DOX-HCl was purchased from Beijing HuaFeng United Technology Co. (Beijing, China); OA and 2,2- diphenyl-1-picrylhydrazyl (DPPH) from Aladdin Industrial Corporation (Shanghai, China); dehydrogenated soya phosphatidyl choline (HSPC) from Shanghai Advanced Vehicle Technology Ltd. (Shanghai, China); cholesterol (CHOL) from Acros Organics (Morris Plains, NJ, USA); DSPE-PEG(2000) from Avanti Polar Lipids, Inc. (Alabaster, AL, USA); Sephadex G-25 from GE Healthcare Bio-Sciences AB (Uppsala, Sweden); Sepharose CL-2B from Beijing Solarbio Life Sciences, (Beijing, China); PBS and HEPES from Biosharp (Anhui, China); CHCl3, ethanol, methanol, (NH4)2SO4 (AR grade), and Tween 80 (CP grade) from Sinopharm Chemical Reagent Co. (Shanghai, China); acetonitrile and methanol (HPLC grade) from Thermo Fisher Scientific (Geel, Belgium); trifluoroacetic acid (TFA) from Merck (Darmstadt, Germany); MTT, DMSO, and DMEM from Sigma-Aldrich (St. Louis, MO, USA); FBS from Zhejiang Tianhang Biological Technology Co., Ltd. (Hangzhou, China); and DAPI from KeyGen Biotech. (Nanjing, China).
Methanol and acetonitrile hplc grade
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom, China
Methanol and acetonitrile are high-performance liquid chromatography (HPLC) grade solvents. They are commonly used as mobile phase components in HPLC analysis.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Thermo Fisher Scientific (9 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (4 mentions)
Ethyl acetate, by Thermo Fisher Scientific (2 mentions)
Magnesium sulfate mgso4, by Thermo Fisher Scientific (2 mentions)
Potato dextrose agar (pda), by Thermo Fisher Scientific (2 mentions)
Potato dextrose broth (pdb), by Thermo Fisher Scientific (2 mentions)
Milli q purification system, by Merck Group (2 mentions)
Phenacetin, by Merck Group (2 mentions)
Chlorzoxazone, by Merck Group (2 mentions)
Potassium phosphate monobasic, by Thermo Fisher Scientific (2 mentions)
Phosphoric acid, by Thermo Fisher Scientific (2 mentions)
Sodium sulfate, by Thermo Fisher Scientific (2 mentions)
Piroxicam, by Thermo Fisher Scientific (2 mentions)
Cholesterol chol, by Thermo Fisher Scientific (1 mentions)
Pbs and hepes, by Biosharp (1 mentions)
Chcl3, by Sinopharm (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Dspe peg2000, by Avanti Polar Lipids (1 mentions)
Methanol, by Sinopharm (1 mentions)
21 protocols using methanol and acetonitrile hplc grade
1
Lipid Nanoparticle Formulation and Characterization
2
Lipid-Based Nanoparticle Delivery of ATRA and Anti-PD-1 Antibody
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Hydrogenated soybean phosphatidylcholine (HSPC), 1, 2-distearoyl-sn-glycero -3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (DSPE-PEG2000) were purchased from Lipoid (Germany). Cholesterol and fluorescein labeled PEG-DSPE were from Avanti Polar Lipids (AL, USA). All-trans retinoic acid (ATRA) was obtained from Shandong Liangfu Pharmaceutical Co., Ltd. Other reagents including ethanol, calcium acetate, sodium chloride, potassium chloride and sucrose were of analytical reagent grades and purchased from Sinopharm (Shanghai, China). Fetal bovine serum and hydroxypropyl-β-cyclodextrin (HPCD) were from Sigma-Aldrich (Steinheim, Germany). Acetonitrile and methanol (HPLC grade) were from Thermo Fisher Scientific (Waltham, USA). Therapeutic mouse anti-PD-1 were purchased from BIO X CELL™(USA), InVivoMAb Antibodies,anti-mouse PD-1(CD279,Clone: 29F.1A12).
