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Immulite 2500 analyzer

Manufactured by Siemens
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Sourced in Germany

The Immulite 2500 analyzer is a fully automated, random-access immunoassay system designed for the quantitative determination of various analytes in patient samples. It utilizes chemiluminescent technology to perform a range of immunoassays, providing accurate and reliable results. The core function of the Immulite 2500 is to analyze patient samples and generate quantitative test results for clinical diagnosis and monitoring purposes.

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Lab products found in correlation

Immulite 200 xpi analyzer, by Siemens (2 mentions) Cell dyn sapphire, by Abbott (1 mentions) Vista analyzer, by Siemens (1 mentions) Cobas 8000 modular analyzer, by Roche (1 mentions) Anti igg, by Merck Group (1 mentions) Synaptophysin, by Zymo Research (1 mentions) E601 analyzer, by Roche (1 mentions) Immulite 200 xpi, by Siemens (1 mentions)

6 protocols using immulite 2500 analyzer

1

Comprehensive Metabolic Evaluation Protocol

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Patients underwent routine hematological, immunological, and biochemical testing after an overnight fast. Glucose and lipid values were determined by a Vista analyzer (Siemens Healthcare Diagnostics, Deerfield, IL). Insulin was analyzed by a chemiluminescence immunoassay on a Siemens Immulite 2500 analyzer. HbA1c was measured by high-performance liquid chromatography. Complete blood count and differential was determined by Cell-Dyn Sapphire (Abbott Diagnostics, Santa Clara, CA). Total testosterone was measured by chemiluminescence immunoassay on a Siemens Immulite 2500 analyzer. The presence of the insulin receptor antibody was confirmed by measuring the ability of patients’ sera to immunoprecipitate recombinant human insulin receptors, as described previously (9 (link)). Titers were based on semiquantitative assessment of Western blot band intensities compared with a positive control and were scored by one observer (R.K.S.).
Klubo-Gwiezdzinska J., Lange M., Cochran E., Semple R.K., Gewert C., Brown R.J, & Gorden P. (2018). Combined Immunosuppressive Therapy Induces Remission in Patients With Severe Type B Insulin Resistance: A Prospective Cohort Study. Diabetes Care, 41(11), 2353-2360.
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2

Biomarker Detection Protocols in Venous Blood

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Venous whole blood samples were collected in tubes containing either ethylenediaminetetraacetic acid (EDTA) or lithium heparin. Copeptin, MR-proANP and pro-ADM were determined in EDTA plasma by Time-Resolved Amplified Cryptate Emission (TRACE) technology on the KRYPTOR analyzer (BRAHMS, Henningsdorf, Germany).
Plasma levels of PCT and NT-proBNP were measured by electrochemiluminescence immunoassays on a COBAS 8000 modular analyzer (Roche Diagnostics, Rotkreuz, Switzerland). IL-6 levels were analyzed using a chemiluminescence enzyme immunoassay on an Immulite 2500 analyzer (Siemens Healthcare Diagnostics, Zurich, Switzerland) and cystatin C was measured by immuno-nephelometry on a Behring nephelometer system (Dade Behring, Dudingen, Switzerland).
Plasma concentrations of AST, ALT, AP, bilirubin and CRP, were measured on a COBAS 8000 modular analyzer (Roche Diagnostics, Rotkreuz, Switzerland).
Zoller B., Spanaus K., Gerster R., Fasshauer M., Stehberger P.A., Klinzing S., Vergopoulos A., von Eckardstein A, & Béchir M. (2014). ICG-liver test versus new biomarkers as prognostic markers for prolonged length of stay in critically ill patients - a prospective study of accuracy for prediction of length of stay in the ICU. Annals of Intensive Care, 4, 19.
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3

Quantification of Steroid Hormones by LC-MS/MS

Cited 2 times
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We quantified 23 steroids by LC-MS/MS, including three unconjugated ∆5-steroids, four steroid sulfates, 14 ∆4-steroids, and three 3α,5α-reduced steroids. Steroid extraction and quantitation was carried out as previously described (16 (link), 17 (link)). The 3α,5α-reduced steroids (Supplemental Table 1) were derivatized with 50 µL of 1 M ammonium hydroxide and 100 µL of 1 M hydroxylamine hydrochloride, as described for Δ5 steroids (16 (link), 22 (link)).
Plasma ACTH was analyzed at the NIH Clinical Center (Bethesda, MD) by chemiluminescent immunoassay on a Siemens Immulite 200 XPi analyzer or on a Siemens Immulite 2500 analyzer (before 2012; Munich, Germany). The assay had a sensitivity of 5 pg/mL, reference range of 0 to 46 pg/mL, and a within-run coefficient of variation of 1.61% to 3.36% at a mean concentration of 33.97 and 466 pg/mL, respectively, and a day-to-day variability of 3.09% to 4.16% at a mean concentration of 34 and 469 pg/mL, respectively. Plasma renin activity was analyzed by LC-MS/MS and inhibin B by enzyme-linked immunosorbent assay at the Mayo Medical Laboratories (Rochester, MN).
Turcu A.F., Mallappa A., Elman M.S., Avila N.A., Marko J., Rao H., Tsodikov A., Auchus R.J, & Merke D.P. (2017). 11-Oxygenated Androgens Are Biomarkers of Adrenal Volume and Testicular Adrenal Rest Tumors in 21-Hydroxylase Deficiency. The Journal of Clinical Endocrinology and Metabolism, 102(8), 2701-2710.
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4

