Lidocaine hcl

Manufactured by Merck Group

Sourced in United States

Lidocaine HCl is a local anesthetic used to numb or block pain in the body. It is a white crystalline powder that is soluble in water and alcohol. Lidocaine HCl is commonly used in medical and dental procedures to provide local anesthesia.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Triton x 100, by Merck Group (2 mentions) Trypsin edta, by Thermo Fisher Scientific (2 mentions) Adenosine triphosphate (atp), by Merck Group (2 mentions) Lipopolysaccharides (lps), by Merck Group (2 mentions) Sodium nitrite, by Merck Group (2 mentions) Glutamate, by Merck Group (2 mentions) Ham s f 12 nutrient mixture dmem f12, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (2 mentions) Dmem f12 with hepes dmem f12 hepes, by Thermo Fisher Scientific (2 mentions) Clove oil, by Merck Group (1 mentions) N vinylcaprolactam, by Merck Group (1 mentions) N n methylenebis acrylamide nmba, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions) Atropine sulfate, by Merck Group (1 mentions) Epinephrine bitartrate, by Merck Group (1 mentions) Scopolamine hbr, by Merck Group (1 mentions) Ca2 mg2, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Merck Group (1 mentions) Phenol red, by Merck Group (1 mentions)

Spelling variants of this product

Lidocaine (154 citations)

18 protocols using lidocaine hcl

Anesthetic Efficacy of Clove Oil and Lidocaine

Check if the same lab product or an alternative is used in the 5 most similar protocols

The anaesthetic effect of clove oil (82%-87% eugenol:Sigma, MO, USA) was
investigated at four concentrations (15, 30, 45, and 60 ppm), three water
salinities (10, 20, and 30 ppt) and three temperatures (20℃, 24℃,
and 28℃). The anaesthetic effect of the combination of two anaesthetics,
clove oil and lidocaine-HCl (Sigma, MO, USA) was examined at three
concentrations of clove oil (15, 30, and 45 ppm) and three concentrations of
lidocaine-HCl (400, 500, and 600 ppm) at 24℃ and 30 ppt. Fish were
exposed to different combinations of salinity, water temperature and anaesthesia
concentrations. The stock solution clove oil was dissolved in 95% ethanol
(Sigma, MO, USA) at a ratio of 1:10 (Cho
& Heath, 2000). Low doses of ethanol have no anaesthetic
effect on fish (Munday & Wilson,
1997). The anaesthetic solution of lidocaine-HCl was neutralized with
1,000 ppm NaHCO₃ (Park et al.,
1998b), which was prepared as the total concentration to anaesthetic
effect.
Exposure time was measured from the time when samples were transferred from a
stock tank to an anaesthetic-containing aquarium (30 L) to when they reached A5
(Table 1) behavior; recovery time was
measured between the time when the anaesthetized samples were transferred to a
sea water tank containing 30 L with sufficient aeration and the time they
reached R6 (Table 1) behavior.

, & Park I.S. (2019). Anaesthetic Efficacy and Physiological Response of Clove Oil and Lidocaine-HCl on River Puffer, Takifugu obscurus and Tiger Puffer, T. rubripes. Development & Reproduction, 23(1), 21-34.

+ Open protocol

+ Expand

Hydrogel Formation and Lidocaine Release

Check if the same lab product or an alternative is used in the 5 most similar protocols

N-Vinylcaprolactam (VCL, 99%), sodium dodecyl sulfate (SDS), N,N′ methylenebis(acrylamide) (NMBA) and lidocaine HCl were obtained from Sigma-Aldrich, UK. HA and APS were purchased from Acrös Organics, UK. Visking® dialysis tubing with molecular weight cut-off (MWCO): 12–14 kDa and thickness: 2 mm was supplied from Medicell Membranes (London, UK). Ultrapure de-ionized water was used in all experiments.

Ozkahraman B., Emeriewen K., Saleh G.M, & Thanh N.T. (2020). Engineering hydrogel nanoparticles to enhance transdermal local anaesthetic delivery in human eyelid skin. RSC Advances, 10(7), 3926-3930.

+ Open protocol

+ Expand

Topical Analgesic Formulation Optimization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lidocaine HCl was obtained from Sigma Aldrich (Munchen, Germany). Ketorolac tromethamine was kindly donated by Al-Hikma Pharmaceuticals, Jordan. Lidocaine HCl and isopropyl myristate were kindly donated by the Jordanian Pharmaceutical Manufacturing Co., Jordan. Brij 97 (polyoxyethylene-10 EO-oleyl alcohol) was obtained from Uniqema (DE, USA). Hydrochloric acid (HCl) and high-performance liquid chromatography (HPLC) grade water and acetonitrile were obtained from Acros Organics, Belgium. Water for preparation was double distilled and deionized. Jojoba oil was obtained from the seeds of jojoba plants planted at the farms of Jordan University of Science and Technology and filtered through a 0.45 μm Millipore filter.