3
Determination of Phytochemical Compounds
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Acetic acid (Tianjin Chemical Reagent Company, China); xylene (Shanghai Chemical Reagent Company, China); aspirin (Shenyang Aojina Pharmaceutical Company, China); diphenoxylate (Xinxiang Changle Pharmaceutical Company, China); castor oil (Shanghai Aladdin Industrial Corporation, China); and acetonitrile and methanol (HPLC grade) were purchased from Fisher Scientific (England); quercetin, berberine, atractylodin, matrine and patehouli aleohal were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Purity of all ≥98%, China).
4
Analytical Standards and Sample Preparation Protocol
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Analytical standards (purity >98%) were purchased from Cluzeau Info Labo (CIL, Sainte-Foy-La Grande, France), TechLab (Metz, France), Sigma-Aldrich (Saint-Quentin Fallavier, France), and LGC standard (Molsheim, France).
The acetonitrile and methanol (HPLC grade) used for extraction were purchased from Fisher Chemical (Ilkirch, France). Ultrapure water was produced using a Millipore Direct-Ultrapure Water System. Oasis® HLB (divinylbenzene/N-vinylpyrrolidone copolymer) extraction cartridges (500 mg, 6cc, 60 µm) were purchased from Waters Corporation (Guyancourt, France). Empty polypropylene SPE tubes with polyethylene frits were supplied by Supelco (Saint-Quentin Fallavier, France). The POCIS were purchased from Exposmeter SA (Tavelsjö, Sweden). Their pharmaceutical configuration consists of two 0.1-µm PolyEther Sulfone (PES) membranes containing Oasis® HLB sorbent.
The solvents used for the HPLC-HRMS analysis included acetonitrile and water (UPLC/MS grade) purchased from Biosolve (Dieuze, France), and formic acid (99%, LC/MS grade) purchased from Avantor (Deventer, the Netherlands). The lock mass used as an internal reference in the mass spectrometer's ionization source, Leucine Enkephalin (LeuEnk), was purchased from Waters Corporation and the calibrant for the mass spectrometer, sodium formate, was prepared with sodium hydroxide, purchased from Sigma Aldrich.
The acetonitrile and methanol (HPLC grade) used for extraction were purchased from Fisher Chemical (Ilkirch, France). Ultrapure water was produced using a Millipore Direct-Ultrapure Water System. Oasis® HLB (divinylbenzene/N-vinylpyrrolidone copolymer) extraction cartridges (500 mg, 6cc, 60 µm) were purchased from Waters Corporation (Guyancourt, France). Empty polypropylene SPE tubes with polyethylene frits were supplied by Supelco (Saint-Quentin Fallavier, France). The POCIS were purchased from Exposmeter SA (Tavelsjö, Sweden). Their pharmaceutical configuration consists of two 0.1-µm PolyEther Sulfone (PES) membranes containing Oasis® HLB sorbent.
The solvents used for the HPLC-HRMS analysis included acetonitrile and water (UPLC/MS grade) purchased from Biosolve (Dieuze, France), and formic acid (99%, LC/MS grade) purchased from Avantor (Deventer, the Netherlands). The lock mass used as an internal reference in the mass spectrometer's ionization source, Leucine Enkephalin (LeuEnk), was purchased from Waters Corporation and the calibrant for the mass spectrometer, sodium formate, was prepared with sodium hydroxide, purchased from Sigma Aldrich.
5
In Vitro Metabolism of Pinocembrin
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Pinocembrin
(≥95%), tacrine hydrochloride (≥98%), hydroxytacrine
maleate, i.e., 1-hydroxytacrine (≥98%), and
paracetamol (≥98%) were obtained from Cayman Chemical. Fluvoxamine
maleate (≥97%), phenacetin (≥98%), diazepam (∼98%),
chlorzoxazone (≥98%), nicotinamide adenine dinucleotide phosphate
(NADPH) (≥98%), and Tween 20 were procured from Sigma-Aldrich.
