Gelofusine

Manufactured by B. Braun

Sourced in Germany, United Kingdom, Denmark, China, Australia

Gelofusine is a colloid solution for intravenous infusion. It is an artificial plasma volume expander made from succinylated gelatin.

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Lab products found in correlation

Voluven, by Fresenius (2 mentions) Actrapid, by Novo Nordisk (2 mentions) Haes steril, by Fresenius (2 mentions) Mannitol, by Baxter (2 mentions) Trypsin edta solution, by Thermo Fisher Scientific (1 mentions) Gentamicin, by Sandoz (1 mentions) α mem, by Lonza (1 mentions) Lps from e coli o111 b4, by Merck Group (1 mentions) Dolethal, by Vetoquinol (1 mentions) Sprague dawley rats, by Janvier Labs (1 mentions) Ultraglutamin, by Lonza (1 mentions) Facsaria flow cytometer, by BD (1 mentions) Lethabarb euthanasia injection, by Virbac (1 mentions) Contour glucometer, by Bayer (1 mentions) Actrapid penfill, by Novo Nordisk (1 mentions) Cdi 500, by Terumo (1 mentions) Mannitol, by Fresenius (1 mentions) Probenecid, by Merck Group (1 mentions) Colchicine, by Merck Group (1 mentions) Sodium maleate, by Merck Group (1 mentions)

31 protocols using gelofusine

Endoscopic Resection and Coagulation of Colonic Angioectasia

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The mucosal lesion is resected by endoscopic mucosal resection (EMR). Submucosal injection is
made with normal saline or succinylated gelatin (“Gelofusine”; B. Braun, Crissier, Switzerland)
11 (link)
and adrenaline at a final concentration of 1:100 000. Inert dye
(methylene blue or indigo carmine) can be added to the solution to enhance delineation of the
colonic angioectasia margin. The mucosal part of the colonic angioectasia is resected en bloc
with fractionated electrocautery set to EndoCut Q, effect 3 (ERBE, Tübingen, Germany). The
underlying feeding vessel is then identified and cauterized with coagulation forceps using soft
coagulation, effect 4, 80 watts (ERBE, Tübingen, Germany). The defect is then closed with
endoscopic clips (
Fig. 1,
Fig. 2,
Video 1). Specimens were retrieved for histopathological examination (
Fig. 3). Snare tip soft coagulation with the same energy settings can be used for small flat vessels within the defect that are difficult to grasp.

Sriram N., Bar-Yishay I., Kumarasinghe P., Yusoff I., Segarajasingam D., Bourke M.J, & Raftopoulos S. (2019). Definitive therapy of colonic angioectasia by submucosal coagulation. Endoscopy International Open, 7(12), E1773-E1777.

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Fluid Usage Trends in ED and ICU

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Data were extracted from the pharmacy department’s purchase database from November 2014 until November 2018 for the ED and the ICU.
The following periods were used:

2015: 1 November 2014 – 31 October 2015; 2017: 1 November 2016 – 31 October 2017; 2018: 1 November 2017 – 31 October 2018,

2016: 1 November 2015 – 31 October 2016 was removed due to missing data.

The following fluids were chosen for review: 500 mL of starch-based colloid (Voluven, Fresenius Kabi, South Africa), 500 mL of gelatine-based colloid (Gelofusine, B Braun, South Africa), 1000 mL of 0.9% sodium chloride 1000 mL (sodium chloride 0.9%, Adcock Ingram, South Africa) (NS), 1000 mL of lactated Ringers (Ringer-Lactate Adcocare, Adcock Ingram, South Africa) (RL), 1000 mL of PlasmaLyte (SABAX Plasmalyte B, Adcock Ingram, South Africa) (PlasmB).
In our setting the administration of 20% albumin is a specialist led decision requiring motivation and seldom prescribed outside of the ICU. Given these conditions we opted to exclude albumin from our study.
Fluid amounts for crystalloids refer to litre bags and those for colloids refer to half litre bags.
All comparisons were between 2015 and 2018 except for cost, where the most recent period (2018) was compared with the average of the earlier pe-riods (2015/2017). All costs are noted in South African Rand in 1000s and taken to the first decimal place.

