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Genomics spriworks ht kit

Manufactured by Beckman Coulter
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The Genomics SPRIworks HT Kit is a high-throughput DNA purification solution designed for automated sample preparation. It utilizes paramagnetic bead-based technology to efficiently purify and concentrate nucleic acids from various sample types, enabling seamless integration into automated workflows.

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Lab products found in correlation

Sureselectxt human all exon v5, by Agilent Technologies (2 mentions) Ampure xp bead, by Beckman Coulter (2 mentions) Biomek fxp, by Beckman Coulter (2 mentions) Hiseq 2000, by Illumina (2 mentions) Tapestation, by Agilent Technologies (2 mentions)

2 protocols using genomics spriworks ht kit

1

High-throughput Exome Sequencing Library Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA shearing was performed using Covaris instrument library construction a done on a Beckman Coulter Biomek FXP using Beckman Coulter Genomics SPRIworks HT Kit (https://www.beckmancoulter.com/wsrportal/wsr/research-and-discovery/products-and-services/next-gen-library-preparation/spriworks-ht/index.htm). First-round PCR (4–8 cycles) was performed using primers appropriate for Illumina (GA and HiSeq) sequencers, and clean-up steps with BC/Agencourt AMPure XP beads. Target capture utilized SureSelectXT Human All Exon V5 (Agilent Technologies) and supplied hybridization and associated reagents. Second-round PCR (10–16 cycles) used TruSeq index adapters. Library quality control was performed using a TapeStation (Agilent) and qPCR using Kapa standard curves. Sequencing was performed using Illumina HiSeq 2000 and 2500 sequencers in high-output (TruSeq SBS v3), rapid-high-output (HiSeq SBS v4) and rapid run (HiSeq Rapid SBS v1) modes. All runs were 100 nucleotide paired end reads, analyzed with on-board software RTA v1.18 and HCS v2.2.
Liu Y., Easton J., Shao Y., Maciaszek J., Wang Z., Wilkinson M.R., McCastlain K., Edmonson M., Pounds S.B., Shi L., Zhou X., Ma X., Sioson E., Li Y., Rusch M., Gupta P., Pei D., Cheng C., Smith M.A., Auvil J.G., Gerhard D.S., Relling M.V., Winick N.J., Carroll A.J., Heerema N.A., Raetz E., Devidas M., Willman C.L., Harvey R.C., Carroll W.L., Dunsmore K.P., Winter S.S., Wood B.L., Sorrentino B.P., Downing J.R., Loh M.L., Hunger S.P., Zhang J, & Mullighan C.G. (2017). THE GENOMIC LANDSCAPE OF PEDIATRIC AND YOUNG ADULT T-LINEAGE ACUTE LYMPHOBLASTIC LEUKEMIA. Nature genetics, 49(8), 1211-1218.
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2

High-throughput Exome Sequencing Library Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA shearing was performed using Covaris instrument library construction a done on a Beckman Coulter Biomek FXP using Beckman Coulter Genomics SPRIworks HT Kit (https://www.beckmancoulter.com/wsrportal/wsr/research-and-discovery/products-and-services/next-gen-library-preparation/spriworks-ht/index.htm). First-round PCR (4–8 cycles) was performed using primers appropriate for Illumina (GA and HiSeq) sequencers, and clean-up steps with BC/Agencourt AMPure XP beads. Target capture utilized SureSelectXT Human All Exon V5 (Agilent Technologies) and supplied hybridization and associated reagents. Second-round PCR (10–16 cycles) used TruSeq index adapters. Library quality control was performed using a TapeStation (Agilent) and qPCR using Kapa standard curves. Sequencing was performed using Illumina HiSeq 2000 and 2500 sequencers in high-output (TruSeq SBS v3), rapid-high-output (HiSeq SBS v4) and rapid run (HiSeq Rapid SBS v1) modes. All runs were 100 nucleotide paired end reads, analyzed with on-board software RTA v1.18 and HCS v2.2.
Liu Y., Easton J., Shao Y., Maciaszek J., Wang Z., Wilkinson M.R., McCastlain K., Edmonson M., Pounds S.B., Shi L., Zhou X., Ma X., Sioson E., Li Y., Rusch M., Gupta P., Pei D., Cheng C., Smith M.A., Auvil J.G., Gerhard D.S., Relling M.V., Winick N.J., Carroll A.J., Heerema N.A., Raetz E., Devidas M., Willman C.L., Harvey R.C., Carroll W.L., Dunsmore K.P., Winter S.S., Wood B.L., Sorrentino B.P., Downing J.R., Loh M.L., Hunger S.P., Zhang J, & Mullighan C.G. (2017). THE GENOMIC LANDSCAPE OF PEDIATRIC AND YOUNG ADULT T-LINEAGE ACUTE LYMPHOBLASTIC LEUKEMIA. Nature genetics, 49(8), 1211-1218.
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