The patients' medical histories were ascertained by a questionnaire. Vigorous physical activity and smoking were avoided for at least 30 minutes before the measurement of the exercise capacity and the HD session. Blood was drawn from an arteriovenous shunt just before starting the HD sessions to determine hemoglobin, serum albumin, blood urea nitrogen, creatinine (Cr), uric acid, calcium, phosphate, lipids (high‐ and low‐density lipoprotein cholesterol, and triglycerides), and C‐reactive protein; values were analyzed at commercially available laboratories (Daiichi Pure Chemicals, Tokyo, Japan and Wako Pure Chemical Industries, Osaka, Japan). Serum carnitine fraction levels were determined as described previously.12 Serum interleukin‐6 (R&D Systems, Minneapolis, MN, USA), fibroblast growth factor‐21 (FGF‐21) (R&D Systems), myostatin (Immundiagnostik AG, Bensheim, Germany), and decorin (Abcam plc, Cambridge, UK) were determined by enzyme‐linked immunosorbent assay according to the manufacturer's instruction. Changes of all data both before and after treatment were calculated using the following formula: (post data − pre data)/pre data × 100 (%).
Serum interleukin 6
Manufactured by R&D Systems
Sourced in United States
Serum interleukin‐6 is a recombinant human protein that can be used for research purposes. It is a cytokine that is involved in the inflammatory response and immune function.
Automatically generated - may contain errors
Lab products found in correlation
Fgf21, by R&D Systems (1 mentions)
Decorin, by Abcam (1 mentions)
Urine albumin, by Abcam (1 mentions)
Serum urea nitrogen, by BioAssay Systems (1 mentions)
Serum iron, by Sanofi (1 mentions)
Serum erythropoietin, by R&D Systems (1 mentions)
Hemavet 950, by Drew Scientific (1 mentions)
Serum amyloid a, by R&D Systems (1 mentions)
2 protocols using serum interleukin 6
1
Hemodialysis Protocol for Biomarker Analysis
2
Biomarker Measurement Methodology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Complete blood counts were measured by the Hemavet® 950 automated processor (Drew Scientific, Oxford, CT). Colorimetric methods were used to assay serum and urine phosphate (StanBio Labs, Boerne, TX), serum iron (Genzyme, Cambridge, MA), serum urea nitrogen (BioAssay Systems, Hayward, CA), and serum and urine creatinine (Crystal Chem, Elk Grove Village, IL). Commercially available enzyme-linked immunosorbent assays (ELISA) kits were used to measure urine albumin (Abcam, Cambridge, MA), serum erythropoietin (R&D Systems, Minneapolis, MN), serum amyloid A (R&D Systems), serum interleukin 1β (R&D Systems), serum interleukin 6 (R&D Systems), plasma parathyroid hormone (PTH) (Quidel, San Diego, CA), plasma C-terminal (total) fibroblast growth factor 23 (FGF23) (Quidel), and plasma intact FGF23 (Quidel). Whereas the total FGF23 assay detects both full-length, intact FGF23 and C-terminal FGF23 proteolytic fragments, the intact FGF23 assay detects only the full-length form. Percentage intact FGF23 was calculated as follows: ( [intact FGF23] / [total FGF23] ) × 100%. In-house ELISAs were used to measure serum hepcidin73 (link) and serum erythroferrone74 (link), as previously described.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!