if the same lab product or an alternative is used in the 5 most similar protocols
d -erythrose, l -erythrulose, d -erythritol, d -arabitol, l -arabinose, d -xylose, d -ribose, and d -xylulose were obtained from Sigma-Aldrich (St. Louis, United States). Coomassie brilliant blue R-250, cofactor NADP, NAD, NADH, NADPH, and antibiotics were purchased from Sangon Biotech (Shanghai, China). The chromatographic media were procured from GE Healthcare Life Sciences (Sweden). All other chemicals were of analytical grade and used as such without any purification.
+ Expand
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!