Biomag goat anti rat igg

Manufactured by Qiagen

Sourced in Netherlands, Germany

The BioMag goat anti-rat IgG is a magnetic bead-based reagent designed for the separation and purification of rat immunoglobulin G (IgG) from complex samples. The beads are coated with goat anti-rat IgG antibodies, which bind to the target rat IgG molecules, allowing for their magnetic separation and extraction. This product can be used in various immunoassay and purification applications that require the isolation of rat IgG.

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Lab products found in correlation

Anti cd3ε 145 2c11, by BioLegend (1 mentions) Facsaria 3, by BD (1 mentions) Streptavidin conjugated microbeads, by Miltenyi Biotec (1 mentions) Facs fortessa, by BD (1 mentions) Facscanto, by BD (1 mentions) Sh800 cell sorter, by Sony (1 mentions) Anti cd31 390, by BioLegend (1 mentions) Myelin removal beads 2, by Miltenyi Biotec (1 mentions) Facsmelody, by BD (1 mentions) Collagenase 4, by Thermo Fisher Scientific (1 mentions) Dnase 1, by Roche (1 mentions) Facsaria, by BD (1 mentions) Anti cd19, by Thermo Fisher Scientific (1 mentions) Anti bp1, by Thermo Fisher Scientific (1 mentions) Anti cd43, by BD (1 mentions) Anti cd2, by BD (1 mentions)

Spelling variants of this product

BioMag (7 citations) BioMag goat anti-rat IgG beads (5 citations) BioMag goat anti-rat IgG magnetic beads (2 citations)

8 protocols using biomag goat anti rat igg

Comprehensive Antibody Panel for Immunological Assays

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The following antibodies were purchased from BioLegend (San Diego, CA, USA): anti-CD3ε (145-2C11; Cat. No. 100331), anti-CD28 (37.51; Cat. No. 102112), anti-IFN-γ (XMG1.2; Cat. No. 505827), anti-IL-4 (11B11; Cat. No. 504115), anti-CD8α (53-6.7; Cat. No. 100735), anti-I-A/I-E (M5/114.15.2; Cat. No. 107610), anti-NK-1.1 (PK136; Cat. No. 108712), anti-CD25 (PC61; Cat. No. 102031), anti-TCRγδ (UC7-13D5; Cat. No. 107510) and anti-CD62L (MEL-14; Cat. No. 104404). The following secondary antibodies were purchased from Qiagen N.V. (Venio, Netherlands): BioMag goat anti-rat IgG (Cat. No. 310107) and goat anti-mouse IgG (Cat. No. 310007). The anti-Batf3 antibody (sc-162246) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Lee W., Kim H.S., Hwang S.S, & Lee G.R. (2017). The transcription factor Batf3 inhibits the differentiation of regulatory T cells in the periphery. Experimental & Molecular Medicine, 49(11), e393-.

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Isolation and Culture of Invariant NKT Cells

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Thymocytes from Rbpj^+/+ or Rbpj^−/− mice were purified by incubating in anti-CD4 and anti-CD8α Ab followed by BioMag goat anti-Rat IgG (QIAGEN, Hilden, Germany). The resulting double-negative thymocytes were then incubated with CD8α, CD5, TCRγδ, B220, NK1.1, TCRβ, and CD4 and sorted for CD4⁻CD8α⁻TCRγδ⁻B220⁻NK1.1⁻TCRβ⁺CD5^high thymocytes using a FACSAriaIII (BD Biosciences). The sorted cells were cultured in the presence of 50 ng/ml recombinant mouse IL-15 (Miltenyi Biotec) for 8 d.

Ishifune C., Tsukumo S.I., Maekawa Y., Hozumi K., Chung D.H., Motozono C., Yamasaki S., Nakano H, & Yasutomo K. (2019). Regulation of membrane phospholipid asymmetry by Notch-mediated flippase expression controls the number of intraepithelial TCRαβ+CD8αα+ T cells. PLoS Biology, 17(5), e3000262.