Assaf S.M., Maaroof K.T., Altaani B.M., Ghareeb M.M, & Abu Alhayyal A.A. (2021). Jojoba oil-based microemulsion for transdermal drug delivery. Research in Pharmaceutical Sciences, 16(4), 326-340.

+ Open protocol

+ Expand

Pharmacological Evaluation of Plant Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following drugs and dosage were used: carbachol HCl (Sigma) 2 mg/kg; atropine sulfate (Sigma) 2.5 mg/kg; scopolamine HBr (Sigma) 2 mg/kg; epinephrine bitartrate (Sigma) 1 mg/kg; amphetamine bitartrate 5 mg/kg; lidocaine HCl (Sigma) 40 mg/kg; acetyl salycilic acid,100 mg/kg; morphine HCL, 10 mg/kg; haloperidol (Syntex latino, SA) 1 and 6 mg/kg. Each drug was dissolved in isotonic saline solution (0.9% NaCl) just before use, and the resulting solution was administered by intraperitoneal (i.p.) injection of 0.2 mL. The hydroalcoholic extract of the bark was administered intraperitoneally at the dose of 0.5 g dried plant/kg weight, and dichloromethane extract at 1.25 g dried plant/kg weight.

de la Cabeza Fernández M., Sánchez M., Caceres A., Iglesias I, & Gómez-Serranillos M.P. (2023). Neuropharmacological Effects in Animal Models and HPLC-Phytochemical Profiling of Byrsonima crassifolia (L.) Kunth Bark Extracts. Molecules, 28(2), 764.

+ Open protocol

+ Expand

Spinal Dorsal Funiculus Lidocaine Injection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animals were anaesthetized with isoflurane and a partial laminectomy of the T9 vertebrae was performed to expose spinal level T10. Animals were injected bilaterally with 1 μl lidocaine-HCl (400 mg ml⁻¹, Sigma-Aldrich, St Louis, MO) in ACSF or ACSF alone split between two sites, 250 μm deep in the dorsal funiculus. The dorsal musculature was sutured with 4-0 silk sutures and the skin was closed with surgical staples. The animals were tested on an accelerating rotarod starting at 10 min after injection.

Hollis ER I.I., Ishiko N., Pessian M., Tolentino K., Lee-Kubli C.A., Calcutt N.A, & Zou Y. (2015). Remodelling of spared proprioceptive circuit involving a small number of neurons supports functional recovery. Nature communications, 6, 6079.

+ Open protocol

+ Expand

Isolation and Characterization of Murine Resident Peritoneal Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse RPM were obtained by lavaging the peritoneal cavity with 10 ml PBS containing 0.5% BSA and 2 mM EDTA. Cells were washed three times in PBS before resuspending in DMEM supplemented with 10% FCS, 2 mM L-glutamine, 0.1 mg/ml streptomycin and 100 U/ml penicillin. Cells were seeded on 8-well Lab-Tek chamber slides at 6 × 10⁴ cells per well in 300 µl of media. Non-adherent cells were rinsed off after letting the macrophages adhere for 2 h and used in RBC binding/phagocytosis assays. For flow cytometry-based assays, the cells were seeded on 4 cm bacterial Petri dishes (BD Falcon) and after 2 h the RPMs were lifted using 4 mg/ml lidocaine-HCl (Sigma-Aldrich) and 5 mM EDTA in PBS (Ca²⁺-, Mg²⁺-; Gibco, Invitrogen) and immunochemically labeled for analysis by flow cytometry.

Klaas M., Dubock S., Ferguson D.J, & Crocker P.R. (2023). Sialoadhesin (CD169/Siglec-1) is an extended molecule that escapes inhibitory cis-interactions and synergizes with other macrophage receptors to promote phagocytosis. Glycoconjugate Journal, 40(2), 213-223.

+ Open protocol

+ Expand

Primary Cell Culture Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hanks Balanced Saline solutions (HBSS), Dulbecco's Modified Eagle Medium—Hams'F12 nutrient mixture (DMEM-F12), DMEM-F12 with HEPES (DMEM-F12/HEPES), 0.25% trypsin-EDTA, fetal bovine serum (FBS), and Penicillin-Streptomycin (P/S) were from Gibco (ThermoFisher Scientific, Burlington, ON). ATP, glutamate, lidocaine HCl, Triton X-100, LPS, and sodium nitrite standard solution were from Sigma (Oakville, ON).

