Preparation of protein extracts and Western blot analysis were performed as described previously19 (link). Anti-GFP monoclonal antibody JL-8 (Clontech), anti-phospho-p38 MAPK monoclonal antibody D3F9 (Cell Signaling), anti-Hog1 polyclonal antibody y-215 (Santa Cruz), anti-phospho-p44/42 MAPK polyclonal antibody (Cell Signaling), anti-Mpk1 polyclonal antibody yN-19 (Santa Cruz), and anti-Mcm2 polyclonal antibody N-19 (Santa Cruz) were used. Detection was carried out by using a LAS-4000 (Fuji Film) with Immobilon Westren (Merck Millipore). Signal intensities were quantified by ImageQuant (GE Healthcare), and statistical analysis was performed with Excel (Microsoft).
Anti mpk1 polyclonal antibody yn 19
Manufactured by Santa Cruz Biotechnology
The Anti-Mpk1 polyclonal antibody yN-19 is a laboratory reagent used for the detection and analysis of the Mpk1 protein in various biological samples. It is a polyclonal antibody raised against a peptide corresponding to the N-terminal region of the Mpk1 protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti phospho p38 mapk monoclonal antibody d3f9, by Cell Signaling Technology (2 mentions)
Anti phospho p44 42 mapk polyclonal antibody, by Cell Signaling Technology (2 mentions)
Anti hog1 polyclonal antibody y 215, by Santa Cruz Biotechnology (2 mentions)
Anti mcm2 polyclonal antibody n 19, by Santa Cruz Biotechnology (2 mentions)
Las 4000, by Fujifilm (2 mentions)
Anti gfp monoclonal antibody jl 8, by Takara Bio (1 mentions)
Immobilon westren, by Merck Group (1 mentions)
Imagequant, by GE Healthcare (1 mentions)
Immobilon western, by Merck Group (1 mentions)
Odyssey imaging system, by LI COR (1 mentions)
Anti myc monoclonal antibody 9e10, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti mpk1 polyclonal antibody yn 19
1
Quantitative Protein Analysis by Western Blot
2
Western Blot Protein Analysis Workflow
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Preparation of protein extracts and Western blot analysis were performed as described previously9 (link). Anti-phospho-p38 MAPK monoclonal antibody D3F9 (Cell Signaling), anti-Hog1 polyclonal antibody y-215 (Santa Cruz), anti-phospho-p44/42 MAPK polyclonal antibody (Cell Signaling), anti-Mpk1 polyclonal antibody yN-19 (Santa Cruz), anti-Myc monoclonal antibody 9E10 (Santa Cruz) and anti-Mcm2 polyclonal antibody N-19 (Santa Cruz) were used. Detection was carried out by using a LAS-4000 (Fuji Film) with Immobilon Western (Merck Millipore) or the Odyssey Imaging Systems (LI-COR Biosciences). Signal intensities were quantified by the Odyssey Imaging Systems, and statistical analysis was performed with Excel (Microsoft).
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