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Isolation and Flow Cytometric Analysis of Murine Thymocytes

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Thymocytes were isolated by mechanical disruption. For analyzing DN compartment, total thymocytes were incubated with antibodies against lineage markers such as Ter119, CD11b, Ly6G/Gr1, IgM, CD19, CD4, CD8α, TCRβ, TCRγδ, NK1.1 and CD49b and lineage positive cells were removed by BioMag goat anti-rat IgG (QIAGEN) or streptavidin conjugated microbeads (Miltenyi Biotec). Total thymocytes or cells after depletion were labeled with fluorochrome-conjugated antibodies against T-, B-, NK-, ILC- cell markers for phenotypic analysis. Flow cytometric analysis was performed using a two-laser FACSCanto^™ (BD Biosciences) or a three-laser FACS Aria (BD Biosciences). Cell sorting was performed using a three-laser FACS Fortessa (BD Biosciences) or a three-laser SONY SH800 Cell sorter (SONY Biotechnology). The resulting files were uploaded to FlowJo v10 (BD Biosciences) for further analysis.

Hassan A.A., Khan I.U., Abdulmawjood A, & Lämmler C. (2001). Evaluation of PCR Methods for Rapid Identification and Differentiation of Streptococcus uberis and Streptococcus parauberis. Journal of Clinical Microbiology, 39(4), 1618-1621.

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Isolation and Characterization of Hematopoietic and Lung Cell Populations

Cited 3 times

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HSCs and LMPPs were isolated as previously described (44 (link)). Briefly, lineage depletion was carried out by removal with magnetic beads conjugated to BioMag goat anti-rat IgG (Qiagen 310107) of the following marker antibodies: Ter119, B220, CD19, Mac-1, Gr-1, IgM, CD3, CD8a, and DX5. Lineage-negative cells were stained with the following antibodies: HSC (Lin⁻, cKit⁺, Sca-1^int/+, CD34⁺, FLT3⁻) and LMPP (Lin⁻, cKit⁺, Sca-1^int/+, CD34⁺, FLT3⁺).
ALPs (or CLPs) and BLPs were isolated as previously described (46 (link)–48 (link, link)). Briefly, after lineage depletion (Ter119, Mac-1, B220, Gr-1, CD3e; biotin anti-mouse lineage panel Biolegend 133307), bone marrow cells were stained as followed: ALP (Lin⁻, cKit^mid/lo, IL7Ra⁺, Sca-1^lo, FLT3⁺, Ly6D⁻) and BLP (Lin⁻, cKit^mid/lo, IL7Ra⁺, Sca-1^lo, FLT3^+/int, Ly6D⁺).
Pro-B, large pre-B, and small pre-B cells were isolated as previously described (49 (link), 50 (link)). Bone marrow cells were stained as followed: pro-B (CD19⁺, IgM⁻, cKit^lo, CD43⁺), large pre-B (CD19⁺, IgM⁻, cKit⁻, CD43^+/−, CD2⁻), and small pre-B (CD19⁺, IgM⁻, cKit⁻, CD43⁻, CD2⁺).
Lung epithelium cells were stained as followed: CD326 (EpCam)⁺, CD19⁻, CD3⁻, and CD45⁻.
Postsort analysis confirmed purity of cell fractions.

Lion M., Muhire B., Namiki Y., Tolstorukov M.Y, & Oettinger M.A. (2020). Alterations in chromatin at antigen receptor loci define lineage progression during B lymphopoiesis. Proceedings of the National Academy of Sciences of the United States of America, 117(10), 5453-5462.

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Circadian Rhythms in Murine BBB Isolation

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Brains were harvested from mice at time points around the circadian day (ZT2, 6, 10, 14, 18, 22). Brains were finely minced in RPMI media containing 10% CCS, incubated at 37 °C with 2 mg/mL collagenase IV (Gibco) and 200 μg/mL DNase I (Roche) for 30 min with shaking at 250 rpm, pipetting up and down once during incubation. Brain homogenate was demyelinated using myelin removal beads II (Miltenyi Biotec) as instructed by the manufacturer’s protocol. Cell suspensions were filtered using a 100 μm cell strainer. Cells were labeled with anti-CD90 (clone M5/49.4.1, BioXell) for 15 min on ice and incubated with BioMag Goat Anti-Rat IgG (Qiagen) for 30 min with rotation, and magnetically separated (Easy Sep). Supernatant was removed and spun down at 600 × g. Cells were resuspended in 250 μl, labeled with fluorescence-conjugated anti-CD31 (390, BioLegend), anti-CD90 (53-2.1, BioLegend) antibodies sorted using either a BD FACSAria or BD FACSMelody (BD Biosciences). Dead cells were excluded through 4′,6-diamidino-2-phenylindole uptake. Doublets were excluded through FSC-H by FSC-W and SSC-H by SSC-W parameters. Between 10,000 and 20,000 cells were sorted from a single mouse at >95% purity verified by post-sort analysis. Processing of tissue and BBB isolation takes ~4 h. Data from sort were analyzed using FlowJo V10.6 software (TreeStar).

