Ethanol

Manufactured by Biosolve

Sourced in France

Ethanol is a colorless, volatile, flammable liquid. It is used as a solvent in various laboratory applications.

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Lab products found in correlation

Methanol, by Biosolve (6 mentions) Chloroform, by Biosolve (2 mentions) Potassium sulfate, by Merck Group (2 mentions) Xylene, by Biosolve (2 mentions) Hydrochloric acid (hcl), by Merck Group (2 mentions) Entellan, by Merck Group (2 mentions) Acetonitrile, by Biosolve (2 mentions) Formic acid, by Merck Group (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Ammonium sulfate, by Merck Group (1 mentions) 2 5 dihydroxyacetophenone 2 5 dha, by Merck Group (1 mentions) Citraconic anhydride, by Merck Group (1 mentions) α cyano 4 hydroxycinnamic acid, by Merck Group (1 mentions) Trypsin, by Promega (1 mentions) Hematoxylin, by Merck Group (1 mentions) Butylated hydroxytoluene bht, by Merck Group (1 mentions) Nh4cooh, by Merck Group (1 mentions) Tuning mix, by Agilent Technologies (1 mentions) Ulc ms grade acetonitrile, by Biosolve (1 mentions)

Close spelling variants of this product

Ethanol (LC-MS grade) Absolute ethanol Ethanol absolute (EtOH)

14 protocols using ethanol

HPLC-MS Analytical Reagents

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Water (HPLC MS grade), ethanol, methanol,
and chloroform were obtained from Biosolve BV (Valkenswaard, Netherlands).
Ammonium sulfate and 2,5-dihydroxyacetophenone (2,5-DHA) were acquired
from Sigma-Aldrich (St. Louis, MI, USA).

Grgic A., Nagornov K.O., Kozhinov A.N., Michael J.A., Anthony I.G., Tsybin Y.O., Heeren R.M, & Ellis S.R. (2023). Ultrahigh-Mass Resolution Mass Spectrometry Imaging with an Orbitrap Externally Coupled to a High-Performance Data Acquisition System. Analytical Chemistry, 96(2), 794-801.

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Mass Spectrometry Sample Preparation Protocol

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Water (HPLC and ULC/MS grade), ethanol, acetonitrile
(ACN), trifluoroacetic acid (TFA), and xylene were obtained from Biosolve
B.V. (Valkenswaard, The Netherlands). α-Cyano-4-hydroxycinnamic
acid (CHCA), citraconic anhydride, potassium sulfate, 2,5-dihydroxybenzoic
acid (DHB) 98%, and formic acid (FA) >95% were obtained from Sigma-Aldrich
(St. Louis, MIA). Hydrochloric acid fuming 37% was obtained from Supelco
(Bellefonte, PA). Trypsin was obtained from Promega (Sequencing Grade
Modified Trypsin, Madison, WI). Hematoxylin and Entellan were obtained
from Merck (Darmstadt, Germany). Eosin Y was obtained from J.T. Baker
(Center Valley, PA).

Claes B.S., Krestensen K.K., Yagnik G., Grgic A., Kuik C., Lim M.J., Rothschild K.J., Vandenbosch M, & Heeren R.M. (2023). MALDI-IHC-Guided In-Depth Spatial Proteomics: Targeted and Untargeted MSI Combined. Analytical Chemistry, 95(4), 2329-2338.

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Lipidomic Analysis of Biological Samples

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ULC-MS grade acetonitrile, ethanol, 2-propanol, methanol and ultra-pure water were purchased from Biosolve (Chimie SARL, Dieuze, France; BV, Valkenswaard, Netherlands). Chloroform was purchased from Carlo Erba (Milan, Italy). NH₄COOH, NH₄F, and butylated hydroxytoluene (BHT) were purchased from Sigma Aldrich (Milan, Italy). The Q-TOF calibration solution was prepared in acetonitrile from Agilent Technologies Tuning mix (HP0321 solution, Agilent Technologies, Santa Clara, USA).
N-palmitoyl-d31-D-erythro-sphingosine (d31-Cer[NS]34:1, MW 569.1), N-[26-oleoyloxy(d9) hexacosanoyl]-d-erythro-sphingosine (Cer[EOS] (d18:1/26:0/18:1(d9), MW 967.6) and N-[26-oleoyloxy hexacosanoyl]-d-erythro-sphingosine (Cer[EOS] (d18:1/26:0/18:1), MW 958.6) were purchased from Avanti Polar Lipids (Alabaster, USA). Deuterated cholesterol sulfate sodium salt (d7-CHS, MW 495.3) and Hexadecanoic-9,9,10,10,11,11,12,12,13,13,14,14,15,15,16,16,16-d17 acid (d17-PA, MW 273.5) were purchased from CDN Isotopes Inc., (Pointe-Claire, Canada). Stock solution of the internal standard (iSTD) mixture of deuterated compounds was prepared in 2-propanol with the following concentrations: d7-CHS 40 µM, d17-PA acid 80 µM, d31-Cer16:0 10 µM. d9-CER[EOS] and CER[EOS] mixture (2 µM each in Chloroform/methanol (2:1)) was introduced to confirm identification of O-acylceramides.