Caffeine was obtained from the Indian Pharmacopoeia Commission. RLM
(lot no. RT059-D) and HLM (pool of 50 donors; lot no. PL050D-A) were
procured from Gibco. MS-grade acetonitrile and formic acid as well
as HPLC-grade methanol and acetonitrile were obtained from Thermo
Fisher Scientific. Magnesium chloride and monobasic potassium phosphate
were purchased from Rankem. PEG-400 and dimethylsulfoxide (DMSO) were
procured from Loba Chemie. Ultrapure water was obtained from a water
purification system (Direct-Q 3, Merck-Millipore). Characterization
data of the test candidate (pinocembrin) are presented inFigures S1–S4 .
(≥95%), tacrine hydrochloride (≥98%), hydroxytacrine
maleate, i.e., 1-hydroxytacrine (≥98%), and
paracetamol (≥98%) were obtained from Cayman Chemical. Fluvoxamine
maleate (≥97%), phenacetin (≥98%), diazepam (∼98%),
chlorzoxazone (≥98%), nicotinamide adenine dinucleotide phosphate
(NADPH) (≥98%), and Tween 20 were procured from Sigma-Aldrich.
Caffeine was obtained from the Indian Pharmacopoeia Commission. RLM
(lot no. RT059-D) and HLM (pool of 50 donors; lot no. PL050D-A) were
procured from Gibco. MS-grade acetonitrile and formic acid as well
as HPLC-grade methanol and acetonitrile were obtained from Thermo
Fisher Scientific. Magnesium chloride and monobasic potassium phosphate
were purchased from Rankem. PEG-400 and dimethylsulfoxide (DMSO) were
procured from Loba Chemie. Ultrapure water was obtained from a water
purification system (Direct-Q 3, Merck-Millipore). Characterization
data of the test candidate (pinocembrin) are presented in
6
Analytical Reagents for HPLC Analysis
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HPLC-grade methanol and acetonitrile and analytical reagent grade ethyl acetate, formic acid (99%), and dimethyl sulfoxide (99.9% DMSO) were obtained from Thermo Fisher Scientific (Loughborough, UK). Magnesium sulfate (MgSO4) was obtained from Thermo Fisher Scientific (Kandel, Germany). Potato dextrose agar (PDA) and Potato dextrose broth (PDB) was obtained from Thermo Fisher Scientific (Basingstoke, UK). Ultrapure water (<18 MΩ/cm) was obtained from a Milli-Q purification system (Millipore Corp., Bedford, MA, USA).
7
Avermectin B2 Emulsifiable Concentrate Formulation
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Technical avermectin B2
(95%) was purchased from Qilu Pharmaceutical Co., Ltd. (China). Commercial
avermectin B2 emulsifiable concentrate (5 wt %) was purchased from
Shouguang, Shandong (China). The polymerized isocyanate (MDI, 30%
NCO content) was obtained from Wanhua Chemical Group Co., Ltd. (China),
while TEOA was purchased from Tianjin Yongda Chemical Reagent Co.,
Ltd. (China). The fatty alcohol polyoxyethylene polyoxypropylene ether
(500#) emulsifier was purchased from Jiangsu Xinde Biological Technology
Co., Ltd. (China), while the dispersant (Agrilan 700) and the defoaming
agent (SAG*1522) were provided by Nanjing Jierun Technology Co., Ltd.
(China). N-Methyl-2-pyrrolidone (NMP, analytical
reagent) and glacial acetic acid (analytical reagent) were purchased
from Sinopharm Chemical Reagent Co. (Beijing, China). HPLC-grade methanol
and acetonitrile were purchased from Thermo Fisher Scientific Inc.
(USA). Purified water (Wahaha, China) was used for all reactions and
treatment processes. All other chemicals were of analytical grade
and were used without further purification.