Jacobs K.A., Hindle L, & Omar S. (2021). A review of adult resuscitative fluid purchasing and usage trends at an academic tertiary hospital in Johannesburg. Anaesthesiology Intensive Therapy, 54(1), 56-61.

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Isolation and Characterization of Human Bone Marrow-Derived Mesenchymal Stromal Cells

Cited 2 times

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Human bone marrow (BM) was obtained from the iliac crest of healthy donors (N = 7) that were either subjects in a clinical trial or undergoing orthopaedic surgery for other than BM related problems. The unused surplus of mononuclear cell fraction obtained from BM after erythrocyte depletion using Gelofusine (B. Braun, Melsungen AG, Germany) sedimentation were supplied by Bioinova, Ltd. (Prague, Czech Republic) and processed as described previously^{59 (link),60 (link)}. Mononuclear cells were cultured in a complete culture medium (CCM) containing α-MEM (LONZA, Basel, Switzerland), 5% pooled PL (Bioinova, Ltd.), and 10 µg/ml gentamicin (Sandoz, Holzkirchen, Germany) at 37 °C, in a humidified atmosphere, containing 5% CO₂ with regular media changes twice a week. After reaching near-confluence, cells were harvested using the 0.05% Trypsin/EDTA solution (Life Technologies, Carlsbad, CA, USA) and reseeded onto a fresh plastic surface (Nunc, Roskilde, Denmark) at a density of 5 × 10³ cells/cm². Cells derived at passage 3 were used for the evaluation of immunophenotype, differentiation properties, gene expression and secretome profile in normoxic and hypoxic (5% O₂) conditions.

Petrenko Y., Vackova I., Kekulova K., Chudickova M., Koci Z., Turnovcova K., Kupcova Skalnikova H., Vodicka P, & Kubinova S. (2020). A Comparative Analysis of Multipotent Mesenchymal Stromal Cells derived from Different Sources, with a Focus on Neuroregenerative Potential. Scientific Reports, 10, 4290.

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Perfusate Comparison for Porcine Liver Preservation

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A bovine-derived gelatin-based solution (n = 6) (Gelofusine; B. Braun, Melsungen, Germany) plus pig washed red blood cells was compared with a human albumin (HA)/D-based solution (n = 6) (Steen; XVIVO, Denver, CO) plus pig washed red blood cells and WB (n = 6) as the perfusate for NEsLP machine. Porcine livers were retrieved after 30 minutes of WI (30min-WI) time. The 3 groups of livers were exposed to 2 hours of SCS while back table surgery, placing of cannulas, and blood preparation for perfusion were performed. After SCS, livers were perfused for 5 hours in a normothermic (37°C) setup of ex situ machine perfusion. Perfused livers were transplanted into recipient pigs with a follow-up time of 3 days. Perfusion setup in the 2 groups was similar to the recent human clinical trials.^{2 (link)–4 (link)} The only modification between the 3 groups was the use of a different perfusate solution (Steen, Gelofusine, or WB). An additional group of SCS (n = 6) was included in the study for comparison with the NEsLP groups. The total preservation time for the 4 groups was 7 hours.

Linares-Cervantes I., Kollmann D., Goto T., Echeverri J., Kaths J.M., Hamar M., Urbanellis P., Mazilescu L., Rosales R., Bruguera C., Oquendo F., Ganesh S., Adeyi O.A., Yip P., Selzner N, & Selzner M. (2019). Impact of Different Clinical Perfusates During Normothermic Ex Situ Liver Perfusion on Pig Liver Transplant Outcomes in a DCD Model. Transplantation Direct, 5(4), e437.

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Insulin-induced Glucose Response in Mice

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Prior to stimulation, mice were subjected to fasting for 4 h starting from 8:00 a.m. and then anesthetized using an IP injection of 7.5 mg pentobarbital sodium/100 g body weight for 15 min. Following the anesthesia, a single retroorbital injection of insulin (0.5 U/kg body weight; Actrapid, Novo Nordisk, Denmark) or a saline solution dissolved in Gelofusine (B. Braun, Denmark) was administered. Blood samples were collected from the tail vein immediately before and 15 min after the insulin or saline injections. The blood glucose levels were then analyzed using a glucometer (Bayer Contour; Bayer, Münchenbuchsee, Switzerland). The tissues were promptly dissected and snap-frozen, before being stored at −80 °C until further processing.

