Immunofluoresence images were captured with a Zeiss Axio Imager Z1 microscope and Zeiss MRm camera, processed and false-colored using Zeiss Axiovision software. High magnification images were captured using Zeiss Apotome structured illumination. Histology (hematoxylin and eosin) images were captured with a Leica DMRB microscope using a Zeiss MRc camera and processed using Zeiss MRGrab software.
Dmrb microscope
Manufactured by Zeiss
The DMRB microscope is a high-performance research microscope designed by Zeiss for a variety of applications. It features advanced optics and a modular design to accommodate various accessories and configurations. The DMRB microscope is capable of providing detailed and accurate observations, but a more detailed description would require interpreting its intended use, which is not within the scope of this request.
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3 protocols using dmrb microscope
1
Multimodal Imaging of Biological Samples
2
CCCP-Induced Mitochondrial Dynamics in SH-SY5Y Cells
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SH-SY5Y cells were plated on glass coverslips and reversely transfected with siRNA and 24 h later with the indicated DNA plasmid. Human GDNF and GFRalpha were added to the cells 24 h after siRNA transfection and 3 h before CCCP treatment. The next day, cells were treated with 10 μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP, Sigma) for 2 or 24 h. Recruitment of parkin to mitochondria (after 2 h CCCP) and removal of mitochondria (after 24 h CCCP) was detected by indirect immunofluorescence using a monoclonal anti-Parkin antibody (PRK8, Santa Cruz Biotechnology) and a polyclonal antibody against HSP60 (Santa Cruz Biotechnology). Nuclei were stained by DAPI. Cells were analysed by fluorescence microscopy using a Leica DMRB microscope and confocal images were taken using a Zeiss LSM710 confocal microscope equipped with a 63× oil objective (NA 1.4). Quantifications are based on three independent experiments. At least 1,500 cells were analysed for each condition.
3
Microscopy Techniques for Cellular Imaging
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IF images were captured with a Zeiss Axio Imager Z1 microscope and Zeiss MRm camera, processed and false-colored using Zeiss Axiovision software. High magnification images were captured using Zeiss Apotome structured illumination. Histology (hematoxylin and eosin) images were captured with a Leica DMRB microscope using a Zeiss MRc camera and processed using Zeiss MRGrab software.
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