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Cobas mira plus chemistry analyzer

Manufactured by Roche
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The COBAS MIRA Plus Chemistry Analyzer is a fully automated clinical chemistry analyzer designed for the analysis of a wide range of clinical chemistry tests. It features high-performance sample processing capabilities and advanced analytical technologies to deliver accurate and reliable results.

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Lab products found in correlation

Ifgf23, by Quidel (2 mentions) Mouse epo quantikine elisa, by R&D Systems (2 mentions) Vacutainer tube, by BD (1 mentions) Icp qms, by Agilent Technologies (1 mentions) Mascot hemavet 950fs, by Drew Scientific (1 mentions)

Close spelling variants of this product

Cobas Mira Plus (115 citations) Cobas Mira (115 citations) Cobas analyzer (113 citations) Cobas Mira analyzer (20 citations) Cobas Mira Plus analyzer (14 citations) Cobas-Mira Chemistry Analyzer (6 citations) Mira Plus (6 citations) Roche Chemistry Analyzers (2 citations)

23 protocols using cobas mira plus chemistry analyzer

1

Blood Sample Collection and Analysis

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Peripheral blood samples were collected into tubes containing 5% ethylenediaminetetraacetic acid (EDTA) as anticoagulant for DNA extraction and tubes without anticoagulant for measurement of GAL-3 serum levels. The biological samples were collected from October 2013 to October 2014. Hematological analysis was performed using an electronic cell counter (STKS, Coulter Corporation, FL, USA), and biochemical analysis was performed using a Roche Cobas Mira Plus Chemistry Analyzer (Roche Diagnostics Corporation, Indianapolis, IN, USA).
de Mendonça Belmont T.F., do Ó K.P., Soares da Silva A., de Melo Vilar K., Silva Medeiros F., Silva Vasconcelos L.R., Mendonça dos Anjos A.C., Domingues Hatzlhofer B.L., Pitta M.G., Bezerra M.A., Araújo A.D., de Melo Rego M.J., Moura P, & Cavalcanti M.D. (2016). Single Nucleotide Polymorphisms at +191 and +292 of Galectin-3 Gene (LGALS3) Related to Lower GAL-3 Serum Levels Are Associated with Frequent Respiratory Tract Infection and Vaso-Occlusive Crisis in Children with Sickle Cell Anemia. PLoS ONE, 11(9), e0162297.
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2

Serum Enzyme Analysis for Liver Function

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To evaluate the serum enzymes reflecting liver function, blood samples were centrifuged at 3000 rpm for 10 min and analyzed in a Roche Cobas Mira Plus Chemistry Analyzer (Roche Diagnostics®, IN, USA) as before (27 (link)). Assay Kits for measurement of the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) (Bioclin®, Belo Horizonte, MG, Brazil) were used according to the manufacturer's instructions. A total of 24 samples were evaluated from mice (12 from infected animals and 12 from uninfected of the same age). Results were expressed as units/liter (U/L).
Dias F.F., Amaral K.B., Malta K.K., Silva T.P., Rodrigues G.S., Rosa F.M., Rodrigues G.O., Costa V.V., Chiarini-Garcia H., Weller P.F, & Melo R.C. (2018). Identification of Piecemeal Degranulation and Vesicular Transport of MBP-1 in Liver-Infiltrating Mouse Eosinophils During Acute Experimental Schistosoma mansoni Infection. Frontiers in Immunology, 9, 3019.
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3

Blood Collection and Plasma Extraction

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Following euthanasia by CO2 affixation, blood was collected via vena cava, added with citrate anticoagulant, and centrifuged to separate plasma. Plasma was aliquoted and stored at −80 °C until analysis. Plasma samples were analyzed using COBAS MIRA‐plus chemistry analyzer (Roche) as previously described [32 (link)].
Hong K.U., Doll M.A., Lykoudi A., Salazar-González R.A., Habil M.R., Walls K.M., Bakr A.F., Ghare S.S., Barve S.S., Arteel G.E, & Hein D.W. (2020). Acetylator Genotype-Dependent Dyslipidemia in Rats Congenic for N-Acetyltransferase 2. Toxicology Reports, 7, 1319-1330.
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4

