Omnic 9

Manufactured by Thermo Fisher Scientific

Sourced in United States

Omnic 9 software is a data collection and analysis tool for infrared spectroscopy. It provides functionality for controlling and acquiring data from compatible Thermo Scientific spectrometers.

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Lab products found in correlation

64 protocols using omnic 9

Silk Characterization by IR Spectroscopy

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Cocoons from bimolters and trimolters were chosen randomly and degummed as described previously [23 (link)]. The silk samples were then washed and air-dried at room temperature. IR spectroscopy was performed in the ATR mode using a Thermo Scientific Nicolet iN10 with a slide-on ATR objective lens. The spectra were collected in the range (650–4000 cm⁻¹) at a resolution of 8 cm⁻¹ with 256 coadded scans [24 (link)], and an applied ATR pressure of 75 psi. OMNIC 9 software (Thermo Scientific) was used to collect and process the spectral data. The background was collected before collecting the ATR spectra of the samples. Spectral data analysis, including baseline correction, deconvolution of the amide I bands, and peak fitting, was performed using OMNIC 9 software (Thermo Scientific) and PeakFit software (Seasolve, version 4.12) [25 (link),26 (link),27 (link)]. Each spectrum represents the mean of separate deconvolutions for at least 15 separate samples.

Guo K., Zhang X., Dong Z., Ni Y., Chen Y., Zhang Y., Li H., Xia Q, & Zhao P. (2020). Ultrafine and High-Strength Silk Fibers Secreted by Bimolter Silkworms. Polymers, 12(11), 2537.

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FT-Raman Spectral Analysis of Fractions

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FT Raman spectra of selected fractions (range 100–4000 cm⁻¹, 1000 scans, resolution 4 cm⁻¹) were recorded on Nicolet iS50 with the FT Raman module (ThermoFisher Scientific, Waltham, MA, USA), Nd:YAG laser (λ_ex = 1064 nm, power 500 mW), CaF₂ beam splitter and InGaAs detector. Vibration spectra were recorded and processed using Omnic 9.0 software (ThermoFisher Scientific, Waltham, MA, USA). The raw spectra were exported in ASCII format to Origin 6.0 software (OriginLab, Northampton, MA, USA) for further processing (smoothing, baseline correction), calculation of the average spectra.

Bleha R., Třešnáková L., Sushytskyi L., Capek P., Čopíková J., Klouček P., Jablonský I, & Synytsya A. (2022). Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure. Polymers, 14(2), 255.

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FTIR Analysis of Goat Bone Gelatin

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FTIR analysis of dry gelatin from Kacang goat bones was performed using Perkin Spectrum One (Shimadzu Prestige 21) according to the method of Renuka et al. [34 ]. The sample and KBr were mixed at a ratio of 1:100 for the preparation of the pellets to measure the absorbance spectrum from 650 to 4000 cm⁻¹. The spectral signal was collected in 36 scans at a resolution of 16 cm⁻¹. Spectral data analysis was performed using OMNIC 9.0 software (Thermo Fisher Scientific, USA).

Matulessy D.N., Erwanto Y., Nurliyani N., Suryanto E., Abidin M.Z, & Hakim T.R. (2021). Characterization and functional properties of gelatin from goat bone through alcalase and neutrase enzymatic extraction. Veterinary World, 14(9), 2397-2409.

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FTIR Spectroscopy of Chitin and Chitosan

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Fourier-transform infrared (FTIR) spectra were measured using the Nicolet iS50 FT-IR spectrometer (Thermo Fisher Scientific) equipped with a deuterated-triglycine sulfate detector and the Omnic 9.0 software (Thermo Fisher Scientific). For each sample, 64 scans were averaged with a spectral resolution of 4 cm⁻¹ in the middle region from 4,000 to 400 cm⁻¹. A diamond attenuated total reflectance sampling accessory was employed for solid-state measurements. The FTIR spectra of chitin and chitosan are shown in Supplementary Figure 1.

Tsvetkov Y.E., Paulovičová E., Paulovičová L., Farkaš P, & Nifantiev N.E. (2020). Synthesis of Biotin-Tagged Chitosan Oligosaccharides and Assessment of Their Immunomodulatory Activity. Frontiers in Chemistry, 8, 554732.

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FTIR Analysis of Laser-Engraved Wood

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Chemical changes were inspected in specimens irradiated at all the tree laser power and raster density values 10, 20, and 30 mm⁻¹. For this purpose, FTIR Analysis (Fourier Transform-Infrared Spectroscopy) was used. FTIR spectra of the engraved wood surfaces were recorded on a Nicolet iS10 FTIR spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), equipped with Smart iTR using an attenuated total reflectance (ATR) sampling accessory attached to a diamond crystal. The spectra were acquired by accumulating 64 scans at a spectral resolution of 4 cm⁻¹ in an absorbance mode from 4000 to 650 cm⁻¹ standardised using the baseline method. The obtained results were analysed using OMNIC 9.0 software (Thermo Fisher Scientific, Waltham, MA, USA). Measurements were performed on four replicates per a sample. Each specimen was measured on the radial surface.

Kúdela J., Kubovský I, & Andrejko M. (2022). Influence of Irradiation Parameters on Structure and Properties of Oak Wood Surface Engraved with a CO2 Laser. Materials, 15(23), 8384.