Jesudasan S.J., Gupta S.J., Churchward M.A., Todd K.G, & Winship I.R. (2021). Inflammatory Cytokine Profile and Plasticity of Brain and Spinal Microglia in Response to ATP and Glutamate. Frontiers in Cellular Neuroscience, 15, 634020.

+ Open protocol

+ Expand

Cellular Assay Reagents Catalogue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reagents were obtained from the following suppliers: 9-aminoacridine (9-AAA), ethidium bromide, diacetate fluorescein, Alexa Fluor 488 Phalloidin, gentamicin, calcein, Lucifer yellow, phenol red; toluidine blue, lidocaine HCl, procaine HCl, tetracaine HCl and trypsin-EDTA from Sigma; fetal bovine serum (FBS) from Gibco, Invitrogen; carboxyfluorescein from Fluka-biochemist; culture medium RPMI 1640 with L-glutamine from Lonza; NaCl and sucrose from Merck; and phosphate-buffered saline (PBS) without calcium and magnesium ions and with calcium and magnesium ions from Biomed.

Grys M., Madeja Z, & Korohoda W. (2017). Avoiding the side effects of electric current pulse application to electroporated cells in disposable small volume cuvettes assures good cell survival. Cellular & Molecular Biology Letters, 22, 1.

+ Open protocol

+ Expand

Subcutaneous Fat Biopsy Procedure

Check if the same lab product or an alternative is used in the 5 most similar protocols

Subcutaneous WAT (approximately 3 g) biopsies from each of the fifteen cows were harvested as previously described (26 (link)). Briefly, the biopsy site, a 5 × 5 cm area of skin at the tailhead, was shaved, then sanitized with iodine scrub and 75% alcohol, and finally anesthetized with 2% lidocaine HCl (Sigma-Aldrich Co., St. Louis, MO, USA). A 1.5–2.5-cm scalpel incision through the skin and subcutaneous tissue is made by aseptic techniques. Adipose tissue was clamped with tweezers and snipped with scissors. After the samples were rinsed with saline, a portion was rapidly frozen in liquid nitrogen and stored at −80°C until analysis, while the rest was fixed in 4% formalin. The tissue specimens were then embedded in paraffin blocks using routine procedures, followed by hematoxylin and eosin (HE) staining and histopathological examination for morphological changes under a microscope.

Yu H., Gao X., Loor J.J., Jiang Q., Fang Z., Hao X., Shi Z., Fan M., Chen M., Li X., Liu G., Wang Z., Li X, & Du X. (2022). Activation of Transcription Factor EB Is Associated With Adipose Tissue Lipolysis in Dairy Cows With Subclinical Ketosis. Frontiers in Veterinary Science, 9, 816064.

+ Open protocol

+ Expand

Measuring Milk Yield and Serum Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples were collected and measured for 3 consecutive days; we used the average for each measurement in the study. During the collection of blood samples, daily milk yields were recorded at 0530 and 1500 h for 3 consecutive days. The 20-kg allowance of TMR per cow per feeding time was weighed and the weight of the orts recorded at the next feeding time.
Finally, DMI was calculated for 3 consecutive days. Blood samples were taken before morning feeding (approximately 0730-0830 h) from a coccygeal vein and were immediately centrifuged at 3,500 × g for 15 min at 4°C. Serum was obtained and stored at -80°C until analysis.
Liver biopsies were taken once on the last day of blood sample collection by liver needle puncture (Shanghai Surgical Equipment Factory, Shanghai, China) from the right side of each cow between the 11th and 12th intercostal space. After shaving and disinfecting with iodine scrub and 75% alcohol, an area (5 × 5 cm) was anesthetized subcutaneously with 2% lidocaine HCl (Sigma-Aldrich Co., St. Louis, MO). A 3 mm stab incision in the skin was made with a scalpel blade, and multiple liver biopsies (~200 mg) were taken from the incision site using the puncture needle. Liver biopsies were frozen immediately after collection in liquid nitrogen and stored at -80°C.

Shen T., Xu F., Fang Z., Loor J.J., Ouyang H., Chen M., Jin B., Wang X., Shi Z., Zhu Y., Liang Y., Ju L., Song Y., Wang Z., Li X., Du X, & Liu G. (2021). Hepatic autophagy and mitophagy status in dairy cows with subclinical and clinical ketosis. Journal of dairy science, 104(4).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!