Quantification of Liver and Kidney Biomarkers

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Concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (AF), urea, and creatinine serum levels were quantified with Bioclin commercial kits (Quibasa Química Básica Ltda., Belo Horizonte, MG, Brazil). Results were measured by Cobas Mira plus Chemistry Analyzer (Roche Diagnostics, Basel, Switzerland).
Fraga-Silva T.F., Mimura L.A., Leite L.D., Borim P.A., Ishikawa L.L., Venturini J., de Arruda M.S, & Sartori A. (2019). Gliotoxin Aggravates Experimental Autoimmune Encephalomyelitis by Triggering Neuroinflammation. Toxins, 11(8), 443.
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5

Quantification of Human IgG in Murine Serum

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Alanine amino transferase (ALT) analysis was performed on a Cobas Mira Plus Chemistry Analyzer (Roche Diagnostics, Basel, Switzerland). Samples were diluted as needed to provide adequate volume and bring the results within the linear range of the machine. For qPCR, blood was collected in EDTA microfuge tubes (BD Biosciences; San Diego, CA) and run directly in the PCR mix using Omniklentaq buffer and enzyme (DNA Polymerase Technology, St. Louis, MO) as described (Parker et al., 2012 (link)).
The levels of rVIG were measured in murine serum samples using a quantitative human IgG ELISA. Briefly, goat anti-human (Fc) IgG (Serotec, Oxford, UK) was diluted in a 50 mM sodium-carbonate buffer (pH 9.6) at a 1:500 dilution, and used to coat 96-wells microtiter ELISA plates at 4 °C overnight. Plates were blocked with blocking buffer (PBS + 0.05% Tween 20 + 2% skim milk) at room temperature for 60 min, and serial dilutions of mouse sera were added to wells. Following incubation at room temperature for 1 h on shaker, wells were washed with PBS +0.05% Tween-20. Bound antibody was detected using HRP-conjugated goat anti-human IgG (Serotec, Oxford, UK) at 1:25000 dilution. After 1 h of incubation at room temperature, TMB-Plus substrate was added followed by addition of 1M Sulfuric acid. Optical density was measured at 450 nm.
Parker S., D'Angelo J., Buller R.M., Smee D.F., Lantto J., Nielsen H., Jensen A., Prichard M, & George S.L. (2021). A human recombinant analogue to plasma-derived vaccinia immunoglobulin prophylactically and therapeutically protects against lethal orthopoxvirus challenge. Antiviral Research, 195, 105179.
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6

Plasma Tocopherol and Cholesterol Measurement

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At randomization, participants provided a blood sample (EDTA), collected at least 3 hours after a meal, processed for plasma, buffy coat, and red blood cells, and stored at −70°C (available for 84% of the SELECT participants) (17 (link)). All cases and a representative fraction of the cohort were assayed annually to minimize batch and storage time effects. Plasma α-tocopherol and γ-tocopherol concentrations were determined by reverse-phase high-performance liquid chromatography as described (20 (link)). Cases and sub-cohort participant plasma samples randomly were ordered within individual batches along with 381 blinded pooled quality control (QC) duplicate and triplicate samples. The weighted mean coefficients of variation (CV) were 3.1% and 4.5% for α-tocopherol and γ-tocopherol, respectively. Tocopherol concentrations did not differ based on year of plasma collection, with median α-tocopherol values of 12.9, 12.7, 12.5, 12.3, and 12.6 mg/L for 2005, 2006, 2007, 2008, and 2009, respectively, and 1.7, 1.7, 1.8, 1.7, and 1.9 mg/L, respectively, for γ-tocopherol. Total cholesterol was measured using a Roche Cobas Mira Plus Chemistry Analyzer and Roche Multi-Analyte Serum Calibrator. Samples were run in duplicate and every 10 study samples were bracketed by QC duplicate samples (BioRad Liquid Assayed Multiqual 1, 2, 3) with a CV of 2.5%.
Albanes D., Till C., Klein E.A., Goodman P.J., Mondul A.M., Weinstein S.J., Taylor P.R., Parnes H.L., Gaziano J.M., Song X., Fleshner N.E., Brown P.H., Meyskens FL J.r, & Thompson I.M. (2014). Plasma Tocopherols and Risk of Prostate Cancer in the Selenium and Vitamin E Cancer Prevention Trial (SELECT). Cancer prevention research (Philadelphia, Pa.), 7(9), 886-895.
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7

