O phthalaldehyde

In this work, the following reagents were used: Dulbecco’s Modified Eagle Medium (DMEM, low glucose-1 g/l for culturing fibroblasts), L-glutamine, 25 mM HEPES, sodium pyruvate, fetal bovine serum (FBS, ultra-low endotoxin content), Trypsin 0.25%, EDTA 0.02% in HBSS, Gentamicin (10 mg/ml) were purchased from Biowest (France). DMEM (high glucose-4.5 g/l for culturing rat cardiomyocytes) was purchased from OOO NPP PanEko (Russia). AlamarBlue^® Cell Viability Reagent; 5,5′,6,6′- tetrachloro-1,1′,3,3′- tetraethylbenzinidazolylcarbocyanine iodide (JC-1 dye); 2′, 7′- dihydrodichlorofluorescein diacetate dye (H2-DCFDA); propidium iodide (PI) were purchased from Molecular Probes Company (Eugene, USA). O-phthalaldehyde was purchased from Fisher Scientific Company (Loughborough, UK).

HPLC-grade solvents, ascorbic acid, DTPA, methylmonoarginine, o-phthalaldehyde, PCA, potassium hydroxide, and potassium phosphate were purchased from Fisher Scientific (Pittsburgh, PA, USA).