(95%) was purchased from Qilu Pharmaceutical Co., Ltd. (China). Commercial
avermectin B2 emulsifiable concentrate (5 wt %) was purchased from
Shouguang, Shandong (China). The polymerized isocyanate (MDI, 30%
NCO content) was obtained from Wanhua Chemical Group Co., Ltd. (China),
while TEOA was purchased from Tianjin Yongda Chemical Reagent Co.,
Ltd. (China). The fatty alcohol polyoxyethylene polyoxypropylene ether
(500#) emulsifier was purchased from Jiangsu Xinde Biological Technology
Co., Ltd. (China), while the dispersant (Agrilan 700) and the defoaming
agent (SAG*1522) were provided by Nanjing Jierun Technology Co., Ltd.
(China). N-Methyl-2-pyrrolidone (NMP, analytical
reagent) and glacial acetic acid (analytical reagent) were purchased
from Sinopharm Chemical Reagent Co. (Beijing, China). HPLC-grade methanol
and acetonitrile were purchased from Thermo Fisher Scientific Inc.
(USA). Purified water (Wahaha, China) was used for all reactions and
treatment processes. All other chemicals were of analytical grade
and were used without further purification.
8
Mycotoxin Standard Detection Protocol
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HPLC-grade methanol and acetonitrile, analytical reagent grade methanol, ethyl acetate, formic acid (99%), and dimethyl sulfoxide (99.9% DMSO) were obtained from Thermo Fisher Scientific (Loughborough, UK). Magnesium sulfate (MgSO4) was obtained from Thermo Fisher Scientific (Kandel, Germany). Potato dextrose agar (PDA) and Potato dextrose broth (PDB) were obtained from Thermo Fisher Scientific (Basingstoke, UK). Ultrapure water (<18 MW/cm) was obtained from a Milli-Q purification system (Millipore Corp., Bedford, MA, USA).
Aflatoxins (AFB1 and AFB2), Ochratoxin A (OTA), Fumonisins (FB1 and FB2), (Zearalenone (ZEA) and Deoxynivalenol (DON) standards were obtained from Sigma-Aldrich (St. Louis, MO, USA).
Aflatoxins (AFB1 and AFB2), Ochratoxin A (OTA), Fumonisins (FB1 and FB2), (Zearalenone (ZEA) and Deoxynivalenol (DON) standards were obtained from Sigma-Aldrich (St. Louis, MO, USA).
9
Posaconazole and Itraconazole Quantification
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Posaconazole was purchased from Selleckchem (Houston, TX, USA). Itraconazole was a kind gift from Nifty Labs PVT Ltd., Hyderabad, India. HPLC grade methanol and acetonitrile (Fisher Scientific UK Ltd., Loughborough, Leicestershire, UK), analytical grade potassium dihydrogen orthophosphate (Riedel-de-Haën, Germany), and high purity distilled water were used. Noxafil 40 mg/mL oral suspension (Patheon Inc., Ontario, Canada, BN 3005A) was purchased from Schering-Plough S.A.
10
Phytochemical Analysis of Alisma Medicinal Extracts
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AR (Batch number: DD6081) and AMR (Batch number: DD8061) were purchased from Beijing Huamiao Pharmaceutical Co. Ltd. (Beijing, China) and their quality was in accordance with the Chinese Pharmacopoeia (2015 Version). The alisol B, alisol B-23 acetate, alisol C, alisol C-23 acetate, and atractylenolide I were purchased from Chroma-Biotechnology Co. Ltd. (Chengdu, China). Honeywell Burdick & Jackson Company (NJ, USA) provided HPLC-grade methanol and acetonitrile, and formic acid (MS grade) was from Fisher Scientific (Spain). Deionized water was obtained using the Milli-Q system (Millipore, MA, USA). Cell culture reagents, and the Nile red dye and cell counting kit-8 (CCK-8 kit) were respectively purchased from Thermo Fisher Scientific (MA, USA) and Beijing Solarbio Science & Technology Co. Ltd. DMSO and oleic acid were from Sigma-Aldrich (MO, USA). Other compounds were of analytical grade.
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