Rnasecure plant kit

Whole young leaves were collected from Pinus taeda, Pinus elliottii, and Pinus massoniana at the Forestry Science Institute of Yingde (Guangdong, China) and the Hongling seed orchard of Taishan (Guangdong, China). Collected leaves were stored at -20 °C until RNA extraction. Three 8-year-old P. taeda trees, with a similar growth state and free of pests and diseases were selected for sampling. The leaf buds, needles, twigs, trunk phloem, and roots were collected separately from each tree at the same time during the blooming period. Total RNA was extracted from different tissues of Pinus taeda using the TIANGEN RNAsecure Plant Kit (Beijing, China) following the manufacturer’s instructions. Sequencing libraries were prepared with insert sizes of 200 bp and sequenced using an Illumina HiSeq 4000 platform.

In this study, total RNA was extracted from A. oxyphylla using the RNAsecure Plant Kit (TIANGEN). The resulting RNA was assessed for purity using a NanoPhotometer^® spectrophotometer (IMPLEN, CA, USA) and for integrity using the RNA Nano 6000 Assay Kit on the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using the NEBNext^® Ultra™ RNA Library Prep Kit for Illumina^® (NEB, USA), following the manufacturer’s recommendations, with index codes added to attribute sequences to each sample. The total RNA content used was 1.5 μg. To cluster the index-coded samples, we employed the TruSeq PE Cluster Kit v3-cBot-HS (Illumina) and followed the manufacturer’s instructions. The library preparations were subsequently sequenced using the Illumina HiSeq platform, generating paired-end reads.