β d glucosidase

β-D-glucosidase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., Shanghai, China; methanol (chromatographic pure), ethyl acetate (chromatographic pure), citric acid, disodium hydrogen phosphate, and anhydrous sodium carbonate were obtained from Tianjin Damao Chemical Reagent Factory, Tianjin, China; concentrator plus vacuum concentrator, ultra-low temperature refrigerator, electronic balance, ultrasonic cleaner, pipette gun were obtained from Eppendorf, Hamburg, Germany; thermostatic bath was obtained from PolyScience, Niles, IL, USA; acid meter, Trace1300 gas chromatograph, Trace ISQ mass spectrometer (GC-MS), and NanoDrop 2000 nucleic acid analyzer were obtained from Thermo, Waltham, MA, USA; high-efficiency plant total RNA extraction kit, UnionScript First-strand cDNA synthesis mix for qPCR kit, and real-time fluorescence quantitative SYBR GreenⅠMaster kit were purchased from Beijing Jinsha Biological Co., Ltd., Beijing, China; LightCycler 480 quantitative PCR instrument was obtained from Roche, Basel, Switzerland.

GAS (6), HBA (8), S-(4-hydroxybenzyl)glutathione (11), parishin J (12), parishin B (13), parishin C (15), parishin K (16), parishin A (17), gastrodioside (19) and 4,4’-dihydroxydibenzyl ether (24) were separated and purified from the dried RGE and identified by Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center with purity > 98% by HPLC [31 (link),32 (link)]. β-d-Glucosidase was purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China).
Acetonitrile of chromatographic grade were obtained by the United States Fisher Scientific (Waltham, MA, USA). Formic acid of chromatographic grade was purchased from Tianjin Kermel Chemical Reagent Co., Ltd. (Tianjin, China). Calibration solution of pH 6.86 and 9.18 were purchased from Shanghai Solarbio Life Science Co., Ltd. (Shanghai, China). Hydrochloric acid of analytical grade was purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Experimental water was obtained by Hangzhou Wahaha Group Co., Ltd. (Hangzhou, China).