Panexin nta

Manufactured by PAN Biotech

Sourced in United States, Germany

Panexin NTA is a lab equipment product offered by PAN Biotech. It is a buffer solution designed for the purification of proteins using immobilized metal-ion affinity chromatography (IMAC) techniques.

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Lab products found in correlation

Fetal calf serum (fcs), by Harvard Bioscience (2 mentions) Ts2 fl, by Nikon (1 mentions) Sw872, by American Type Culture Collection (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Venor gem, by Minerva Biolabs (1 mentions) Muse count viability assay kit, by Merck Group (1 mentions) Ht 29, by Thermo Fisher Scientific (1 mentions) Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions) Muse cell analyzer, by Merck Group (1 mentions)

Close spelling variants of this product

Panexin (2 citations)

4 protocols using panexin nta

Growth Factors and Kidney Tubulogenesis

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Example 4

The model can also be used to study how growth factors promote kidney tubulogenesis. In this example, the effect of growth factors in the serum was investigated. Panexin NTA from the company PAN-Biotech, a serum replacement containing no growth factors, was added to the medium in the same concentration as the reference serum (control), 10% fetal bovine serum (FBS). The tubulogenesis was quantified over the duration of the culture and the metabolic activity was measured after three weeks in the culture. As shown in FIG. 7, the lack of growth factors in the serum inhibits tubulogenesis. The metabolic activity of the cells was also increased by the addition of 10% FBS, as shown in FIG. 8.

US11597909B2. Method for the formation of renal tubules (2023-03-07). LEIBNIZ-INSTITUT FÜR POLYMER-FORSCHUNG DRESDEN E.V. [DE]. Inventors: Heather Weber [DE], Mikhail Tsurkan [DE], Valentina Magno [DE], Uwe Freudenberg [DE], Carsten Werner [DE].

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Neuronal Induction from Passage 4/5 ASCs

Cited 1 time

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The Passage 4/5 ASCs kept in complete medium was replaced with serum-free DMEM medium with 20 ng/ml of bFGF (known neuronal inducer) and 5% PAN serum replacement. The cells were maintained for 7 days in the same medium with periodical change of fresh medium for every 3 days. Later the bFGF containing medium was removed and washed with DPBS. Serum-free medium with 5% PAN serum replacement (Panexin NTA, PAN Biotech catalogue no. PO4-95700) was added to the cells. This temporal exposure (mimics in vivo exposure) of bFGF aids neuronal induction. The morphological changes were observed under Nikon Ts2FL phase contrast microscopy [22 (link)].

Martin C.A., Radhakrishnan S., Gómez Ribelles J.L., Trentz O.A., EAK N., Reddy M.S., Rela M, & Kalkura Subbaraya N. (2022). Adipose tissue derived stromal cells in a gelatin-based 3D matrix with exclusive ascorbic acid signalling emerged as a novel neural tissue engineering construct: an innovative prototype for soft tissue. Regenerative Biomaterials, 9, rbac031.

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Co-culture of Liposarcoma and TAFs

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The human liposarcoma cells, SW872, were purchased from the American Type Culture Collection (ATCC, Wesel, Germany; cell line CCI-121) and maintained in DMEM medium supplemented with 10% FCS, 1% penicillin (100 U/ml), 1% streptomycin (100 µg/ml) and 1% L-glutamine. The cells were cultured in a humidified atmosphere at 37°C with 5% CO₂ in 25 cm^² flasks. For co-culture, 5×10⁴ SW872 cells were seeded in 12-well co-culture plates (Corning Life Sciences, Acton, MA, USA) in DMEM medium supplemented with 10% complete chemically defined serum substitute PANEXIN NTA (PAN-Biotech), penicillin (100 U/ml), 1% streptomycin (100 µg/ml) and 1% L-glutamine. After 10 min Transwells with a pore size of 0.4 µm and 12 mm diameter (Corning Life Sciences) were inserted. Subsequently, 4×10⁴ TAFs were seeded in each Transwell insert and co-cultured with the SW872 cells for 48 h before the subsequent experiments were performed.

HARATI K., DAIGELER A., HIRSCH T., JACOBSEN F., BEHR B., WALLNER C., LEHNHARDT M, & BECERIKLI M. (2016). Tumor-associated fibroblasts promote the proliferation and decrease the doxorubicin sensitivity of liposarcoma cells. International Journal of Molecular Medicine, 37(6), 1535-1541.

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Cultivation of Adherent and Monocytic Cell Lines

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The adherent colon carcinoma cell line HT-29 (ATCC/LGC GmbH, Wesel, Germany) was cultivated in McCoy’s 5A medium (Gibco, Carlsbad, CA, USA) with either 10% fetal calf serum (FCS) (Biochrom AG, Berlin, Germany) for magnetic accumulation or cell death kinetics, or 10% Panexin NTA (PAN-Biotech GmbH, Aidenbach, Germany) whenever analyses of DAMPs or cytokines were planned, to minimize interactions with the detection. In preceding experiments, we confirmed that HT-29 cells showed similar behavior, no matter if cultivated in medium containing FCS or Panexin. The monocytic cell line THP-1 (TIB-202; American Type Culture Collection [ATCC], Manassas, VA, USA) was cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 2 mM glutamine, 10% FCS (Biochrom AG), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco^®). All cells were cultured in a cell culture incubator (INCOmed, Memmert, Schwabach, Germany) at 37 °C, 5% CO₂ and 95% humidified air. Cells were regularly checked for mycoplasma contamination using PCR kit Venor^®GeM (Minerva Biolabs GmbH, Berlin, Germany). Before every experiment, count and viability was analyzed using MUSE^® Count & Viability assay kit in MUSE cell Analyzer (Merck-Millipore, Billerica, MA, USA). A cell viability above 95% was required to start an experiment.

Ratschker T., Egenberger L., Alev M., Zschiesche L., Band J., Schreiber E., Frey B., Derer A., Alexiou C, & Janko C. (2020). Mitoxantrone-Loaded Nanoparticles for Magnetically Controlled Tumor Therapy–Induction of Tumor Cell Death, Release of Danger Signals and Activation of Immune Cells. Pharmaceutics, 12(10), 923.

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