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Fourier Transform Infrared Analysis of Agars

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Fourier transform infrared analysis of two different agars was performed to determine the characteristic peaks spectroscopically with their functional groups using a NICOLET model iS50 FTIR (Thermo Fisher Scientific), using a diamond single reflection attenuated total reflectance, iS50 ATR (Thermo Fisher Scientific). The spectra appeared in the wavelength of 4000–400 cm⁻¹. The spectral data were collected and processed using OMNIC 9.0 Software (Thermo Scientific).

Mohibbullah M., Talha M.A., Baten M.A., Newaz A.W, & Choi J. (2023). Yield optimization, physicochemical characterizations, and antioxidant properties of food grade agar from Gracilaria tenuistipitata of Cox's Bazar coast, Bangladesh. Food Science & Nutrition, 11(6), 2852-2863.

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Spatially-Resolved Fungal-Mineral Cultivation Analysis

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After 120 h cultivation, the hydrated cultivated samples from both fungal-mineral
This article is protected by copyright. All rights reserved. Spatially-related μ-FTIR analysis was obtained at beamline BL01B1 of the National Center for Protein Science Shanghai (NCPSS). Spectra were recorded in reflectance mode using a Thermo Nicolet 6700 FTIR spectrometer and an infrared microscope with the following settings: aperture size 20 × 20 µm 2 , step size 10 × 10 µm 2 , resolution 4 cm -1 , spectral range 4000-650 cm -1 and 64 scans for mapping; aperture size 20 µm, step size 1 × 1 µm 2 , resolution 4 cm -1 , spectral range 4000-650 cm -1 and 128 scans for stacking of µ-FTIR (Du et al., 2019; Yu et al., 2019) . Both spectral mapping and the stacks of single FTIR spectra from µ-FTIR images was further processed using Omnic 9.0 software (Thermo Fisher Scientific Inc.) (Unger et al., 2013) . FTIR spectral background removal, normalization and automatic baseline correction were achieved using Omnic 9.0 software. The μ-FTIR data was processed by the RMieS-EMSC algorithm (v3) using MATLAB software (Bassan et al., 2010) .

Chi Z.L., Zhao X.Y., Chen Y.L., Hao J.L., Yu G.H., Goodman B.A, & Gadd G.M. (2021). Intrinsic enzyme-like activity of magnetite particles is enhanced by cultivation with Trichoderma guizhouense. Environmental microbiology, 23(2).

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FTIR Spectroscopy Analysis of Starch Structure

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FTIR spectra scanned from 400 cm⁻¹ to 4000 cm⁻¹ with a scanning resolution of 4 cm⁻¹ and 64 scans against the air as background were recorded by FTIR spectrometer (Nicolet iS50, Thermo Scientific, USA). Deconvolution analysis was performed using OMNIC 9.0 software (Visition9.0, Thermo Scientific, USA). The intensity ratio at 1047 cm⁻¹/1022 cm⁻¹ (R_1047/1022) was calculated for evaluating the ordered degree of the short-range ordered structure in starch [36] .

Liao L., Shen Y., Xie C., Zhang Y, & Yao C. (2024). Ultrasonication followed by aqueous two-phase system for extraction, on-site modification and isolation of microalgal starch with reduced digestibility. Ultrasonics Sonochemistry, 106, 106891.

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ATR-FTIR Spectroscopic Analysis of PSPHs

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The ATR-FTIR spectra of PSPHs (PSPH-Bro, PSPH-Alc, PSPH-Fla, PSPH-Pap, PSPH-Try) were determined using a Nicolet iS10 FTIR spectrophotometer (Thermo Fisher Scientific Corp., Waltham, USA). Each sample was placed on the crystal and the data was collected. The ATR-FTIR spectra was scanned 16 times with a spectral resolution of 2 cm⁻¹ and recorded at the wavenumbers between 4,000 to 400 cm⁻¹. The data was processed with the OMNIC software (Thermo Fisher Scientific Inc., OMNIC 9.2.86).

Lu D., Peng M., Yu M., Jiang B., Wu H, & Chen J. (2021). Effect of Enzymatic Hydrolysis on the Zinc Binding Capacity and in vitro Gastrointestinal Stability of Peptides Derived From Pumpkin (Cucurbita pepo L.) Seeds. Frontiers in Nutrition, 8, 647782.

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Cellulose Nanocrystal Structural Analysis

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The chemical structure of unprocessed, acid-treated, PFA-treated, bleached, and CNC extracted from RH was investigated using FT-IR. Samples were placed on KBr discs, and a Nicolet 6700 spectrometer (M/S Thermo Fisher Scientific Instruments, USA) was used to record spectral signals with an average of 32 scans, 4 cm⁻¹ resolution. OMNIC 9.2.86 (Thermo Fisher Scientific, Inc., USA) software was used to smooth the obtained spectra.

Vu A.N., Nguyen L.H., Tran H.C., Yoshimura K., Tran T.D., Van Le H, & Nguyen N.U. (2024). Cellulose nanocrystals extracted from rice husk using the formic/peroxyformic acid process: isolation and structural characterization. RSC Advances, 14(3), 2048-2060.

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