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Ecorv and pmei restriction enzymes

Manufactured by New England Biolabs
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EcoRV and PmeI are restriction enzymes produced by New England Biolabs. Restriction enzymes are proteins that recognize and cleave specific DNA sequences, allowing for the manipulation and analysis of genetic material. EcoRV recognizes and cleaves the palindromic DNA sequence 'GATATC', while PmeI recognizes and cleaves the sequence 'GTTTAAAC'. These enzymes can be used in a variety of molecular biology applications, such as DNA cloning, genetic engineering, and DNA mapping.

Automatically generated - may contain errors

Lab products found in correlation

Rc210332, by OriGene (2 mentions) Rc203828, by OriGene (2 mentions) Hispeed plasmid maxi kit, by Qiagen (2 mentions)

Close spelling variants of this product

EcoRV restriction enzyme PmeI enzyme EcoRV enzyme Restriction enzymes EcoRV

2 protocols using ecorv and pmei restriction enzymes

1

Generating Mutant PRKACA and PRKAR1A

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Expression plasmids encoding human PRKACA and PRKAR1A were obtained from Origene (RC210332, NM_002730; RC203828, NM_002734). The Leu206Arg mutation was introduced into PRKACA using the QuikChange site-directed mutagenesis system (Stratagene), and validated by sequencing the complete coding region. Plasmids were prepared using the HiSpeed Plasmid Maxi Kit (Qiagen). The FLAG tag was deleted in the PRKAR1A construct for the IP experiment using EcoRV and PmeI restriction enzymes (New England Biolabs).
Goh G., Scholl U.I., Healy J.M., Choi M., Prasad M.L., Nelson-Williams C., Kuntsman J.W., Korah R., Suttorp A.C., Dietrich D., Haase M., Willenberg H.S., Stålberg P., Hellman P., Åkerström G., Björklund P., Carling T, & Lifton R.P. (2014). Recurrent activating mutation in PRKACA in cortisol-producing adrenal tumors. Nature genetics, 46(6), 613-617.
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2

Generating Mutant PRKACA and PRKAR1A

Check if the same lab product or an alternative is used in the 5 most similar protocols
Expression plasmids encoding human PRKACA and PRKAR1A were obtained from Origene (RC210332, NM_002730; RC203828, NM_002734). The Leu206Arg mutation was introduced into PRKACA using the QuikChange site-directed mutagenesis system (Stratagene), and validated by sequencing the complete coding region. Plasmids were prepared using the HiSpeed Plasmid Maxi Kit (Qiagen). The FLAG tag was deleted in the PRKAR1A construct for the IP experiment using EcoRV and PmeI restriction enzymes (New England Biolabs).
Goh G., Scholl U.I., Healy J.M., Choi M., Prasad M.L., Nelson-Williams C., Kuntsman J.W., Korah R., Suttorp A.C., Dietrich D., Haase M., Willenberg H.S., Stålberg P., Hellman P., Åkerström G., Björklund P., Carling T, & Lifton R.P. (2014). Recurrent activating mutation in PRKACA in cortisol-producing adrenal tumors. Nature genetics, 46(6), 613-617.
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