Goat anti rabbit igg h l alexa fluor 488

The following items were purchased from the indicated sources: BCA protein assay kit was purchased from Dalian Meilun Biotechnology (Dalian, China). primary antibodies against E-cadherin (rabbit, #40860, Signalway Antibody, MD, USA), N-cadherin (rabbit, A5598, Bimake, Houston, TX, USA), Vimentin (rabbit, A5862, Bimake, Houston, TX, USA), phospho-ERK1+2 (rabbit, bs-1522R, Bioss, China), phospho-JNK1+2+3 (rabbit, bs-1640R, Bioss, Beijing, China), phospho-p38 MAPK (rabbit, bs-0636R, Bioss, Beijing, China), ERK1+ERK2 (rabbit, bs-2637R, Bioss, Beijing, China), JNK1+JNK2+JNK3 (rabbit, bs-2592R, Bioss, Beijing, China), p38 MAPK (rabbit, bs-0637R, Bioss, Beijing, China), GAPDH (rabbit, AP0066, Bioworld, MN, USA), Goat Anti-Rabbit IgG(H+L)/HRP (rabbit, bs-40295G-HRP, Bioss, Beijing, China) and Goat Anti Rabbit IgG (H&L)-Alexa Fluor 488 (rabbit, ImmunoWay, Plano, TX, USA).

Aladdin (Shanghai, China) supplied Ligustilide (C₁₂H₁₄O₂, molecular weight 190.24, purity ≥ 98%). TNF-α and primer were purchased from Thermo Fisher Biochemical Products (Beijing, China). TF antibodies were purchased from Abcam (Burlingame, CA, USA) and Proteintech Group, Inc. (Wuhan, China). The antibodies for p65, phosphor-p65, phosphor-IκBα, Akt, phosphor-Akt and β-actin were purchased from Affinity Biosciences (Cincinnati, OH, USA). Horseradish peroxidase (HRP)-conjugated secondary antibody and goat anti-rabbit IgG H&L (Alexa Fluor^® 488) were acquired from ImmunoWay Biotechnology (Plano, TX, USA). Lysis buffers and DAPI were purchased from Solarbio (Beijing, China). The PI3K inhibitor wortmannin was purchased from Beyotime Biotechnology (Shanghai, China). The RNA extraction kit and BCA kit were bought from CWBIO (Beijing, China). The TF activity assay kit was acquired from Abnova (Charles, MO, USA). The PVDF membrane was purchased from Millipore Corporation (Billerica, MA, USA). The FastKing gDNA Dispelling RT Super Mix kit was purchased from TIANGEN (Beijing, China).