Lympholyte mammal cell separation reagent

PCH (CAS no. 36670‐1; purity ≥98%) was purchased from Sigma‐Aldrich, Oakville, ON, Canada. Lympholyte®‐Mammal cell separation reagent was purchased from CedarLane, Burlington, NC. Anti‐PE MicroBeads, LS Columns, and a QuadroMACS™ separator were from Miltenyi Biotec, Bergisch Gladbach, Germany. CountBright™ absolute count beads and fetal bovine serum (FBS) were purchased from Invitrogen, Carlsbad, CA. Anticoagulant solution, buffered salt solution, nucleic acid dye solution (contains SYTO® 13), anti‐CD24‐PE, and anti‐CD61‐PE were from Mouse Blood In Vivo MutaFlow® Kits (Litron Laboratories, Rochester, NY). Hank's balanced salt solution (HBSS) was purchased from MediaTech, Herndon, VA. Reagents used for flow cytometric micronucleus scoring (anticoagulant solution, buffered salt solution, stock propidium iodide solution, anti‐CD71‐FITC, and anti‐CD61‐PE solutions, stock RNase solution, and malaria biostandards) were from In Vivo Mouse MicroFlow® Kits (Litron Laboratories).
For the lacZ assay, phenyl‐beta‐D‐galactopyranoside (P‐Gal) was purchased from G‐Biosciences, St Louis, MO; proteinase K was from Invitrogen, Burlington, ON, Canada; packaging extract kits were from Agilent Technologies, Mississauga, ON, Canada; all other chemicals were purchased from Sigma‐Aldrich.

Lympholyte^®-Mammal cell separation reagent was from CedarLane, Burlington, ON, Canada. Anti-PE MicroBeads, LS+ Positive Selection Columns and QuadroMACS™ Separator were from Miltenyi Biotec GmbH, Bergisch Gladbach, Germany. CountBright™ Absolute Counting Beads were from Invitrogen, Life Technologies™, Carlsbad, CA, USA. Heat-inactivated foetal bovine serum (FBS) was from PAA Laboratories, Pasching, Austria. Anticoagulant Solution, Buffered Salt Solution, Nucleic Acid Dye Solution (SYTO^®13), Anti-CD24-PE and Anti-CD61-PE were from the Prototype Mouse MutaFlow^® kit. This kit and the micronucleus analysis kit (In Vivo Mouse MicroFlow^® Basic) were from Litron Laboratories, Rochester, NY, USA. N-Nitroso-N-Ethylurea (ENU, cat. no. 3385) was from Sigma Aldrich Norway AS, Oslo, Norway. Low melting point agarose (NuSieve^®GTG^®Agarose) and Gelbond^® films were from Lonza, Rockland, ME, USA. SYBR^®Gold Nucleic Acid Gel Stain (10,000× concentrate in DMSO) was from Life Technologies™, Carlsbad, CA, USA. DL-Methionine (cat. no. CA.10850), Torula Yeast Lake States (cat. no. 2184-55), Mineral Mix with Se omitted (cat. no. TD.80313) and Vitamin Mix (cat. no. CA.40060) were from Harlan Laboratories, Madison, WI, USA. Corn oil, calcium carbonate (CaCO₃) and sucrose were from Fôrtek, Center for Feed Technology, Aas, Norway.

Lympholyte®-Mammal cell separation reagent was purchased from CedarLane (Burlington, ON, Canada). Anti-PE MicroBeads, LS + Positive Selection Columns and a QuadroMACS™ Separator were from Miltenyi Biotec (GmbH, Bergisch Gladbach, Germany). CountBright™ Absolute Counting Beads were from Invitrogen, Life Technologies™ (Carlsbad, CA, USA). Heat-inactivated fetal bovine serum (FCS) was from PAA Laboratories (Pasching, Austria). DMEM medium, trypsin/EDTA solution, penicillin/streptomycin was from BioWhittaker™ (Walkersville, MD, USA). GlutaMAX™ Supplement, was from Gibco (Grand Island, NY, USA). Anticoagulant solution, buffered salt solution, nucleic acid dye solution (SYTO^®13), anti-CD24-PE and anti-CD61-PE were from the Prototype In Vivo Mouse MutaFlow^® Kit, Litron Laboratories (Rochester, NY, USA). N-ethyl-N-nitrosourea (ENU, CAS 759–73–9, cat. no. N3385), potassium dichromate (cat. no. 207802), Hydrogen peroxide solution 30 wt. % in H₂O (cat. no. 216763) and DMSO were from Sigma Aldrich (St. Louis, MO, USA). Cell proliferation kit (MTT) (cat. no. 11465007001) was from Roche Diagnostic (Roche Inc., Mannheim, Germany).