Henriquez-Olguin C., Meneses-Valdes R., Raun S.H., Gallero S., Knudsen J.R., Li Z., Li J., Sylow L., Jaimovich E, & Jensen T.E. (2023). NOX2 deficiency exacerbates diet-induced obesity and impairs molecular training adaptations in skeletal muscle. Redox Biology, 65, 102842.

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Pancreas Procurement and Preparation for EVNP

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The pancreases were all procured by one of the UK national organ procurement teams, using in situ perfusion with ice cold University of Wisconsin solution (CoStorSol^®; Bridge To Life, London, UK). Organs were packed and stored in preservation fluid and transported on ice. On arrival at the study centre, back bench preparation was performed by an experienced member of the transplant team as for clinical transplantation. The spleen was removed and splenic vessels ligated; arterial reconstruction was performed with a donor iliac Y graft to the splenic and superior mesenteric arteries using 5-0 polypropylene sutures. An arterial cannula was secured in the common iliac portion of the Y graft. The small bowel mesentery was over-sewn and excess peri-pancreatic tissue removed. A wide-bore catheter was inserted into the distal duodenum and secured with a purse-string suture. The pancreas was weighed on completion of back-bench preparation. Prior to EVNP the pancreas was flushed with cold Gelofusine (B. Braun, Sheffield, UK) and any obvious leaking vessels ligated with absorbable ligatures. The portal vein was left to drain freely back into the reservoir.

Barlow A., Hamed M., Mallon D., Brais R., Gribble F., Scott M., Howat W., Bradley J., Bolton E., Pettigrew G., Hosgood S., Nicholson M, & Saeb-Parsy K. (2015). Use of ex vivo normothermic perfusion for quality assessment of discarded human donor pancreases. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 15(9), 2475-2482.

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Machine Perfusion of Donor Livers

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The OrganOx metra device was used for NESLiP in all cases. The perfusate comprised group-compatible red cells suspended in either succinylated gelatin (Gelofusine, BBraun, n = 54) or 4.5% Human Albumin Solution (n = 149), to which boluses of heparin, amino acids, magnesium, calcium, meropenem, and fluconazole were added. Gas flow to the oxygenator is regulated automatically based on real-time analysis of the perfusate gas concentrations by an inbuilt Terumo module. Throughout perfusion, livers received infusions of heparin, sodium taurocholate, insulin, and epoprostenol, with a supplementary 20% dextrose infusion when perfusate glucose was <10 mmol/L.

Watson C.J., Gaurav R., Fear C., Swift L., Selves L., Ceresa C.D., Upponi S.S., Brais R., Allison M., Macdonald-Wallis C., Taylor R, & Butler A.J. (2022). Predicting Early Allograft Function After Normothermic Machine Perfusion. Transplantation, 106(12), 2391-2398.

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Kisspeptin Effects on Brain and Behavior

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In this double-blind, 2-way crossover, placebo-controlled randomized clinical trial, participants completed 2 study visits each (kisspeptin and placebo) at least 7 days apart (Figure 1A). Detailed methods are provided in the eMethods in Supplement 2. Participants had 2 intravenous cannulas sited for blood sampling and for kisspeptin or placebo administration. Blood sampling for hormonal measurements took place at 15-minute intervals from 30 minutes before commencement of the kisspeptin or placebo infusion to the end of the 75-minute infusion. At 0 minutes, a 75-minute infusion of kisspeptin-54 (1 nmol/kg/h) or placebo (using rate-matched 4% succinylated gelatin solution [Gelofusine; B. Braun]) commenced. Participants completed psychometric questionnaires before and toward the end of kisspeptin or placebo administration (eMethods in Supplement 2).
Between 30 and 60 minutes of the kisspeptin or placebo infusion, fMRI scanning was performed with participants completing 2 tasks as described next (and in the eMethods in Supplement 2).

