Sds page gel rapid preparation kit

Manufactured by Beyotime

Sourced in China

The SDS-PAGE gel rapid preparation kit is a laboratory equipment designed to facilitate the quick and efficient preparation of SDS-PAGE gels. It provides the necessary components and step-by-step instructions to streamline the gel casting process, enabling researchers to consistently produce high-quality gels for their electrophoresis experiments.

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Lab products found in correlation

4 protocols using sds page gel rapid preparation kit

Protein Extraction and Immunoblotting

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Referring to the previous description (32 (link)), protein extraction and immunoblotting were performed. The antibodies directed against the following proteins, including PI3K, p-PI3K, AKT, p-AKT, and β-actin were used, and their directions are shown in Table 1. HRP-conjugated secondary antibodies (1:5,000, GeneTex, Irvine, CA, USA) were also used. Other materials consisted of electrophoresis and membrane transfer device (Bio-Rad), RIPA lysate (Beyotime), protease inhibitor (cocktail) (Roche), ECL chemiluminescence substrate Kit (Biosharp, Hefei, China), defatted milk powder (Wondersun, Harbin, China), protein marker (Bio-Rad), BCA kit (Beyotime), and SDS-PAGE gel rapid preparation kit (Beyotime). Blotting was captured by Molecular Imager ChemiDocTM XSR+ Gel Imaging System (Bio-Rad) and analyzed using ImageJ software (NIH). In semiquantitative analysis of the target protein, each sample was normalized to β-actin.

He X.Y., Xu Y., Xia Q.J., Zhao X.M., Li S., He X.Q., Wang R.R, & Wang T.H. (2021). Combined Scutellarin and C18H17NO6 Imperils the Survival of Glioma: Partly Associated With the Repression of PSEN1/PI3K-AKT Signaling Axis. Frontiers in Oncology, 11, 663262.

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Synthesizing Zinc-Imidazole Photosensitizer

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2-methylimidazole (C₄H₆N₂, 98%), and zinc nitrate hexahydrate (N₂O₆Zn·H₂O, AR, 99%) were purchased from Aladdin (Shanghai, China). Methanol was acquired from Shanghai Lingfeng Chemical Reagent Co., Ltd. Monomethorphyrin (HMME) was purchased from Xi’ an Ruixi biological technology Co., Ltd. 1,3-diphenylisobenzofuran (DPBF) was gained from Sigma-Aldrich Co. Ltd., USA. SDS-PAGE Gel Rapid Preparation Kit, 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), QuickBlock™ blocking agent and transferred to polyvinylidene difluoride (PVDF) membrane were acquired from Beyotime Co. Ltd., China. 2× Rapid Taq Master Mix, ChamQ SYBR qPCR Master Mix, T7 Endonuclease I, Ultra GelRed nucleic acid stain (10,000×), BCA protein quantification kit, 100 bp DNA Ladder, RNA-easy isolation reagent, 2× Phanta Max Master Mix (Dye Plus), FastPure cell/tissue total RNA isolation kit V2 and HiScript II 1st strand cDNA synthesis kit were acquired from Vazyme Co., Ltd. Dimethyl sulfoxide (DMSO) was acquired from Macklin (Shanghai, China). Pierce™ ECL Western Blotting Substrate was purchase from Thermo Fisher Co., Ltd. Mouse IL-2, IL-12p70 ELISA kits were acquired from Multiscience, China.

Yu J., Zhou B., Zhang S., Yin H., Sun L., Pu Y., Zhou B., Sun Y., Li X., Fang Y., Wang L., Zhao C., Du D., Zhang Y, & Xu H. (2022). Design of a self-driven probiotic-CRISPR/Cas9 nanosystem for sono-immunometabolic cancer therapy. Nature Communications, 13, 7903.

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Nanoparticle Drug Delivery System Development

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TET and dauricine were purchased from Dalian Meilun Biotechnology Co., Ltd. PLGA (50/50, molecular weight 30000) was purchased from Jinan Biotech Co., Ltd. F-68 was purchased from BASF. SDS-PAGE gel rapid preparation kit and DiO (cell membrane green fluorescent probe) were purchased from Biyuntian Biotechnology Co., Ltd. Phosphotungstic acid (pH 6.5) and carbon support copper mesh (230 mesh) were purchased from Beijing Zhongjing Keyi Technology Co., Ltd. Dialysis bag (COMW = 3500 Da) was purchased from United States for carbonization. Polycarbonate film was purchased from Whatman Company of the United Kingdom. ADR was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. Nile Red was purchased from Shanghai Maclean Biochemical Technology Co., Ltd. RPMI 1640 medium, DMEM medium and fetal bovine serum (FBS) were purchased from Gibco, USA. CCK-8 kit was purchased from Tongren Chemical Research Institute, Japan. Dimethyl sulfoxide (DMSO) was purchased from Sigma, USA. Methanol and acetonitrile were HPLC grade, other reagents were analytical grade.

Que X., Su J., Guo P., Kamal Z., Xu E., Liu S., Chen J, & Qiu M. (2019). Study on preparation, characterization and multidrug resistance reversal of red blood cell membrane-camouflaged tetrandrine-loaded PLGA nanoparticles. Drug Delivery, 26(1), 199-207.

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Comprehensive Molecular Analysis Protocol

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Total RNA extraction reagent TRIzol, RNA reverse transcription kit, polymerase chain reaction (PCR) amplification kit, and primers were purchased from Invitrogen (USA). The Nox4 antibody was purchased from Abcam and Nox2 antibody was purchased from Boster Bioengineering Co., Ltd. The GAPDH antibody and horseradish peroxidase (HRP) was purchased from Shanghai Bioengineering Co., Ltd. The MSF (100 mM), SDS‐PAGE protein loading buffer (5×), SDS‐PAGE gel rapid preparation kit, pre‐stained protein standard molecular weight, SDS‐PAGE electrophoresis buffer, western semi‐dry transfer solution, hypersensitive ECL chemiluminescence kit, BeyoRed DNA loading buffer (6×), and Diethyl pyrocarbonate (DEPC) treated water were purchased from Biyuntian Biotechnology Research Institute. Ethidium bromide (EB) was purchased from Amresco (USA). Acetylcholine (ACh) and phenylephrine (Phe) were purchased from Hefei Bomei Biotechnology Co., Ltd.

Zhou H., Wang H., Shi N, & Wu F. (2020). Potential Protective Effects of the Water‐Soluble Chinese Propolis on Hypertension Induced by High‐Salt Intake. Clinical and Translational Science, 13(5), 907-915.

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