Measurements were generated from three randomly acquired TEM images from SN and ST with a magnification of 16,000x and coded for blinded analysis. The perimeter length of each mitochondrion was analyzed by a single trained technician using MeasureIT software (Olympus Soft Imaging Solutions). Mitochondria were identified by presence of identifiable cristae and distinct double membrane; however, mitochondria with unclear morphology were avoided.
Measureit software
Manufactured by Olympus
MeasureIT is a software application developed by Olympus for scientific and medical laboratory use. It provides tools for accurate measurement and analysis of digital images and data. The core function of MeasureIT is to enable users to perform precise quantitative measurements on digital samples and specimens.
Automatically generated - may contain errors
2 protocols using measureit software
1
Mitochondrial Morphology Analysis
2
Embryoid Body Characterization Protocol
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ES cell colony morphology, EBs development, and EBs viability were monitored on Olympus CKX41 inverted microscope under either phase contrast or fluorescent light as appropriate. EBs were collected on 40 µm cell strainers, and washed twice with PBS removing both medium and cell debris. EBs viability was determined after stained for 30 min in dark at ambient temperature in PBS containing 200 nM calcein (Life Technologies, http://www.lifetechnologies.com) detecting live cells and 2.5 µM ethidium bromide (Sigma-Aldrich, http://www.sigmaaldrich.com) detecting dead cells. The microscope stage and camera adjustments were operated via Cell D software (Olympus Soft Imaging Solutions, http://www.olympus-sis.com). The images were acquired with Sony color CCD camera CC-12 (Sony Corporation, http://www.sony.com). At least 10 EBs were captured per experimental point. An average mean diameter of EBs was calculated with measureIT software (Olympus Soft Imaging Solutions, http://www.olympus-sis.com).
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