Water and tissue extracts were analysed using an Agilent Technologies (Stockport, UK) 7890A Gas Chromatography (GC) system interfaced with an Agilent 5975 series Mass Selective (MS) detector as described in [33 (link)].
7890a gas chromatography
Manufactured by Agilent Technologies
Sourced in United States
The 7890A gas chromatography system is a high-performance instrument designed for the separation, identification, and quantification of chemical compounds in a variety of sample types. It features a dual-channel configuration, multiple detectors, and advanced software for data analysis and reporting.
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Lab products found in correlation
Agilent 7000 triple quad system, by Agilent Technologies (2 mentions)
Spb 1 capillary column, by Merck Group (1 mentions)
J w db 5ms column, by Agilent Technologies (1 mentions)
Hp innowax, by Agilent Technologies (1 mentions)
Db ffap column, by Agilent Technologies (1 mentions)
1200 series hplc system, by Agilent Technologies (1 mentions)
Durabond db waxetr column, by Agilent Technologies (1 mentions)
Lambda 25, by PerkinElmer (1 mentions)
J w cp sil 88, by Agilent Technologies (1 mentions)
11 protocols using 7890a gas chromatography
1
GC-MS Analysis of Water and Tissue
2
GC-MS Analysis of Metabolites
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GC–MS analysis was performed on 7890A gas chromatography (Agilent Technologies, USA), equipped with an Agilent Technology GC autosampler 120 (PAL-LHX-AG12), coupled to an Agilent 7000 Triple Quad system (Agilent Technologies, USA). HP-5MS 30 m and 0.250 (mm) diameter fused silica capillary column (Agilent J&W Scientific, Folsom, CA, USA), chemically bonded with a 5% diphenyl and 95% dimethylpolysiloxane cross-linked stationary phase (0.25 mm film thickness) was used. The injection volume for GC–MS analysis was 1 μL of the derivatized sample extract. Helium was used as a carrier gas with a flow rate of 1 mL/min. The sample was injected in split mode. Injection temperature was 230 °C. The oven temperature was kept isothermal at 70 °C for 5 min, followed by 5 °C per min ramp to 310 °C. Ribitol was used as a quality control standard before and after every batch, and the mean value obtained from its retention time was 27.32 min with standard deviation of 0.02. A blank was run between the samples in order to remove the contamination. Electron ionization was used as an ionization source for GC–MS analysis at 70 eV. Data was acquired in the full scan mode from m/z 50 to 650 with a scan time of 0.5 s. Perfluorotributylamine (PFTBA) was used for mass calibration.
3
Quantitative Analysis of PCB-153 and 3-OH-PCB-153
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Quantitative analyses of PCB-153 and 3-OH-PCB 153 in sample extracts were carried out on a 7890A gas chromatography (GC) system (Agilent) equipped with a SPB®-1 capillary column ( film thickness; Supelco) and a 63Ni-micro electron capture detector (Agilent) as previously reported (Wu et al. 2011 (link)), with minor modifications and described below. Helium was used as carrier gas with a constant flow rate of . The injector and detector temperatures were and , respectively. The column temperature program was initially set as , held for 1 min, then gradually increased to by , increased to by , increased to a final temperature of by , and held there for 3 min. The PCB-153 and 3-OH-PCB-153 were identified by the retention time of their authentic standards. The relative retention time (RRT) of all analytes was within 0.5% of the RRT of the respective standard. PCB 153 and 3-OH-PCB 153 were quantified with the internal standard method as described (Kania-Korwel et al. 2007 (link)). Levels were corrected for recoveries below 100%.
4
GC-FID Analysis of Methylacetate
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MA was characterized by 7890A gas chromatography (Agilent Technologies, Santa Clara, CA, USA). Detector: FID; Column: HP-5 capillary column 30 m × 0.250 mm × 0.25 µm; carrier gas: N2; flow rate: 1.00 mL/min; injection volume: 0.8 µL; temperature: initial temperature 60 °C, at the rate of 10 °C/min, heating up to 200 °C; detector temperature 280 °C; injection port 280 °C; shunt ratio 10:1.
The standard method [24 ], GB28337-2012: “National Food Safety Standard for Food Additive Menthylate Acetate”, was used as a control method to detect MA content. Chromatographic conditions are as follows. Detector: FID; column: HP-5 capillary column 30 m × 0.320 mm × 0.25 μm; carrier gas: N2; Flow rate: 1.00 mL/min; injection volume: 0.2 μL; temperature: 220 °C constant temperature; detector temperature 250 °C; inlet 250 °C. The shunt ratio was 10:1.
The standard method [24 ], GB28337-2012: “National Food Safety Standard for Food Additive Menthylate Acetate”, was used as a control method to detect MA content. Chromatographic conditions are as follows. Detector: FID; column: HP-5 capillary column 30 m × 0.320 mm × 0.25 μm; carrier gas: N2; Flow rate: 1.00 mL/min; injection volume: 0.2 μL; temperature: 220 °C constant temperature; detector temperature 250 °C; inlet 250 °C. The shunt ratio was 10:1.
