Anti mouse igg fitc

Manufactured by Jackson ImmunoResearch

Sourced in United States

Anti-mouse IgG-FITC is a secondary antibody conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate). It is designed to detect and bind to mouse immunoglobulin G (IgG) molecules, allowing visualization and quantification in various immunological applications.

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Lab products found in correlation

Anti mouse igg cy3, by Jackson ImmunoResearch (2 mentions) Cy3 anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Anti mouse igm fitc, by Jackson ImmunoResearch (2 mentions) Nf200, by Merck Group (1 mentions) Cryostat, by Leica camera (1 mentions) Image pro plus software, by Media Cybernetics (1 mentions) Eclipse 50i, by Nikon (1 mentions) C1 confocal microscope, by Nikon (1 mentions) Mouse anti plzf, by Santa Cruz Biotechnology (1 mentions) Rabbit anti sycp3, by Abcam (1 mentions) Goat anti rabbit igg h l hrp, by Bioworld Technology (1 mentions) Fitc anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Rabbit anti mvh, by Abcam (1 mentions) Mouse anti β actin, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse igg hrp, by Abmart (1 mentions) Mouse anti mlh1, by BD (1 mentions) Anti mouse igg, by BioLegend (1 mentions) Impact 2 test, by IDEXX (1 mentions) Anti goat igg fitc, by Jackson ImmunoResearch (1 mentions) Pab7154, by Abnova (1 mentions)

10 protocols using anti mouse igg fitc

Immunohistochemistry of Sciatic Nerve

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Animals were sacrificed after the behavioral test for immunohistochemistry. The sciatic nerve was collected according to a previously described method [20 (link)]. The sciatic nerves were dissected and immediately immersed in a solution consisting of 4% paraformaldehyde in 100 mM PBS overnight. For cryoprotection, fixed tissue was immersed in a solution consisting of 30% sucrose in PBS. Longitudinal sections 20 μm thick were cut using a cryostat (Leica, Nussloch, Germany). The tissue slides were incubated overnight at 4°C with primary antibody to neurofilament protein (NF-200; Sigma). After washing, the slides were incubated with secondary FITC anti-mouse IgG (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) at room temperature. Tissues were mounted, and images were acquired using a Nikon Eclipse 50i (Nikon Inc., Melville, NY, USA) fluorescence microscope (100× to 400×). Analyses of individual sections used in the final quantification were performed using Image-Pro Plus software (Media Cybernetics Inc., Silver Spring, MD, USA).

Hwang L., Ko I.G., Jin J.J., Kim S.H., Kim C.J., Jeon J.W, & Han J.H. (2018). Scolopendra subspinipes mutilans Extract Suppresses Inflammatory and Neuropathic Pain In Vitro and In Vivo. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 5057372.

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Histological Evaluation of Kidney, Lung, and Skin

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Kidneys, lungs and skin biopsies were fixed in 10% formaldehyde. Tissue sections were stained with H&E or Periodic acid-Schiff (PAS) and visualized by Nikon. Histological scores of skins and kidneys were evaluated as described(34 (link), 35 (link)). Kidneys and skins were scored from 6 mice in each group. At least 20 glomeruli and 10 tubular and vessel area in low power field are scored and calculated an average in each tissue section. Briefly, severity of glomeluronephritis, interstitial nephritis and perivascular infiltrates were graded in a semiquantitative (0–3+) manner. For skin, the degree of acanthosis, none (0) to marked thickened dermis (27); hyperkeratosis, none (0) to markedly increased keratin (2); inflammation, sparse (0) to heavy lymphocytic infiltrates (27); fibrosis, dermal collagen with normal (0) to markedly thickened (2); vessels, normal (0) to diffuse dilated (2); ulcer, absence (0) ore presence (1). For immunofluorescence, frozen kidney sections (5 μm thickness) were fixed in acetone and blocked with 1% normal goat serum in PBS. Diluted FITC-anti-mouse IgG (Jackson Immunoresearch) and FITC-anti-mouse C3(36 (link)) antibodies were incubated overnight at 4 °C, and staining visualized with a Nikon C1 confocal microscope.

Mizui M., Koga T., Lieberman L.A., Beltran J., Yoshida N., Johnson M.C., Tisch R, & Tsokos G.C. (2014). Interleukin-2 Protects Lupus-prone Mice from Multiple End-organ Damage by Limiting CD4−CD8− Interleukin-17-producing T-cells. Journal of immunology (Baltimore, Md. : 1950), 193(5), 2168-2177.

