Antibodies used were as follows, with dilutions in parentheses: rabbit polyclonal NDUFB10 (ab196019, 1:2000) from Abcam; rabbit polyclonal SDHA (11998, 1:1000) from Cell Signaling Technology; rabbit polyclonal UQCRC2 (ab14745, 1:1000) from Abcam; rabbit polyclonal COXIV (4850, 1:10,000) from Cell Signaling Technology; rabbit polyclonal BNIP3 (HPA003015, 1:2000) from Cambridge Biosciences; rabbit polyclonal SDHC (PA5-79966, 1:1000) from Invitrogen; mouse monoclonal β-Actin (ab6276, 1:10,000) antibody from Abcam; donkey anti-rabbit (NA934, 1:1000) and anti-mouse (NA931, 1:1000) horseradish peroxidase (HRP)-linked secondary antibodies from VWR. Western blot signal intensity was measured per lane using ImageJ (NIH) analysis software. Sample protein band intensities were normalised to the load control protein β-Actin. Relative band intensities were calculated relative to the internal control sample.
Uqcrc2 ab14745
Manufactured by Abcam
UQCRC2 (ab14745) is an antibody product that can be used to detect the UQCRC2 protein, which is a component of the mitochondrial respiratory chain. The UQCRC2 protein is part of the ubiquinol-cytochrome c reductase complex, also known as complex III, which is involved in the electron transport chain and ATP generation in mitochondria. This antibody can be used in various research applications to study the UQCRC2 protein and its role in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Cox 4, by Cell Signaling Technology (1 mentions)
β actin ab6276, by Abcam (1 mentions)
Sod1 sc 11407, by Santa Cruz Biotechnology (1 mentions)
Cdk4 12790s, by Cell Signaling Technology (1 mentions)
Sc 1661, by Santa Cruz Biotechnology (1 mentions)
Pgc1α sc 13067, by Santa Cruz Biotechnology (1 mentions)
Sod2 sc 30080, by Santa Cruz Biotechnology (1 mentions)
β actin antibody, by Merck Group (1 mentions)
2 protocols using uqcrc2 ab14745
1
Quantitative Protein Analysis via Western Blot
2
Immunoblotting Protocol for Mitochondrial and Cell Cycle Proteins
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Immunoblotting was performed as described previously [7 (link)]. The following specific primary antibodies were used against p16 (sc-74400, 1:200; sc-1661, 1:500), SOD1 (sc-11407, 1:500), SOD2 (sc-30080, 1:200), ND4 (sc-20499-R, 1:200), PGC-1α (sc-13067, 1:200), PGC-1β (sc-373771, 1:200), PRC (sc-135516, 1:200), TFAM (sc-166965, 1:200), and Rb (sc-102, 1:500) obtained from Santa Cruz Biotechnology (Santa Cruz, CA). When the p16 antibodies were no longer available from Santa Cruz, we used a different antibody (NA29, 1:100) from EMD Millipore (Billerica, MA). The β-actin antibody was used at a 1:1000 dilution and obtained from Sigma-Aldrich (A-3853, St. Louis, MO, USA). The ATP5A (ab14746, 1;200), UQCRC2 (ab14745, 1:200), and tubulin (ab21058, 1:5000) antibodies were obtained from Abcam (Cambridge, MA). The SDHA antibody (MS 204M) was used at a 1:200 dilution and obtained from Mitoscience (Eugene, OR). The VDAC antibody (PAI-954A) was used at a 1:200 dilution and obtained from ABR Affinity Bioreagents (Golden, CO). The phospho-Rb (8180S, 1:1000), CDK4 (12790S, 1:1000), and CDK6 (3136S, 1:500) antibodies were obtained from Cell Signaling Technology (Beverly, MA).
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