Maiellaro M., Bottillo G., Cavallo A, & Camera E. (2024). Comparison between ammonium formate and ammonium fluoride in the analysis of stratum corneum lipids by reversed phase chromatography coupled with high resolution mass spectrometry. Scientific Reports, 14, 40.

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Proteomic Sample Preparation Procedure

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DSSO, Dulbecco’s modified Eagle’s medium (DMEM), foetal bovine serum (FBS), l-glutamine, penicillin, streptomycin and phosphate-buffered saline (PBS) were obtained from Thermo Fisher Scientific (Les Ulis, France). Formic acid (FA), HPLC grade water, trifluoroacetic acid (TFA), acetonitrile (ACN), methanol, ethanol, acetone and trichloroacetic acid were all purchased from Biosolve (Dieuze, France). dl-Dithiothreitol (DTT), iodoacetamide (IAA), chloroform, dimethyl sulfoxide (DMSO), ammonium bicarbonate (AB), 4-(2-hydroxyethyl)piperazine-1-ethane sulfonic acid, N-(2-hydroxyethyl)piperazine-N-(2-ethane sulfonic acid) (HEPES), sodium chloride (NaCl) and magnesium chloride (MgCl₂) were obtained from Sigma-Aldrich. Tris was purchased from Bio-Rad (Steenvoorde, France). Extraction Illustra triplePrep Kit was from GE Healthcare. LysC/trypsin was obtained from Promega (Charbonnières-les-Bains, France). Amicon centrifugal filters and C18 ZipTip pipette tips were from Merck Millipore (Merck KGaA, Darmstadt, Germany).

Cardon T., Franck J., Coyaud E., Laurent E.M., Damato M., Maffia M., Vergara D., Fournier I, & Salzet M. (2020). Alternative proteins are functional regulators in cell reprogramming by PKA activation. Nucleic Acids Research, 48(14), 7864-7882.

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Optimized Mass Spectrometry Sample Preparation

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Water (HPLC and ULC/MS grade), ethanol, acetonitrile (ACN), trifluoroacetic acid (TFA), and xylene were obtained from Biosolve BV (Valkenswaard, The Netherlands).

α

-Cyano-4-hydroxycinnamic acid (CHCA), citraconic anhydride, and potassium sulfate were obtained from Sigma Aldrich (St. Louis, MI, USA). Hydrochloric acid fuming 37% was obtained from Supelco (Bellefonte, PA, USA). Basic N-glycan imaging kit was obtained from GlycoPath Inc. (Charleston, SC, USA).

Grgic A., Krestensen K.K, & Heeren R.M. (2023). Optimized protocol for MALDI MSI of N-glycans using an on-tissue digestion in fresh frozen tissue sections. Scientific Reports, 13, 2776.

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HPLC-PDA Analysis of Sildenafil Citrate

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Ethanol and mEthanol (HPLC grade) were purchased from Biosolve (Valkenswaard, The Netherlands). Formic acid was purchased from VWR Prolabo (Fontenay-Sous-Bois, France). Ammonium formate was procured from Sigma-Aldrich (St. Louis, USA). A sildenafil citrate reference standard was kindly donated by Pfizer (New York City, USA). The water, used during this study, was produced by a Milli-Q Gradient A10 system (Millipore, Billerica, USA) and will be referred to as Bwater^in the next paragraphs.
An Ammonium formate buffer (0.020 M) pH = 3 was prepared which served as aqueous phase during the HPLC-PDA analysis.
A reference solution of sildenafil citrate (0.1 mg mL -1 ) in Ethanol/water (50/50 v/v%) was prepared and analyzed under the same experimental conditions as the samples in order to determine the specific retention time.

Custers D., Krakowska B., De Beer J.O., Courselle P., Daszykowski M., Apers S, & Deconinck E. (2016). Testing of complementarity of PDA and MS detectors using chromatographic fingerprinting of genuine and counterfeit samples containing sildenafil citrate. Analytical and bioanalytical chemistry, 408(6).