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Immunofluorescence Antibody Protocol

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The following antibodies were used at the indicated concentration and obtained from the indicated sources: rabbit anti-MVH (1 : 500, Abcam, ab13840); rabbit anti-RHAU (1 : 100, Protein Technologies, Inc., Tucson, AZ, USA, 13159-1-AP); rabbit anti-RHAU (1 : 200, Abcam, Shanghai, China, ab70269); rabbit anti-phosphor-histone-H2A.X (1 : 200, CST, Danvers, MA, USA, #9718); rabbit anti-Sycp3 (1 : 200, Abcam, ab15093); mouse anti-MLH1 (1 : 50, BD Pharmingen, Shanghai, China, 551092); mouse anti-PLZF (1 : 200, Santa Cruz Biotechnology, Inc., Dallas, TX, USA, sc-28319); rabbit anti-Lin28 (1 : 500, Santa Cruz Biotechnology, Inc., sc-67266); rabbit anti-c-kit (1 : 300, CST, #3074); mouse anti-β-actin (1 : 10000, Santa Cruz, Biotechnology, Inc., sc-81178); mouse anti-BrdU (1 : 100, EMD Millipore, Billerica, MA, USA, 05–633); goat anti-rabbit IgG (H+L)-HRP (1 : 5000, Bioworld, St. Louis Park, MN, USA, BS10350); goat anti-mouse IgG-HRP (1 : 5000, Abmart, Shanghai, China, #M21002); Cy3-anti-mouse IgG (1 : 200, Jackson Immunoresearch, West Grove, PA, USA, 115-165-146); FITC-anti-rabbit IgG (1 : 200, Jackson Immunoresearch, 111-095-144); Cy3-anti-rabbit IgG (1 : 200, Jackson Immunoresearch, 111-165-003); and FITC-anti-mouse IgG (1 : 200, Jackson Immunoresearch, 115-095-146).

Gao X., Ma W., Nie J., Zhang C., Zhang J., Yao G., Han J., Xu J., Hu B., Du Y., Shi Q., Yang Z., Huang X, & Zhang Y. (2015). A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation. Cell Death & Disease, 6(1), e1610-.

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RBC Antibody Binding Assay

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Serum RBC ab levels were detected as previously described (30 (link)). Freshly isolated RBCs were washed three times in PBS, and resuspended to 1% RBCs. 10 μl of 1% RBCs were incubated with anti-mouse IgM-FITC (1:150; on ice) or anti-mouse IgG-FITC (1:50; at 37 °C; Jackson ImmunoResearch). The %RBCs bound by ab was determined by flow cytometry.

Valentine K.M., Davini D., Lawrence T.J., Mullins G.N., Manansala M., Al-Kuhlani M., Pinney J.M., Davis J.K., Beaudin A.E., Sindi S.S., Gravano D.M, & Hoyer K.K. (2018). CD8 follicular T cells promote B cell antibody class-switch in autoimmune disease. Journal of immunology (Baltimore, Md. : 1950), 201(1), 31-40.

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Characterization of Prostate Cancer Cells

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Prostate tumor cell lines were cultured as described previously [14 (link)]. The F77 antibody was purified in our laboratory [13 (link)]. All cell lines were routinely checked for mycoplasma. PC3 cell lines were confirmed virus free by the IMPACT II test (IDEXX Bioresearch). Anti-CD44 (IM-7), biotinylated anti-CD44 (IM-7) and anti-mouse IgG were purchased from Biolegend. anti-mouse IgG FITC was purchased from Jackson ImmunoResearch. Anti-rat AF594 was purchased from Invitrogen.

Chen X., Nagai Y., Zhu Z., Ruan H., Peehl D.M., Greene M.I, & Zhang H. (2017). A spliced form of CD44 expresses the unique glycan that is recognized by the prostate cancer specific antibody F77. Oncotarget, 9(3), 3631-3640.

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Immunofluorescence analysis of iPSC-derived retinal organoids

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iPSC-derived retinal organoids were fixed in 4% paraformaldehyde at room temperature for 20 min. Post-fixation retinal organoids were incubated overnight with 30% sucrose in PBS, and then frozen and cryosectioned. The frozen sections were stained for a panel of retinal-specific antibodies. Antibodies against the following proteins were used at the indicated dilutions: RECOVERIN (Merck Millipore, AB5585, 1:800), NRL (Santa Cruz, sc-374277, 1:800), CRALBP (Abcam, ab15051, 1:500), HuC/D (ThermoFisher, A21271, 1:500), PKCα (BD Biosciences, 610107, 1:500), ARL13B (Proteintech, 17711-1-AP, 1:500), PRPF31 (Abnova, PAB7154, 1:500), SNRPB monoclonal antibody (Y12) (ThermoFisher, MA5-13449, 1:500). The following secondary antibodies were used: anti-mouse-IgG-FITC (Jackson Immuno Research, 715-095-151, 1:500), anti-mouse-IgG-Cy3 (Jackson Immuno Research/115-165-003, 1:500), anti-rabbit-IgG-Cy3 (Jackson Immuno Research/111-165-003, 1:500), anti-goat-IgG-FITC (Jackson Immuno Research/705-096-147, 1:500). Nuclei were labelled with blue-DAPI (ThermoFisher, 62248). All antibody details are shown in Supplementary Data 6.

