Panoramic viewer program

The tissue microarrays (TMAs) technique is widely recognized as a valid approach for preserving material in paraffin blocks, offering numerous benefits such as cost-effectiveness, consistent IHC reaction conditions, and efficient evaluation of IHC results, with only minor limitations (27 (link), 28 (link)). In our study 8 TMAs were prepared. Prior to performing TMAs blocks the histological slides stained with hematoxylin and eosin (HE) were obtained from whole samples of prostate and lymph nodes with detected prostate adenocarcinoma cells archived in the form of paraffin blocks (donor blocks). The slides were scanned using the Pannoramic Midi II histological scanner (3DHISTECH Ltd.). After that by using the Panoramic Viewer Program (3DHISTECH Ltd.), the representative areas from the entire sections where selected by uropathologist. In addition, to increase the representativeness of each case, 3 representative cores with a size of 1.5 mm from the donor block were selected and then transferred to the TMA ‘recipient’ block using the TMA Grand Master (3DHISTECH Ltd.). The TMA creation process is presented in Figure 1.

To determine the tumor volume of livers, HE stained sections were scanned by Panoramic Scan (3D Histech Ltd., Budapest, Hungary). The length and width of tumors in each section were determined with the help of Panoramic viewer program (3D Histech Ltd.) Tumor volume was calculated as V  =  (a²*b*π)/6 where ‘a’ refers to width (mm) and ‘b’ stands for length (mm).