Si zdhhc5

Human gastric carcinoma cell line MGC-803 was obtained from Shanghai Obio Technology Co., Ltd. The cells were maintained in Dulbecco's Modified Eagle Media (DMEM, Gibco, Carlsbad, CA, United States) supplemented with 10% fetal bovine serum (FBS, Gibco, Carlsbad, CA, United States). The construction of ZDHHC5 silence vector (si-ZDHHC5) was performed by GenePharma (Shanghai, China). The miR-96-5p inhibitor and inhibitor NC were purchased from Thermo (Waltham, MA, United States). MGC-803 cells were inoculated in six-well plates for 24 h with approximately 5 × 10⁵ cells in each well, and then inhibitor NC, miR-96-5p inhibitor, si-ZDHHC5, or miR-96-5p inhibitor + si-ZDHHC5 was transfected into MGC-803 cells by Lipofectamine 2000 (Invitrogen, Carlsbad, CA, United States) per the manufacturer's instructions. Meanwhile, MGC-803 cells without transfection served as the control group. Cells were harvested after 48 h of transfection to perform follow-up experiments.