Adrenal Gland Histopathology Analysis

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The adrenal glands from all patients were processed as per routine histopathological procedures for diagnostic purposes. Hematoxylin and eosin (H&E)- stained slides and other appropriate IHC stainings (for example, inhibin-A dilution 1:100, Biogenex, Inc.; synaptophysin, dilution 1:100, Zymed, Inc) were reviewed. Detailed analysis of the pathology of these patients has been reported elsewhere6 . IHC on unstained slides from breast tumor tissue for patient CAR 615.05 was performed in collaboration with Histoserve, Inc.(Germantown, MD) using standard procedures. The primary antibody used was rabbit Anti-Ca, sc 903 (Santa Cruz Biotechnology, Santa Cruz, CA) and the secondary antibody was rabbit polyclonal Anti- IgG (Sigma-Aldrich Inc., St. Louis, MO). ACTH was measured using the chemiluminescence immunoassay on an Immulite 200 XPi analyzer (Siemens, Malvern, PA) Serum cortisol was measured using the chemiluminescence immunoassay on Siemens Immulite 2500 analyzer. (Siemens, Malvern, PA).
Lodish M.B., Yuan B., Levy I., Braunstein G.D., Lyssikatos C., Salpea P., Szarek E., Karageorgiadis A.S., Belyavskaya E., Raygada M., Faucz F.R., Izatt L., Brain C., Gardner J., Quezado M., Carney J.A., Lupski J.R, & Stratakis C.A. (2015). Germline PRKACA amplification causes variable phenotypes that may depend on the extent of the genomic defect: molecular mechanisms and clinical presentations. European journal of endocrinology / European Federation of Endocrine Societies, 172(6), 803-811.
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5

Hormonal Biomarker Measurement Protocol

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Fasting blood was drawn in the morning before taking medication. Hormones were analyzed at the National Institutes of Health Clinical Center (Bethesda, MD) unless noted otherwise. Testosterone, androstenedione, 17-hydroxyprogesterone (17-OHP) and dehydroepiandrosterone (DHEA) were measured by liquid chromatography-tandem mass spectrometry (Mayo Medical Labs, Rochester, MN); dehydroepiandrosterone sulfate (DHEAS) by automated chemiluminescent immunometric assay (Mayo Medical Laboratories) until 2011 and by chemiluminescence immunoassay on Siemens Immulite 2500 analyzer (NIH) after 2011; parathyroid hormone (PTH) by electrochemiluminescence immunoassay on Roche Cobas e601 analyzer; and 25-hydroxyvitamin D by electrochemiluminescence binding assay on Roche Cobas.
El-Maouche D., Collier S., Prasad M., Reynolds J.C, & Merke D.P. (2014). Cortical Bone Mineral Density in Patients with Congenital Adrenal Hyperplasia due to 21-Hydroxylase Deficiency. Clinical endocrinology, 82(3), 330-337.
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6

Comprehensive Hormonal Evaluation Protocol

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Fasting morning hormonal evaluation included measurement of serum 17-OHP, androstenedione, free and total testosterone, cortisol, ACTH, plasma renin activity, aldosterone, LH, and plasma fractionated metanephrines and catecholamines. Additionally, a fasting lipid panel, glucose, and serum insulin level were obtained. Plasma ACTH was analyzed at the NIH Clinical Center (Bethesda, MD) by chemiluminescent immunoassay on a Siemens Immulite 200 XPi analyzer or on a Siemens Immulite 2500 analyzer (before 2012; Munich, Germany). Adrenal biomarkers were measured by liquid chromatography-tandem mass spectrometry (prior to 2012: Mayo Medical Labs, Rochester, MN; otherwise NIH Clinical Center, Bethesda, MD).
Normal range for lab levels had slight variations with time. Upper limit of normal (ULN) for labs (highest during the study duration) were: 17-OHP (8.4 nmol/L), ACTH (13.2 pmol/L), Aldosterone (1.23 nmol/L), Androstenedione (10.8 nmol/L), Cortisol (690 nmol/L), Plasma free metanephrine (0.32 nmol/L), Renin (4.3 mcg/mL/hr).
El-Maouche D., Hannah-Shmouni F., Mallappa A., Hargreaves C.J., Avila N.A, & Merke D.P. (2019). Adrenal Morphology and Associated Comorbidities in Congenital Adrenal Hyperplasia. Clinical endocrinology, 91(2), 247-255.
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