Plasma Cholesterol Analysis Protocol

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Total plasma cholesterol was measured on a Roche Cobas Mira Plus Chemistry Analyzer, using reagents from Sekisui Diagnostics (Lexington, MA) and following the manufacturer's instructions.
Song X., Diep P., Schenk J.M., Casper C., Orem J., Makhoul Z., Lampe J.W, & Neuhouser M.L. (2016). Changes in relative and absolute concentrations of plasma phospholipid fatty acids observed in a randomized trial of Omega-3 fatty acids supplementation in Uganda. Prostaglandins, leukotrienes, and essential fatty acids, 114, 11-16.
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8

Serum Biomarker Analysis in Mice

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Blood samples were collected from mice at the time of death by cardiac puncture, or by tail bleed according to approved protocols. Routine serum biochemistries including calcium (Ca), phosphate (Pi), alkaline phosphatase (AP), creatinine (Cr), and total serum iron were measured using an automated COBAS MIRA Plus Chemistry Analyzer (Roche Diagnostics, Indianapolis, IN, USA). Serum 1,25D was measured using an enzyme immunoassay (EIA) (Immunodiagnostic Systems Inc., Scottsdale, AZ, USA) according to the manufacturer’s instructions. Serum intact FGF23 concentrations were assessed using a commercial ELISA (Kainos Laboratories International, Tokyo, Japan). FGF23 was also measured using a rodent-specific C-terminal FGF23 ELISA that recognizes full-length FGF23 and peptides 3′ (C-terminal) to the SPC site, according to the manufacturer’s specifications (Immutopics International, San Clemente, CA, USA).
Clinkenbeard E.L., Farrow E.G., Summers L.J., Cass T.A., Roberts J.L., Bayt C.A., Lahm T., Albrecht M., Allen M.R., Peacock M, & White K.E. (2014). Neonatal Iron Deficiency Causes Abnormal Phosphate Metabolism by Elevating FGF23 in Normal and ADHR Mice. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 29(2), 361-369.
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9

Plasma BHB Measurement Protocol

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Blood samples were collected from the coccygeal vein or artery using heparinized Vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) on at 6, 10, and 14 h after the morning feeding d 3 of wk 2 of the covariate period and wk 6 and 8 of the experimental period. Blood samples were immediately centrifuged at 1,500 × g for 15 min at 4°C. The plasma was recovered and frozen (-20°C) until analysis. Plasma samples were composited on an equal-volume basis per cow and sampling week and analyzed for BHB on the Cobas Mira Plus Chemistry Analyzer (F. Hoffmann-La Roche Ltd., Basel, Switzerland) using a commercial kit (Ranbut,
Sadri H., Giallongo F., Hristov A.N., Werner J., Lang C.H., Parys C., Saremi B, & Sauerwein H. (2016). Effects of slow-release urea and rumen-protected methionine and histidine on mammalian target of rapamycin (mTOR) signaling and ubiquitin proteasome-related gene expression in skeletal muscle of dairy cows. Journal of dairy science, 99(8).
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10

Serum Lipid and Liver Enzyme Profiles in N. squamipes

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To evaluate the serum lipid profile and enzymes reflecting liver function, blood samples were collected from N. squamipes by cardiac puncture without anti-coagulant, centrifuged at 3000 rpm for 10 min to obtain sera and analyzed in a Roche Cobas Mira Plus Chemistry Analyzer (Roche Diagnostics®, IN, USA). For biochemical evaluations, assay Kits (Bioclin, Quibasa, Brazil) were used according to the manufacturer's instructions. Animals were not subjected to prior fasting. A total of 26 samples were evaluated from N. squamipes (13 from uninfected and 13 from infected animals). These samples included 6 samples from the same animals used for histological and ultrastructural analyses and other 20 samples obtained from previous expeditions.
Amaral K.B., Silva T.P., Malta K.K., Carmo L.A., Dias F.F., Almeida M.R., Andrade G.F., Martins J.S., Pinho R.R., Costa-Neto S.F., Gentile R, & Melo R.C. (2016). Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment. PLoS ONE, 11(11), e0166979.
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