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Characterization of Adulterated Botanical Supplements

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Reference material for Aristolochia fanghi, Ilex paraguariensis, Hoodia gordonii, and Garcinia cambogia was obtained from the American Herbal Pharmacopoeia (Scotts Valley, CA, USA), which authenticated the plant material through different macro- and microscopic techniques and provided a validation certificate confirming its identity.
Reagents such as m ethanol (HPLC grade), acetonitrile (HPLC grade), and ethanol (96% v/v) were obtained from Biosolve (Valkenswaard, The Netherlands). Formic acid (99.7%), hydrochloric acid solution (37 w/w%), and ammonia solution (25 w/w%) were all purchased from Merck (Darmstadt, Germany). A Millipore—MilliQ^® system (Billerica, MA, USA) was used to dispense milli-Q water. Lactose was also procured from Merck (Darmstadt, Germany).
All botanical supplements used for triturations and samples were selected from the samples seized by the Federal Agency for the Safety of the Food Chain (FASFC) (Brussels, Belgium) and sent to the laboratory to be tested for chemical adulteration. A choice for the botanical matrices to be used for triturations was made such that no concerned plant in this paper was listed on the label, and the supplement itself did not mention slimming as an indication. On the other hand, 12 samples were chosen, out of which 9 (samples 1–9) were slimming aids while 3 (samples 10–12) were potency enhancers.

Ranjan S., Adams E, & Deconinck E. (2023). Multidimensional Chromatographic Fingerprinting Combined with Chemometrics for the Identification of Regulated Plants in Suspicious Plant Food Supplements. Molecules, 28(8), 3632.

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Biocatalytic Oxidation Reaction Protocols

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3,3,5‐trimethylcyclohexanone (98%, Sigma‐Aldrich), sodium phosphite dibasic pentahydrate (> 98%, Sigma Aldrich), dichloromethane (Biosolve), hexadecane (99.5%, TCI), acetonitrile (Biosolve), 1,4‐dioxane (Sigma Aldrich), m ethanol (Biosolve), ethanol (Biosolve), 1,3‐dioxolane (Acros organics), and ethyl acetate (Biosolve) were used as received. 3‐Chloroperbenzoic acid (70%, Sigma Aldrich) was stored at –4 °C. β‐Nicotinamide adenine dinucleotide phosphate reduced tetrasodium salt NADPH (95%, Alfa Aesar) and β‐nicotinamide adenine dinucleotide phosphate disodium salt NADP⁺ (97%, Alfa Aesar) were stored at –20 °C and solutions, in buffer, were prepared fresh prior to use.

Delgove M.A., Elford M.T., Bernaerts K.V, & De Wildeman S.M. (2018). Application of a thermostable Baeyer–Villiger monooxygenase for the synthesis of branched polyester precursors. Journal of Chemical Technology and Biotechnology, 93(8), 2131-2140.

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Synthesis of Cationic Surfactant C16H33-[1,2-dimethyl-3-imidazolium]

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In total,
0.013 mol of 1-bromohexadecane (Sigma-Aldrich, 98.0%) and 0.016 mol
of 1,2-dimethylimidazole (Sigma-Aldrich, 97.0%) were dissolved in
a 50 mL mixture of ethanol (Biosolve, 99.9%) and acetonitrile (Biosolve,
99.9%) (1:5 molar ratio) and heated at 70 °C for 16 h under nitrogen
atmosphere. After evaporation of the solvents and addition of diethyl
ether (Biosolve, 99.5%), a white powder precipitated out of solution.
This solid product was filtered and dried in a vacuum oven at 50 °C
for 12 h. The purity of the organic surfactant C₁₆H₃₃-[1,2-dimethyl-3-imidazolium] was analyzed by ¹H, ¹³C, gHSQC, and gCOSY NMR after dissolution in CDCl₃ (Figures S1–S4). The product
yield was 75%.

Bolshakov A., van Diepen M., van Hoof A.J., Romero Hidalgo D.E., Kosinov N, & Hensen E.J. (2019). Hierarchically Porous (Alumino)Silicates Prepared by an Imidazole-Based Surfactant and Their Application in Acid-Catalyzed Reactions. ACS Applied Materials & Interfaces, 11(43), 40151-40162.

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Solvents and Reagents for Analysis

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M ethanol, ethanol, n-hexane, and xylene (ULC/MS-CC/SFC grade) were purchased from Biosolve Chimie (Dieuze, France). Red phosphorous standard, acetone, Entellan, and norhamane were purchased from Merck KGaA (Darmstadt, Germany).

Genangeli M., Heijens A.M., Rustichelli A., Schuit N.D., Micioni Di Bonaventura M.V., Cifani C., Vittori S., Siegel T.P, & Heeren R.M. (2019). MALDI-Mass Spectrometry Imaging to Investigate Lipid and Bile Acid Modifications Caused by Lentil Extract Used as a Potential Hypocholesterolemic Treatment. Journal of the American Society for Mass Spectrometry, 30(10), 2041-2050.

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