Rabbit polyclonal anti cleaved caspase 3 9661

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit polyclonal anti-cleaved caspase-3 (#9661) is a laboratory reagent that can be used to detect the presence of cleaved caspase-3 in biological samples. Caspase-3 is a key effector caspase involved in the apoptosis (programmed cell death) pathway. This antibody specifically recognizes the cleaved, active form of caspase-3.

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Lab products found in correlation

Anti β actin a1978, by Merck Group (1 mentions) Mouse monoclonal anti β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Adi spa 800, by Enzo Life Sciences (1 mentions) Rabbit polyclonal anti parp, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti cytochrome c, by BD (1 mentions) Rabbit polyclonal anti cleaved parp, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti hsp90, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Cleaved caspase-3 (3596 citations) Caspase-3 (2138 citations) Anti-cleaved caspase-3 (1212 citations) Anti-caspase-3 (744 citations) Rabbit anti-cleaved caspase-3 (360 citations) Rabbit anti-caspase-3 (146 citations) Rabbit polyclonal anti-cleaved caspase-3 (20 citations) Rabbit polyclonal anti-Caspase-3 (12 citations) Polyclonal anti-cleaved caspase 3 (7 citations) Polyclonal anti-caspase-3 (2 citations)

3 protocols using rabbit polyclonal anti cleaved caspase 3 9661

Antibody Characterization for Hypoxia Signaling

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Mouse monoclonal anti-CA9 antibody (NBP1-51691) was from Novus Biologicals (Centennial, CO). Rabbit monoclonal anti–HIF–1α (ab51608), rabbit polyclonal anti-SLC40A1 (ab58695) and rabbit polyclonal anti-ferritin light chain (ab69090) antibodies were from Abcam (Cambridge, UK). Rabbit polyclonal anti-ferritin heavy chain antibody (sc-25617) was from Santa Cruz Biotechnology (Dallas, TX). Mouse monoclonal anti-transferrin receptor-1 antibody (13–6800) was from Thermo Fisher Scientific (Pittsburg, PA). Rabbit monoclonal anti-IRP1/IRP2 (20272S/37135), rabbit monoclonal anti-LAMP1 (9091), rabbit monoclonal anti-LC3B (3868) and rabbit polyclonal anti-cleaved caspase-3 (9661) antibodies were from Cell Signaling (Danvers, MA). Mouse monoclonal anti-β-actin (A1978) was from Sigma Aldrich.

Li Z., Jiang L., Chew S.H., Hirayama T., Sekido Y, & Toyokuni S. (2019). Carbonic anhydrase 9 confers resistance to ferroptosis/apoptosis in malignant mesothelioma under hypoxia. Redox Biology, 26, 101297.

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Cloning and Characterization of HSP25 Mutants

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Wildtype (WT) mouse HSP25 (GenBank accession number, NM_013560.2) was polymerase chain reaction (PCR)-amplified, and the dimerization mutant HSP25 (C141A) was constructed by overlap extension PCR. The PCR fragments were digested with BamHI-EcoRI and ligated into p3x-Flag-Myc-CMV-26, yielding p3x-flag-HSP25 (WT) and p3x-flag-HSP25 (C141A). WT and dimerization mutant (C141A) HSP25 were cloned into p3x-Flag-myc-cmv-26 expression vector [21 (link)]. Goat polyclonal anti-HSP27 (sc-1049), goat polyclonal anti-HSP70 (sc-1060), mouse monoclonal anti-HSP90α/β (sc-13119), mouse monoclonal anti-HSF1 (sc-17757), rabbit-polyclonal anti-PKCδ (sc-213) and mouse monoclonal anti-β-actin (sc-47778) antibodies were purchased from Santa Cruz Biotechnology. Rabbit polyclonal anti-cleaved caspase-3 (#9661), rabbit polyclonal anti-cleaved PARP (#9541), and rabbit polyclonal anti-cleaved cytochrome c (#9661) antibodies were purchased from Cell Signaling (Beverly, MA, USA). Mouse monoclonal anti-cytochrome c (#556432, BD Biosciences) and rabbit polyclonal anti-PARP (#9542, Cell signaling) antibodies were used for immuneprecipitation. Mouse-monoclonal anti-HSP27 (ADI-SPA-800, Enzo) antibody was also used for immunohistochemistry.

Kim J.H., Jung Y.J., Choi B., Lee N.L., Lee H.J., Kwak S.Y., Kwon Y., Na Y, & Lee Y.S. (2016). Overcoming HSP27-mediated resistance by altered dimerization of HSP27 using small molecules. Oncotarget, 7(33), 53178-53190.

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Immunoblotting Antibody Panel for HSPs

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Goat polyclonal anti-HSP27 (sc-1049), goat polyclonal anti-HSP70 (sc-1060), mouse monoclonal anti-β-actin (sc-47778), and mouse monoclonal anti-HSP90 (sc-13119) were from Santa Cruz Biotechnology. Rabbit polyclonal anti-cleaved caspase-3 (#9661), and rabbit polyclonal anti-cleaved PARP (#9541) were from Cell Signaling. Rabbit monoclonal anti-HSF1 (ab52757) antibodies were from Abcam.

Choi B., Choi S.K., Park Y.N., Kwak S.Y., Lee H.J., Kwon Y., Na Y, & Lee Y.S. (2017). Sensitization of lung cancer cells by altered dimerization of HSP27. Oncotarget, 8(62), 105372-105382.

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