5
GC-MS Analysis of Fatty Acids
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Qualitative and quantitative analysis of the products were performed by GC-MS29 (link). To detect α-alkenes, an Agilent 7890A gas chromatography equipped with HP-INNOWAX (Agilent Technologies, Inc., cross-linked polyethylene glycerol, Santa Clara, CA, USA; 0.25 µm film thickness, 30 m by 0.25 mm) column was adopted. The column heating program was as follows: the initial temperature of oven was set to 40 °C for 4 min, then increased at a rate of 10 °C/min to 250 °C, and hold for 15 min. The α- and β-hydroxy fatty acids detection was carried out using the Agilent J&W DB-5 MS column (0.25 μm film thickness, 30 m by 0.25 mm). Furthermore, an Agilent 5975C MSD quadrupole mass spectrometer with a scan range from 50 to 500 m/z under electron ionization condition (70 eV) was coupled to the GC. The oven temperature was 50 °C initially and ramped up to 300 °C at the above mentioned rate, then 300 °C for 5 min. Quantification was performed using the corresponding authentic standard compounds and heptadecanoic acid as the internal standard.
6
Derivatization and GC-MS Analysis
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The dried extract of all the samples were derivatized subsequently by adding 50 μL methoxylamine hydrochloride in pyridine (15 μg/μL), vortexed and left for 2 hr at 35°C. Then BSTFA was added with 1% trimethylchlorosilane (TCMS) and placed at 70°Cfor 60 min to form trimethylsilyl (TMS) derivatives. GC-MS parameters were same as those reported in our previous paper26 (link). GC-MS analysis was performed using 7890A gas chromatography (Agilent technologies, USA), equipped with an Agilent Technology GC sampler 120 (PAL LHX-AG12) autosampler and coupled to a Agilent 7000 Triple Quad system (Agilent technologies, USA) and HP-5MS 30 m–250 mm (i.d.) fused-silica capillary column (Agilent J&W Scientific, Folsom, CA, USA), chemically bonded with a 5% diphenyl 95% dimethylpolysiloxane cross-linked stationary phase (0.25 mm film thickness) according to our previous report26 (link).
7
Acetic Acid Analysis by GC-FID
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Acetic acid analysis was performed on a 7890 A gas chromatography (GC) system (Agilent Technologies, Santa Clara, CA, USA) equipped with a DB-FFAP column (60 mm × 0.25 mm × 0.5 µm) and a flame ionization detector (FID). For the analysis, 3 mL of sample, 1 mL of internal standard (2-methyl butyric acid, 184 mg/L), 0.5 mL methanol, and 2.5 mL sulphuric acid (diluted 1:5 v/v) were placed in a headspace vial and closed with an aluminum crimp cap with PTFE/silicone septum. All GC measurements were conducted at least in triplicate. Detailed information about the parameters of the GC system has been published elsewhere [21 (link)].
8
Comprehensive Analytical Techniques
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iS10 infrared spectroscopy (PerkinElmer, USA), 7890A gas chromatography (Agilent, USA), alumina adsorption chromatography column (Shenyang Glass Instrument Factory), JA2003N electronic balance (Shanghai Jingke Instrument), and one-dimensional fire-flooded experimental device (self-developed by Northeast Petroleum University).
9
Analytical Methods for Biofuel Production
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Fermentation broth samples were analyzed for biomass growth, glycerol or sugar utilization, butanol, and solvent concentration. Biomass was determined by measuring optical density at 600 nm with appropriate dilution using a UV–visible spectrophotometer (Lambda-25, Perkin-Elmer, USA). 1,3-PDO, glycerol, glucose, and xylose were analyzed by a 1200 Series HPLC system (Agilent Technologies Inc.) equipped with an Aminex HPX-87H column (Bio-Rad, Richmond, CA, USA) and a Refractive Index Detector (RID). The samples were run at 75 °C with 0.6 mL/min eluent of 5 mM sulfuric acid. Biosolvents (i.e., acetone, ethanol, and butanol) and acids (i.e., acetic acid and butyric acid) were measured by a 7890 A gas chromatography (Agilent Technologies, USA) on a Durabond (DB)-WAXetr column (30 m × 0.25 mm × 0.25 µm; J&W, USA) equipped with a flame ionization detector (FID). The oven temperature was initially held at 60 °C for 2 min, increased to 230 °C at 15 °C/min, and held for 1.7 min. Helium was used as the carrier gas, with a flow rate of 1.5 mL/min. Five-point standard curves were obtained by running standard solutions containing biosolvents and acids.
10
Fatty Acid Methyl Ester Extraction
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Lyophilized cells (20 mg) were incubated with 2 ml of 1% methanol sulfate solution (v/v, containing 0.01% BHT) and 0.5 ml of heptadecanoic acid at 85°C for 2.5 h for fatty acid methyl ester (FAMEs). The reaction mixture was then shaken for 30 min. After cooling, 1 ml of 0.75% NaCl solution was added and the FAMEs were extracted using 2 ml of chromatographic-grade hexane. The supernatant was collected via centrifugation at 5,000 × g for 5 min, and the samples were subsequently nitrogen blown using a pressurized nitrogen concentrator. The sample was filtered through a disposable microporous filter (13 mm × 0.22 μm). FAMEs were performed by 7890A gas chromatography (Agilent, USA). All measurements were performed in triplicates.
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