Buskin A., Zhu L., Chichagova V., Basu B., Mozaffari-Jovin S., Dolan D., Droop A., Collin J., Bronstein R., Mehrotra S., Farkas M., Hilgen G., White K., Pan K.T., Treumann A., Hallam D., Bialas K., Chung G., Mellough C., Ding Y., Krasnogor N., Przyborski S., Zwolinski S., Al-Aama J., Alharthi S., Xu Y., Wheway G., Szymanska K., McKibbin M., Inglehearn C.F., Elliott D.J., Lindsay S., Ali R.R., Steel D.H., Armstrong L., Sernagor E., Urlaub H., Pierce E., Lührmann R., Grellscheid S.N., Johnson C.A, & Lako M. (2018). Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa. Nature Communications, 9, 4234.

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Quantifying Anti-Mouse RBC Antibodies

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Abs bound to RBCs were detected using flow cytometry as previously described^{18 (link)}. RBCs were freshly isolated from mice by terminal bleed or submandibular vein puncture, washed three times in room-temperature PBS, and resuspended to 1% RBCs. 10 μl of 1% RBCs were incubated with either anti-mouse IgM-FITC (1:100 dilution; on ice; Jackson ImmunoResearch) or anti-mouse IgG-FITC (1:50 dilution; at 37 °C; Jackson ImmunoResearch) for 20–30 min. The percentage of RBCs bound by Ab was determined by flow cytometry.

Mullins G.N., Valentine K.M., Al-Kuhlani M., Davini D., Jensen K.D, & Hoyer K.K. (2020). T cell signaling and Treg dysfunction correlate to disease kinetics in IL-2Rα-KO autoimmune mice. Scientific Reports, 10, 21994.

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Anti-MUC1 TRA mAb H23 as Positive Control

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The anti-MUC1 TRA mAb H23, raised against the human breast cancer cell line T47D, [35] (link) recognizing the non-glycosylated MUC1 epitope APDTRP, served as a positive control. Mouse anti-goat or rabbit anti-mouse IgG-FITC (Jackson ImmunoResearch, USA) served as a negative control for FACS analyses. Normal mouse or rabbit IgG antibodies (Chemicon, Millipore, USA) were used for complement-dependent cytotoxicity (CDC) analyses.

Kovjazin R., Horn G., Smorodinsky N.I., Shapira M.Y, & Carmon L. (2014). Cell Surface-Associated Anti-MUC1-Derived Signal Peptide Antibodies: Implications for Cancer Diagnostics and Therapy. PLoS ONE, 9(1), e85400.

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BrdU Incorporation Assay in Drosophila

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ptc-GAL4 or en-GAL4 females were crossed with UAS-RBF and UAS-RBF^D253A males, and with w¹¹¹⁸ males for the control. Larvae were fed for 2 h on medium supplemented with 1 mg/ml BrdU. They were then dissected in 1X PBS pH 7.6, and fixed in PBT/formaldehyde (PBS 1X/5% formaldehyde/0.3% Triton X-100) for 20 min at room temperature, washed three times for 31min in PBT, denatured in 2.2 N HCl/0.1% Triton X-100 by two 15 minute-long incubations, neutralized with 100 mM sodium tetraborate (Borax) by two 5 minute-long incubations. For immunohistochemistry, larvae were blocked by incubation in PBT/10% fetal calf serum (FCS) for 45 min, incubated with mouse anti-BrdU monoclonal antibody (1: 200, DSHB) overnight at 4°C and washed thrice in PBT/FCS. Discs were then incubated with anti-mouse IgG-FITC (1: 200, Jackson Immuno Research) and washed thrice in PBT. Discs were mounted in Citifluor™ and observed with a Leica SP2 upright confocal microscope.

Milet C., Rincheval-Arnold A., Moriéras A., Clavier A., Garrigue A., Mignotte B, & Guénal I. (2014). Mutating RBF Can Enhance Its Pro-Apoptotic Activity and Uncovers a New Role in Tissue Homeostasis. PLoS ONE, 9(8), e102902.

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Measurement of Anti-Donor Antibodies

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Serum was obtained from mice and diluted 1:100 in PBS. The serum was incubated with enriched allogeneic T cells (target cells; 2 × 10⁵ cells/well) in 96-well plates. After incubating for 1 h at 4µC, the cells were washed twice with PBS, re-suspended, and cultured with anti-mouse IgG FITC (Jackson Immuno Research West Grove, PA, USA) on ice for 30 min. The cells were washed twice and re-suspended in PBS, and then data were acquired on a flow cytometer. The degree of fluorescence was reported as median fluorescence intensity (MFI) as a measure of anti-donor antibody attachment to target cells.

Mori D.N., Shen H., Galan A, & Goldstein D.R. (2016). Aged B cells alter immune regulation of allografts. European journal of immunology, 46(11), 2650-2658.

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