Mills E.G., Ertl N., Wall M.B., Thurston L., Yang L., Suladze S., Hunjan T., Phylactou M., Patel B., Muzi B., Ettehad D., Bassett P.A., Howard J., Rabiner E.A., Bech P., Abbara A., Goldmeier D., Comninos A.N, & Dhillo W.S. (2023). Effects of Kisspeptin on Sexual Brain Processing and Penile Tumescence in Men With Hypoactive Sexual Desire Disorder: A Randomized Clinical Trial. JAMA Network Open, 6(2), e2254313.

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Haemorrhagic Shock Model in Rats

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Following the Institutional Animal Experimentation Ethics Committee approval (protocol number: 2019-06/03), a haemorrhagic shock model was formed with injury in 20 male 6-month-old Sprague-Dawley rats, with 5 rats in each group. At least 5 days prior to the experiment, 2 to 5 male rats per cage were allowed to have free access to food and water, followed by a 12-hour light-dark cycle at 24°C ± 1°C and 55% ± 5% humidity. Animals fasted 12 hours before the surgery. They were anaesthetized by intraperitoneal injection of a combination of ketamine hydrochloride (90 mg kg^–1) + xylazine hydrochloride (10 mg kg^–1). Thermal blankets were used to prevent heat loss in the animals. The animals that did not undergo haemorrhage were used as the sham group (group S). The others were randomised into 3 groups to have haemorrhage and intravenous 0.9% NaCl as a replacement fluid (group C: control group), haemorrhage and gelatine (Gelofusine®, B. Braun Melsungen AG, Melsungen, Germany) infusion as a replacement fluid (group G), and haemorrhage and HES (HES, B. Braun Medical, Melsungen, Germany) infusion as a replacement fluid (Group V). Gelatine required 0.04 g polygeline in 500 mL, HES was 130/0.4 in 500 mL, and a 6% concentration was used.

Saracoglu A., Saracoglu K.T., Ergun I.S., Yildirim M., Akca M., Demirtas C, & Tetik S. (2022). The effects of hydroxyethyl starch and gelatine on lung tissue and coagulation during the resuscitation of rats with traumatic haemorrhagic shock. Anaesthesiology Intensive Therapy, 54(5), 393-401.

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Glucose Uptake in Skeletal Muscle and Adipose Tissue

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Mice were fasted for 2 h before they were anesthetized with 75 mg pentobarbital/kg body weight and glucose uptake in skeletal muscle and adipose tissue was assessed in response to a single retro-orbital injection of ³H-2-deoxyglucose (³H-2-DG) [12.32 MBq/kg bodyweight (PerkinElmer, #NET549A005MC)] combined with insulin (0.75 U/kg bodyweight; Actrapid, Novo Nordisk, Denmark) or saline dissolved in Gelofusine (B. Braun, Denmark). Total blood glucose levels were determined by spectrophotometric measurements. A reaction mix consisting of 200 mM Tris-HCl, 500 mM MgCl₂, 5.2 mM adenosine triphosphate (ATP), 2.8 mM nicotinamide adenine dinucleotide phosphate (NADP) (#14469130, Roche) and 148 μg of a hexokinase and glucose-6-phosphate dehydrogenase mixture (#10737275001, Roche) with a pH of 7.4 was added to each sample. The samples were incubated at room temperature for 15 min and absorbance was measured (Hidex Sense, Hidex). Glucose uptake was determined 15 min after the injection as described (Treebak et al., 2010 (link)) by measuring the accumulation of ³H-2-DG in specific tissues. Radioactivity was determined in lysates by liquid scintillation counting (Hidex 300SL, Hidex) and related to tissue weight input.

Basse A.L., Dalbram E., Larsson L., Gerhart-Hines Z., Zierath J.R, & Treebak J.T. (2018). Skeletal Muscle Insulin Sensitivity Show Circadian Rhythmicity Which Is Independent of Exercise Training Status. Frontiers in Physiology, 9, 1198.

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