Log In Sign up
The largest database of trusted experimental protocols

Staphylococcus aureus mrsa

Manufactured by American Type Culture Collection
Visit Supplier
Sourced in United States

Staphylococcus aureus MRSA is a bacterium commonly used in laboratory settings. It is a strain of Staphylococcus aureus that has developed resistance to many antibiotics, including methicillin. This strain is often used for research and testing purposes related to antimicrobial resistance and the development of new treatment methods.

Automatically generated - may contain errors

Lab products found in correlation

Candida albicans, by American Type Culture Collection (3 mentions) Escherichia coli, by American Type Culture Collection (3 mentions) Staphylococcus epidermidis, by American Type Culture Collection (2 mentions) Staphylococcus aureus mssa, by American Type Culture Collection (2 mentions) Pseudomonas aeruginosa, by American Type Culture Collection (2 mentions) Staphylococcus aureus, by American Type Culture Collection (2 mentions) Enterococcus hirae, by American Type Culture Collection (1 mentions) Staphylococcus aureus atcc 6538, by American Type Culture Collection (1 mentions) Enterococcus faecalis, by American Type Culture Collection (1 mentions) Streptococcus pyogenes, by American Type Culture Collection (1 mentions) Enterobacter cloacae, by American Type Culture Collection (1 mentions) Klebsiella pneumoniae, by American Type Culture Collection (1 mentions) Acinetobacter baumannii, by American Type Culture Collection (1 mentions) E coli o157 h7, by American Type Culture Collection (1 mentions) Enterococcus faecium, by American Type Culture Collection (1 mentions) Pseudomonas aeruginosa pao1, by American Type Culture Collection (1 mentions) Staphylococcus aureus rosenbach, by American Type Culture Collection (1 mentions)

6 protocols using staphylococcus aureus mrsa

1

Antimicrobial Susceptibility Testing of Bacterial and Fungal Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fourteen bacterial strains from the American Type Culture Collection (ATCC; Molsheim, France), the Pasteur Institute Collection (CIP; Paris, France), or from clinical samples (Escherichia coli UTI89 and extended‐spectrum beta‐lactamase positive [ESBL]) were tested. There were four Gram‐positive strains: Streptococcus pyogenes CIP 5641T, Streptococcus pneumoniae CIP 104471, Listeria monocytogenes CIP 82110T, and Staphylococcus aureus MRSA ATCC 33591; and ten Gram‐negative strains: Pseudomonas aeruginosa CIP 103467, Proteus mirabilis CIP 103181T, Escherichia coli ESBL, Escherichia coli UTI 89, Klebsiella pneumoniae CIP 8291T, Salmonella typhimurium CIP 6062T, Yersinia enterocolitica CIP 8027T, Bacteriodes fragilis ATCC 25285, Haemophilus influenza IP 102514, and Branhamella catarrhalis CIP 7321T. Pseudomonas aeruginosa CIP 103467, Staphylococcus aureus MRSA ATCC 3359, and Escherichia coli ESBL were selected for their marked natural or acquired resistance to antibiotics.
The following six fungal strains were tested as follows: Candida albicans DSM 1386, Candida glabatra DSM 11226, Candida tropicalis IP 2148.93, Candida albicans F26, Candida albicans F35, and Candida albicans F78. Two were from Deutsche Sammlung von Mikrooganismen und Zellkulturen (DSM), one from the Pasteur Institute (IP), and three were clinical isolates (F).
Brochot A., Guilbot A., Haddioui L, & Roques C. (2017). Antibacterial, antifungal, and antiviral effects of three essential oil blends. MicrobiologyOpen, 6(4), e00459.
+ Open protocol
+ Expand
2

Antimicrobial Activity Evaluation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
β-Hemolytic Streptococcus group A PCM 465, Streptococcus group G and Corynebacterium diphtheriae came from Department of Pharmaceutical Microbiology of Medical University of Gdańsk collection, Staphylococcus aureus ATCC6538, Staphylococcus aureus MRSA ATCC43300, Staphylococcus epidermidis ATCC14990, Enterococcus hirae ATCC10541, Escherichia coli ATCC8739 and Candida albicans ATCC10231 were obtained from the ATCC collection (ATCC, Manassas, VA, USA).
Stefanowicz-Hajduk J., Hering A., Gucwa M., Hałasa R., Soluch A., Kowalczyk M., Stochmal A, & Ochocka R. (2020). Biological activities of leaf extracts from selected Kalanchoe species and their relationship with bufadienolides content. Pharmaceutical Biology, 58(1), 732-740.
+ Open protocol
+ Expand
3

