C6000 electrochemiluminescence immunoassay analyzer

Participants' blood samples were taken between 6:00 and 8:00 a.m. after an overnight fast, and sent for testing before 11 a.m. The laboratory center of the hospital was responsible for the measurement of serum levels of FT3, FT4, TSH, anti-thyroglobulin (TgAb), and TPOAb, with Roche C6000 Electrochemiluminescence Immunoassay Analyzer (Roche Diagnostics, Indianapolis, IN, USA). The normal range was 0.27–4.20 mIU/L for TSH, 3.10–6.80 pmol/L for FT3, 10–23 pmol/L for FT4, 0–115 IU/L for TgAb and 0–34 IU/L for TPOAb.

All patients were asked to fast after 8 p.m. the previous night and to complete venous blood collection and blood pressure measurement between 6 and 8 a.m. the following morning. All collected blood samples were taken immediately to the hospital laboratory center and tested by 11 a.m. The biochemical parameters were tested included fasting blood glucose (FBG), total serum cholesterol (TC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C). low-density lipoprotein cholesterol (LDL-C), thyroid stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4), anti-thyroglobulin antibody (TGAb), and thyroid peroxidase antibody (TPOAb). FBG, TC, TG, HDL-C, and LDL-C were measured by ARCHITECT C8000 System (Abbott Laboratories, Irving, TX, USA). Blood pressure was measured by Omron HBP-1300 electronic manometer. FT3, FT4, TSH, TgAb, and TPOAb were detected by Roche C6000 Electrochemiluminescence Immunoassay Analyzer (Roche Diagnostics, Indianapolis, IN, USA). The diagnostic criteria were as follows: (1) abnormal glucose metabolism: FBG > 6.1 mmol/l; (2) abnormal lipid metabolism: TC ≥ 5.2 mmol/l or TG ≥ 1.7 mmol/l or LDL-C ≥ 3.4 mmol/l or HDL-C < 1.0 mmol/l (29 (link)); (3) hypertension: diastolic blood pressure ≥ 90 mmHg or systolic blood pressure ≥ 140 mmHg.

Serum samples were obtained from each participant between 6 and 8 a.m after an overnight fast, and promptly delivered to the hospital’s laboratory center for analysis before 11 a.m on the same day. Lipid profiles including total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were detected by the enzymatic colorimetric assays. The fasting blood glucose (FBG) were measured by a Cobas E610 (Roche, Basel, Switzerland). The levels of free thyroxine (FT4), free triiodothyronine (FT3), thyroid-stimulating hormone (TSH), thyroid peroxidase antibodies (TPOAb), and anti-thyroglobulin (TGAb) were assessed using the Roche C6000 Electrochemiluminescence Immunoassay Analyzer (Roche Diagnostics, Indianapolis, IN, USA). As reported in our previous studies, we classified TSH into three groups: <2.5, 2.5–4.2, and ≥ 4.2 mIU/L [22 ].