Zhang S.L., Lahens N.F., Yue Z., Arnold D.M., Pakstis P.P., Schwarz J.E, & Sehgal A. (2021). A circadian clock regulates efflux by the blood-brain barrier in mice and human cells. Nature Communications, 12, 617.

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Isolation and Analysis of T Cell Subsets

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CD4⁺Foxp3-GFP⁻ T_eff cells, CD4⁺CD62L⁺Foxp3-GFP⁻ naïve T cells and CD4⁺Foxp3-GFP⁺ T_reg cells were isolated from sDLNs (axillary, brachial, and inguinal) or spleen after depletion of CD8⁺ T cells (anti-CD8α), B cells (anti-IgM), myeloid cells (anti-CD11b) and erythroid cells (anti-Ter119) using magnetic beads conjugated to BioMag goat anti-rat IgG (QIAGEN). Cells remaining after depletion were labeled with fluorochrome-conjugated anti-CD4, CD62L and CD25 for phenotypic analysis. CD4⁺Foxp3-GFP⁻, CD4⁺CD62L⁺Foxp3-GFP⁻ or CD4⁺Foxp3-GFP⁺ cells were sorted using a MoFlo sorter (Cytomation). Sorted cells were subjected to in vitro culture or RNA-seq studies. RNA-seq experiments were performed with two independent sorts of T_reg cells each from sDLNs of wild-type N=7 and Mi2Δ N=2 mice and one sort of T_reg cells from sDLNs of TMKO N=6 mice.

Kashiwagi M., Hosoi J., Lai J.F., Brissette J., Ziegler S.F., Morgan B.A, & Georgopoulos K. (2017). Direct control of regulatory T cells by keratinocytes. Nature immunology, 18(3), 334-343.

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Isolation and Analysis of T Cell Subsets

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Kashiwagi M., Hosoi J., Lai J.F., Brissette J., Ziegler S.F., Morgan B.A, & Georgopoulos K. (2017). Direct control of regulatory T cells by keratinocytes. Nature immunology, 18(3), 334-343.

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Isolation of Large Pre-B Cells

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For isolation of large pre-B cells, also described as early pre-B cells that express pre-BCR and are highly proliferative (for review, see Hardy and Hayakawa 2001 (link)), BM cell preparations were depleted of cells binding to anti-Ter119, anti-Mac-1, anti-Gr-1, anti-IgM, anti-CD3, anti-CD8α, anti-TCRβ, and anti-DX5 (Supplemental Table S1) by removal with magnetic beads conjugated to BioMag goat anti-rat IgG (Qiagen, 310107). The cells remaining after depletion were labeled with fluorochrome-conjugated monoclonal antibodies to B-cell markers (anti-CD19 [eBiosciences, 25-0193], anti-CD43 [BD, 553270], anti-BP1 [Ebiosciences, 11-5995], anti-CD25 [BD, 553075], and anti-CD2 [BD, 553112]) and were used for flow cytometry. Large pre-B cells from wild-type, IkE5^fl/fl CD2-Cre, or IkE5^fl/flERT2-Cre were sorted as CD19⁺CD43⁺BP1⁺ using a MoFlo-Legacy (Cytomation) cell sorter.

Hu Y., Zhang Z., Kashiwagi M., Yoshida T., Joshi I., Jena N., Somasundaram R., Emmanuel A.O., Sigvardsson M., Fitamant J., El-Bardeesy N., Gounari F., Van Etten R.A, & Georgopoulos K. (2016). Superenhancer reprogramming drives a B-cell–epithelial transition and high-risk leukemia. Genes & Development, 30(17), 1971-1990.

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