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Xcalibar 3

Manufactured by Thermo Fisher Scientific

The XCalibar 3.0 is a high-performance analytical instrument designed for laboratory applications. It provides advanced capabilities for precise measurements and data analysis. The core function of the XCalibar 3.0 is to facilitate accurate and reliable data acquisition and processing.

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2 protocols using xcalibar 3

1

Urushiol extraction and MS analysis

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In all samples, urushiol was extracted using a single phase Folch procedure42 and analyzed using a Q-Exactive Hybrid Quadrupole-Orbitrap. Prior to urushiol extraction, crystals of CD1a-urushiol were extensively washed with the crystallization mother liquor and dissolved in Tris Buffer Saline (TBS), pH 8.0. MS coupled to a RSLC nano HPLC (Thermo Scientific, Bremen, Germany). Samples were loaded onto a nanoviper pepmap100 trap column (100μm × 2cm) in 2% acetonitrile / 0.1% ammonium acetate at a flow rate of 15 μL / minute. Analytes were separated at a flow rate of 300 μL / minute on a pepmap100 C18 column (75μm × 15cm, Thermo Scientific) using a linear gradient of acetonitrile (2-80%). Up to 12 MS/MS spectra were acquired per cycle with maximum accumulation time of 50 ms and 100 ms for MS1 and MS2, respectively. A SCIEX QTRAP 5500 mass spectrometer was used for MRM-based detection as previously described43 . The 5500 mass spectrometer was operated with unit quadrupole resolution and three MRM transitions were simultaneously monitored in detecting U15 315.2→149.1, 315.2→135.1, 315.2→122.1. Data analysis was performed using a combination of Analyst v1.5.2 and XCalibar 3.0 (Thermo Fisher Scientific).
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2

Urushiol extraction and MS analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
In all samples, urushiol was extracted using a single phase Folch procedure42 and analyzed using a Q-Exactive Hybrid Quadrupole-Orbitrap. Prior to urushiol extraction, crystals of CD1a-urushiol were extensively washed with the crystallization mother liquor and dissolved in Tris Buffer Saline (TBS), pH 8.0. MS coupled to a RSLC nano HPLC (Thermo Scientific, Bremen, Germany). Samples were loaded onto a nanoviper pepmap100 trap column (100μm × 2cm) in 2% acetonitrile / 0.1% ammonium acetate at a flow rate of 15 μL / minute. Analytes were separated at a flow rate of 300 μL / minute on a pepmap100 C18 column (75μm × 15cm, Thermo Scientific) using a linear gradient of acetonitrile (2-80%). Up to 12 MS/MS spectra were acquired per cycle with maximum accumulation time of 50 ms and 100 ms for MS1 and MS2, respectively. A SCIEX QTRAP 5500 mass spectrometer was used for MRM-based detection as previously described43 . The 5500 mass spectrometer was operated with unit quadrupole resolution and three MRM transitions were simultaneously monitored in detecting U15 315.2→149.1, 315.2→135.1, 315.2→122.1. Data analysis was performed using a combination of Analyst v1.5.2 and XCalibar 3.0 (Thermo Fisher Scientific).
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