Millex gv pvdf

No-carrier-added [¹⁸F]fluoride in cyclotron water (1.0–1.5 GBq, 1.5 mL) was trapped
on a preconditioned (10 mL H₂O) Waters Sep-Pak Accell Plus
Light QMA Carbonate (46 mg) cartridge. The cartridge was predried
with 10 mL of air, followed by elution of the [¹⁸F]fluoride
with a solution of TFA in H₂O/CH₃CN (18 μL
of TFA, 100 μL of H₂O, 400 μL of CH₃CN) into a glass V-vial containing Fe(acac)₃ (15.0 mg,
42.5 μmol). The elution efficiency of [¹⁸F]fluoride
off the QMA averages ∼80%. The V-vial was sealed, and the [¹⁸F]fluoride solution was heated at 80 °C for 10 min.
The vial was then opened and heated to 110 °C for 15 min until
dryness, resulting in a loss of ∼20% activity. A solution of
precursor 20 (1.0 mg, 1.6 μmol) in dry 1,4-dioxane
(50 μL) was added to the V-vial; then, the vial was sealed and
heated at 120 °C for 20 min. The reaction was removed from heat
and allowed to cool for a few minutes, then diluted with 500 μL
of HPLC eluent (60% CH₃CN), and filtered through a 0.22
μm filter (Millipore Millex-GV PVDF). The filtered solution
was injected onto HPLC, using the same HPLC conditions as described
in the General Radiochemistry Procedures section.