Antimicrobial Evaluation of Synthesized Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antimicrobial activity of the synthesized compounds was assessed against 11 microbial strains: 5 Gram-negative, Escherichia coli American Type Culture Collection (ATTC) 25922, Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 27853), Pseudomonas aeruginosa (wild-type strain isolated from clinical sample), Acinetobacter baumannii (wild-type strain isolated from clinical sample), and 6 Gram-positive, Enterococcus faecalis (ATCC 29212), Staphylococcus aureus MSSA (ATCC 25923), Staphylococcus aureus MRSA (ATCC 43300), Staphylococcus aureus MLSB of inducible phenotype with resistance to macrolide–lincosamide–streptogramin B antibiotics (wild-type strain isolated from clinical sample), Micrococcus luteus Polish Collection of Microorganisms (PCM) 1944, Streptococcus mutans (wild-type strain isolated from dental plaque). Bacterial strains were cultivated in Brain Heart Infusion (BHI, Oxoid) medium at 37 °C for 24 h. After incubation, microbial suspension was diluted with sterile phosphate-buffered saline (PBS) to 108 CFU/mL (turbidity = McFarland barium sulfate standard 0.5).
Goszczyńska A., Kwiecień H, & Fijałkowski K. (2015). Synthesis and antibacterial activity of Schiff bases and amines derived from alkyl 2-(2-formyl-4-nitrophenoxy)alkanoates. Medicinal Chemistry Research, 24(9), 3561-3577.
+ Open protocol
+ Expand
4

Antimicrobial Susceptibility Testing Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The strains Escherichia. coli (ATCC 25922), Klebisella pneumoniae (ATCC 13883), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus MSSA (ATCC 25923), Staphylococcus aureus MRSA (ATCC 43300), Streptococcus pyogenes (ATCC 19615) and Candida Albicans (ATCC 13883) were provided by Remel Microbiology (Thermo Fisher). The cells were grown in Müller-Hinton broth II (MHB; Difco, Detroit, MI, USA) containing 2g/l beef infusion solids, 17.5 g/l casein hydrolysate, 1.5 g/l starch. The final pH was adjusted to 7.4.
Ozsvari B., Fiorillo M., Bonuccelli G., Cappello A.R., Frattaruolo L., Sotgia F., Trowbridge R., Foster R, & Lisanti M.P. (2017). Mitoriboscins: Mitochondrial-based therapeutics targeting cancer stem cells (CSCs), bacteria and pathogenic yeast. Oncotarget, 8(40), 67457-67472.
+ Open protocol
+ Expand
5

Bacterial Strains Used in Study

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ten bacterial strains used in this study were obtained from Guangdong Microbial Culture Collection Center. E. coli MDR was a generous gift from Zhejiang Academy of Agricultural Sciences. The E. coli O157:H7 (ATCC 35 150), E. coli (ATCC 8739), E. coli MDR, Salmonella Typhimurium (ATCC 14 028), Klebsiella pneumoniae (ATCC 13 883), Acinetobacter baumannii (ATCC 17 978), Pseudomonas aeruginosa (ATCC 10 145), and Enterobacter cloacae (ATCC 13 047) were used as representative gram‐negative bacteria. The Enterococcus faecium (ATCC 19 434), Staphylococcus aureus (ATCC 25 923), and Staphylococcus aureus MRSA (ATCC 43 300) were used as representative gram‐positive bacteria. All strains were cultured in nutrient broth (NB) medium at 37 °C with shaking at 180 rpm before use.
He Q., Yang Z., Zou Z., Qian M., Wang X., Zhang X., Yin Z., Wang J., Ye X., Liu D, & Guo M. (2022). Combating Escherichia coli O157:H7 with Functionalized Chickpea‐Derived Antimicrobial Peptides. Advanced Science, 10(6), 2205301.
+ Open protocol
+ Expand
6

Microbial Strains Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
All microbes used in this work were purchased from American Type Culture Collection (ATCC, Manassas, VA): E. coli (ATCC-15597), Staphylococcus epidermidis (ATCC-14990), Staphylococcus aureus Rosenbach (ATCC-12600), Staphylococcus aureus MRSA (ATCC-BAA1754), Pseudomonas aeruginosa PAO1 (ATCC-10145), and Candida albicans (ATCC-18804). All strains were incubated at 37 °C at 150 rpm until a midexponential phase was acquired to harvest the cells by centrifugation at 3800 × g for 8 min. E. coli was grown on a Luria-Bertani (LB) broth; S. epidermidis, P. aeruginosa, and S. aureus Rosenbach were grown on nutrient broth; S. aureus (MRSA) was grown on a TSB; and C. albicans was grown on a yeast mold broth. These initial cultures were then adjusted to an optical density of 1 at 600 nm and had an initial total cell number ranging from 1 × 107 cells per mL to 1 × 108 cells per mL, as confirmed by plate counting.
McVerry B., Polasko A., Rao E., Haghniaz R., Chen D., He N., Ramos P., Hayashi J., Curson P., Wu C.Y., Bandaru P., Anderson M., Bui B., Sayegh A., Mahendra S., Di Carlo D., Kreydin E., Khademhosseini A., Sheikhi A, & Kaner R.B. (2022). A Readily Scalable, Clinically Demonstrated, Antibiofouling Zwitterionic Surface Treatment for Implantable Medical Devices. Advanced materials (Deerfield Beach, Fla.), 34(20), e2200254.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!