Goat anti mouse fitc
Goat anti-mouse FITC is a secondary antibody conjugated with the fluorescent dye fluorescein isothiocyanate (FITC). It is used to detect and visualize mouse primary antibodies in various immunoassay techniques, such as immunohistochemistry, flow cytometry, and Western blotting.
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13 protocols using goat anti mouse fitc
MHC Expression in Stem Cells
Quantifying Myelinating Oligodendrocytes in Rat Spinal Cord
Immunofluorescence Assay for Picornavirus Detection
Immunophenotyping of Adult Neural Stem Cells
Bystander Killing Effect of MW2821 and EV
Wound Healing Analysis in Diabetic Mice
For immunostaining, wound sections were dewaxed and rinsed with PBS. For antigen retrieval, tissue sections were treated with sodium citrate buffer at 95 °C for 10 min, followed by permeabilization with 0.1% Triton X-100 for 10 min. After blocking in PBST (PBS + 0.1% Tween 20) containing 2% BSA, tissue sections were incubated with 8-OHdG (Abcam; 1:200), anti-mouse CD31 (Abcam; 1:300), anti-CK10 (Abcam; 1:100), anti-mouse F4/80 (Abcam; 1:100), anti-mouse CD86 (Abcam; 1:100) or anti-mouse CD206 (Abcam; 1:100) antibodies for 8 h at 4 °C. Goat anti-mouse FITC (Abcam;1:200) and goat anti-rabbit TRITC secondary antibodies (Abcam; 1:400) were added for 2 h at room temperature. The fluorescence pictures were collected through a confocal fluorescence microscope (Leica SPII) with 20× magnification.
Quantitative Analysis of Oligodendrocyte Precursor Cells
and paraffin (Asia Pajohesh, Iran), serial sections (5-
μm thick) of the brain samples were prepared using a
microtome. After de-paraffinization and rehydration,
the slices were pre-treated using the method of heatinduced
epitope retrieval with sodium citrate buffer
(pH=6) and 1 mM EDTA buffer (pH=8) (Gibco,
USA) for 20 minutes, washed-out three times with
PBS (Gibco, USA). Then, the slices were incubated
with special antibodies against A2B5, a marker of
oligodendrocyte precursor cells (Abcam, USA) and
MOG, a myelin oligodendrocyte glycoprotein (Abcam,
USA), and then washed-out with PBS. Then, goat antimouse
-FITC (2 μg/ml, Abcam, USA) and rabbit antigoat-
FITC (Sigma-Aldrich, USA) were used as the
secondary antibody at room temperature for 1 hour.
Finally, nuclear counterstaining was conducted using
4, 6 Diamino-2-phenylindole, dilactate (DAPI) and
in order to quantitative analysis, the total numbers
of positive cells were counted in a minimum total of
200 cells per slide in six sections per sample using
.uorescence microscope (Olympus bx51, Japan).
Meanwhile, all IHC analyses were repeated at least
three times.
Assessing Myelination in Spinal Cord Transplants
anesthetized and fixing process was performed through
transcardially perfusion methods with ice-cold PBS and
4% paraformaldehyde (PFA) in PBS (pH=7.4). Rat spinal
cord was removed and postfixed in the same fixative at 4°C
overnight. Then, the sample was cryoprotected by 30%
sucrose (Sigma-Aldrich, USA) in PBS for 48-72 hours.
Subsequently, serial frozen sections (10 µ thick) of the
spinal cords were prepared using a microtome cryostat. In
order to evaluate the presence of myelin forming cells in
transplantation area, immunofluorescence technique was
done with primary antibodies include mouse monoclonal
anti-MBP (1:1000), mouse monoclonal anti-Olig2
(1:1000) and Goat anti-mouse FITC (1:2000, all purchased
from Abcam, UK) as secondary antibody. Finally,
after labeling the cell nucleus using 4', 6-Diamidino2-
Phenylindole, Dilactate (DAPI) cells were observed
using a fluorescence microscope, and immunopositive
cells was counted in a minimum total of 200 cells per
slide. Meanwhile, all immunofluorescence studies were
repeated at least twice.
Oligodendrocyte Differentiation Immunofluorescence
Immunofluorescence Staining of hASCs
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